Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20473 and SM_b20474 are separated by 556 nucleotides SM_b20474 and SM_b20475 overlap by 14 nucleotides SM_b20475 and SM_b20476 are separated by 339 nucleotides
SM_b20473: SM_b20473 - hypothetical protein, at 486,727 to 487,224
_b20473
SM_b20474: SM_b20474 - hypothetical protein, at 487,781 to 488,002
_b20474
SM_b20475: SM_b20475 - hypothetical protein, at 487,989 to 488,483
_b20475
SM_b20476: SM_b20476 - peptide ABC transporter substrate-binding protein, at 488,823 to 490,388
_b20476
Position (kb) 487
488
489 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 486.865 kb on + strand, within SM_b20473 at 486.925 kb on + strand, within SM_b20473 at 486.926 kb on - strand, within SM_b20473 at 487.032 kb on - strand, within SM_b20473 at 487.032 kb on - strand, within SM_b20473 at 487.032 kb on - strand, within SM_b20473 at 487.032 kb on - strand, within SM_b20473 at 487.104 kb on + strand, within SM_b20473 at 487.104 kb on + strand, within SM_b20473 at 487.105 kb on - strand, within SM_b20473 at 487.181 kb on + strand at 487.181 kb on + strand at 487.261 kb on + strand at 487.262 kb on - strand at 487.262 kb on - strand at 487.262 kb on - strand at 487.262 kb on - strand at 487.268 kb on - strand at 487.270 kb on + strand at 487.270 kb on + strand at 487.271 kb on - strand at 487.271 kb on - strand at 487.271 kb on - strand at 487.350 kb on + strand at 487.351 kb on - strand at 487.361 kb on + strand at 487.362 kb on - strand at 487.376 kb on - strand at 487.387 kb on + strand at 487.388 kb on - strand at 487.388 kb on - strand at 487.388 kb on - strand at 487.388 kb on - strand at 487.388 kb on - strand at 487.389 kb on + strand at 487.389 kb on + strand at 487.390 kb on - strand at 487.390 kb on - strand at 487.391 kb on + strand at 487.392 kb on - strand at 487.392 kb on - strand at 487.635 kb on + strand at 487.653 kb on + strand at 487.654 kb on - strand at 487.654 kb on - strand at 487.774 kb on + strand at 487.775 kb on - strand at 487.870 kb on + strand, within SM_b20474 at 487.944 kb on + strand, within SM_b20474 at 488.040 kb on + strand, within SM_b20475 at 488.040 kb on + strand, within SM_b20475 at 488.041 kb on - strand, within SM_b20475 at 488.119 kb on - strand, within SM_b20475 at 488.156 kb on + strand, within SM_b20475 at 488.216 kb on - strand, within SM_b20475 at 488.423 kb on - strand, within SM_b20475 at 488.423 kb on - strand, within SM_b20475 at 488.455 kb on + strand at 488.460 kb on + strand at 488.461 kb on - strand at 488.477 kb on + strand at 488.477 kb on + strand at 488.478 kb on - strand at 488.535 kb on + strand at 488.535 kb on + strand at 488.535 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 486,865 + SM_b20473 0.28 +0.2 486,925 + SM_b20473 0.40 +0.8 486,926 - SM_b20473 0.40 -3.8 487,032 - SM_b20473 0.61 +1.0 487,032 - SM_b20473 0.61 -2.2 487,032 - SM_b20473 0.61 -0.2 487,032 - SM_b20473 0.61 -2.6 487,104 + SM_b20473 0.76 -1.8 487,104 + SM_b20473 0.76 -1.2 487,105 - SM_b20473 0.76 -0.1 487,181 + +0.1 487,181 + +1.1 487,261 + +0.0 487,262 - -0.1 487,262 - -0.4 487,262 - +0.1 487,262 - -1.1 487,268 - -0.3 487,270 + +0.2 487,270 + -1.7 487,271 - -1.3 487,271 - +0.8 487,271 - -3.1 487,350 + -0.1 487,351 - -2.6 487,361 + +0.2 487,362 - +0.6 487,376 - +1.3 487,387 + -0.6 487,388 - +0.5 487,388 - -0.6 487,388 - +0.4 487,388 - -0.9 487,388 - -0.0 487,389 + -0.2 487,389 + -0.8 487,390 - -1.7 487,390 - -3.0 487,391 + -0.2 487,392 - +1.1 487,392 - +0.5 487,635 + +0.1 487,653 + -1.2 487,654 - -0.6 487,654 - +1.0 487,774 + -1.3 487,775 - +0.3 487,870 + SM_b20474 0.40 +1.1 487,944 + SM_b20474 0.73 +1.0 488,040 + SM_b20475 0.10 -0.7 488,040 + SM_b20475 0.10 +0.1 488,041 - SM_b20475 0.11 +1.6 488,119 - SM_b20475 0.26 +1.1 488,156 + SM_b20475 0.34 -1.5 488,216 - SM_b20475 0.46 +0.0 488,423 - SM_b20475 0.88 -0.9 488,423 - SM_b20475 0.88 -1.0 488,455 + +0.3 488,460 + +1.0 488,461 - -1.2 488,477 + +1.3 488,477 + -0.3 488,478 - +1.6 488,535 + -2.5 488,535 + -1.7 488,535 + -0.5
Or see this region's nucleotide sequence