Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20749 and SM_b20750 are separated by 19 nucleotides SM_b20750 and SM_b20751 are separated by 30 nucleotides SM_b20751 and SM_b20752 are separated by 46 nucleotides
SM_b20749: SM_b20749 - D-mannonate oxidoreductase, at 1,538,076 to 1,539,539
_b20749
SM_b20750: SM_b20750 - dehydrogenase, at 1,539,559 to 1,540,308
_b20750
SM_b20751: SM_b20751 - 3-hydroxyisobutyrate dehydrogenase, at 1,540,339 to 1,541,235
_b20751
SM_b20752: SM_b20752 - 3-hydroxyisobutyryl-CoA hydrolase, at 1,541,282 to 1,542,352
_b20752
Position (kb) 1540
1541
1542 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1539.537 kb on + strand at 1540.015 kb on + strand, within SM_b20750 at 1540.018 kb on - strand, within SM_b20750 at 1540.235 kb on + strand at 1540.430 kb on + strand, within SM_b20751 at 1540.430 kb on + strand, within SM_b20751 at 1540.589 kb on + strand, within SM_b20751 at 1540.589 kb on + strand, within SM_b20751 at 1540.589 kb on + strand, within SM_b20751 at 1540.590 kb on - strand, within SM_b20751 at 1540.623 kb on + strand, within SM_b20751 at 1540.623 kb on + strand, within SM_b20751 at 1540.852 kb on - strand, within SM_b20751 at 1541.018 kb on + strand, within SM_b20751 at 1541.284 kb on - strand at 1541.284 kb on - strand at 1541.347 kb on + strand at 1541.856 kb on + strand, within SM_b20752 at 1541.856 kb on + strand, within SM_b20752 at 1541.856 kb on + strand, within SM_b20752 at 1541.857 kb on - strand, within SM_b20752 at 1541.860 kb on + strand, within SM_b20752 at 1542.032 kb on - strand, within SM_b20752 at 1542.033 kb on + strand, within SM_b20752 at 1542.033 kb on + strand, within SM_b20752 at 1542.034 kb on - strand, within SM_b20752 at 1542.046 kb on - strand, within SM_b20752 at 1542.127 kb on - strand, within SM_b20752 at 1542.127 kb on - strand, within SM_b20752 at 1542.128 kb on + strand, within SM_b20752 at 1542.129 kb on - strand, within SM_b20752
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,539,537 + +0.1 1,540,015 + SM_b20750 0.61 -1.7 1,540,018 - SM_b20750 0.61 -1.1 1,540,235 + +0.1 1,540,430 + SM_b20751 0.10 +0.2 1,540,430 + SM_b20751 0.10 +2.4 1,540,589 + SM_b20751 0.28 -2.9 1,540,589 + SM_b20751 0.28 -0.8 1,540,589 + SM_b20751 0.28 +1.2 1,540,590 - SM_b20751 0.28 +1.0 1,540,623 + SM_b20751 0.32 +0.5 1,540,623 + SM_b20751 0.32 +1.0 1,540,852 - SM_b20751 0.57 +1.1 1,541,018 + SM_b20751 0.76 +0.4 1,541,284 - -4.1 1,541,284 - -0.6 1,541,347 + +0.1 1,541,856 + SM_b20752 0.54 -4.0 1,541,856 + SM_b20752 0.54 +1.0 1,541,856 + SM_b20752 0.54 -2.2 1,541,857 - SM_b20752 0.54 -1.6 1,541,860 + SM_b20752 0.54 +0.3 1,542,032 - SM_b20752 0.70 -0.3 1,542,033 + SM_b20752 0.70 -3.1 1,542,033 + SM_b20752 0.70 +0.6 1,542,034 - SM_b20752 0.70 +0.6 1,542,046 - SM_b20752 0.71 +1.0 1,542,127 - SM_b20752 0.79 -2.9 1,542,127 - SM_b20752 0.79 +1.2 1,542,128 + SM_b20752 0.79 +0.1 1,542,129 - SM_b20752 0.79 +1.1
Or see this region's nucleotide sequence