Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20030 and SM_b20031 are separated by 25 nucleotides SM_b20031 and SM_b20032 are separated by 96 nucleotides SM_b20032 and SM_b20033 are separated by 389 nucleotides
SM_b20030: SM_b20030 - RNA polymerase sigma factor SigJ, at 41,423 to 42,280
_b20030
SM_b20031: SM_b20031 - hypothetical protein, at 42,306 to 42,554
_b20031
SM_b20032: SM_b20032 - hypothetical protein, at 42,651 to 43,385
_b20032
SM_b20033: SM_b20033 - transcriptional regulator, at 43,775 to 44,437
_b20033
Position (kb) 42
43
44 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 41.666 kb on + strand, within SM_b20030 at 41.666 kb on + strand, within SM_b20030 at 41.666 kb on + strand, within SM_b20030 at 41.666 kb on + strand, within SM_b20030 at 41.667 kb on - strand, within SM_b20030 at 41.667 kb on - strand, within SM_b20030 at 42.239 kb on + strand at 42.239 kb on + strand at 42.307 kb on + strand at 42.372 kb on - strand, within SM_b20031 at 42.372 kb on - strand, within SM_b20031 at 42.372 kb on - strand, within SM_b20031 at 42.372 kb on - strand, within SM_b20031 at 42.500 kb on - strand, within SM_b20031 at 42.500 kb on - strand, within SM_b20031 at 42.583 kb on + strand at 42.583 kb on + strand at 42.584 kb on - strand at 42.776 kb on - strand, within SM_b20032 at 42.825 kb on + strand, within SM_b20032 at 42.825 kb on + strand, within SM_b20032 at 42.826 kb on - strand, within SM_b20032 at 43.053 kb on - strand, within SM_b20032 at 43.275 kb on + strand, within SM_b20032 at 43.275 kb on + strand, within SM_b20032 at 43.275 kb on + strand, within SM_b20032 at 43.276 kb on - strand, within SM_b20032 at 43.276 kb on - strand, within SM_b20032 at 43.276 kb on - strand, within SM_b20032 at 43.358 kb on - strand at 43.358 kb on - strand at 43.358 kb on - strand at 43.537 kb on + strand at 43.537 kb on + strand at 43.538 kb on - strand at 43.538 kb on - strand at 43.538 kb on - strand at 43.675 kb on - strand at 43.777 kb on - strand at 43.777 kb on - strand at 43.813 kb on - strand at 43.813 kb on - strand at 44.068 kb on + strand, within SM_b20033 at 44.068 kb on + strand, within SM_b20033 at 44.250 kb on + strand, within SM_b20033 at 44.277 kb on + strand, within SM_b20033 at 44.277 kb on + strand, within SM_b20033 at 44.280 kb on + strand, within SM_b20033
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 41,666 + SM_b20030 0.28 -0.6 41,666 + SM_b20030 0.28 -0.5 41,666 + SM_b20030 0.28 +0.5 41,666 + SM_b20030 0.28 -1.7 41,667 - SM_b20030 0.28 +1.4 41,667 - SM_b20030 0.28 -2.8 42,239 + +0.1 42,239 + +1.3 42,307 + -2.0 42,372 - SM_b20031 0.27 -0.1 42,372 - SM_b20031 0.27 +0.2 42,372 - SM_b20031 0.27 +2.1 42,372 - SM_b20031 0.27 +0.3 42,500 - SM_b20031 0.78 -1.2 42,500 - SM_b20031 0.78 -0.9 42,583 + +0.4 42,583 + -1.3 42,584 - -2.8 42,776 - SM_b20032 0.17 +0.1 42,825 + SM_b20032 0.24 +0.1 42,825 + SM_b20032 0.24 +0.7 42,826 - SM_b20032 0.24 +1.0 43,053 - SM_b20032 0.55 +0.5 43,275 + SM_b20032 0.85 +3.3 43,275 + SM_b20032 0.85 -1.1 43,275 + SM_b20032 0.85 -0.5 43,276 - SM_b20032 0.85 +0.5 43,276 - SM_b20032 0.85 +0.1 43,276 - SM_b20032 0.85 +1.3 43,358 - -0.2 43,358 - -0.1 43,358 - +0.7 43,537 + +0.5 43,537 + +2.4 43,538 - -0.2 43,538 - +1.2 43,538 - +0.9 43,675 - -2.1 43,777 - -2.8 43,777 - -1.2 43,813 - -0.9 43,813 - +2.3 44,068 + SM_b20033 0.44 -0.2 44,068 + SM_b20033 0.44 +1.6 44,250 + SM_b20033 0.72 -0.8 44,277 + SM_b20033 0.76 -0.1 44,277 + SM_b20033 0.76 -0.2 44,280 + SM_b20033 0.76 +0.1
Or see this region's nucleotide sequence