Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04340 and HSERO_RS04345 are separated by 196 nucleotides HSERO_RS04345 and HSERO_RS04350 are separated by 191 nucleotides HSERO_RS04350 and HSERO_RS04355 are separated by 298 nucleotides
HSERO_RS04340: HSERO_RS04340 - gamma-glutamyl ligase, at 958,999 to 960,228
_RS04340
HSERO_RS04345: HSERO_RS04345 - membrane protein, at 960,425 to 961,633
_RS04345
HSERO_RS04350: HSERO_RS04350 - 30S ribosomal protein S21, at 961,825 to 962,037
_RS04350
HSERO_RS04355: HSERO_RS04355 - aspartyl-tRNA amidotransferase subunit B, at 962,336 to 962,782
_RS04355
Position (kb) 960
961
962 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 959.462 kb on + strand, within HSERO_RS04340 at 959.548 kb on + strand, within HSERO_RS04340 at 959.604 kb on - strand, within HSERO_RS04340 at 959.704 kb on + strand, within HSERO_RS04340 at 959.921 kb on - strand, within HSERO_RS04340 at 959.942 kb on + strand, within HSERO_RS04340 at 960.200 kb on + strand at 960.287 kb on + strand at 960.295 kb on - strand at 960.390 kb on + strand at 960.390 kb on + strand at 960.390 kb on + strand at 960.535 kb on - strand at 960.553 kb on + strand, within HSERO_RS04345 at 960.694 kb on + strand, within HSERO_RS04345 at 960.786 kb on - strand, within HSERO_RS04345 at 960.830 kb on + strand, within HSERO_RS04345 at 960.830 kb on + strand, within HSERO_RS04345 at 961.060 kb on + strand, within HSERO_RS04345 at 961.093 kb on - strand, within HSERO_RS04345 at 961.209 kb on + strand, within HSERO_RS04345 at 961.437 kb on + strand, within HSERO_RS04345 at 961.594 kb on + strand at 961.628 kb on - strand at 962.316 kb on + strand at 962.401 kb on + strand, within HSERO_RS04355 at 962.533 kb on + strand, within HSERO_RS04355
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 959,462 + HSERO_RS04340 0.38 -2.1 959,548 + HSERO_RS04340 0.45 +1.3 959,604 - HSERO_RS04340 0.49 -3.5 959,704 + HSERO_RS04340 0.57 -2.6 959,921 - HSERO_RS04340 0.75 -1.2 959,942 + HSERO_RS04340 0.77 -2.2 960,200 + -1.0 960,287 + +1.5 960,295 - +0.6 960,390 + +0.3 960,390 + -0.2 960,390 + -0.2 960,535 - +0.1 960,553 + HSERO_RS04345 0.11 -0.3 960,694 + HSERO_RS04345 0.22 -0.2 960,786 - HSERO_RS04345 0.30 +0.3 960,830 + HSERO_RS04345 0.33 -0.7 960,830 + HSERO_RS04345 0.33 +0.2 961,060 + HSERO_RS04345 0.53 -0.9 961,093 - HSERO_RS04345 0.55 -0.3 961,209 + HSERO_RS04345 0.65 -0.9 961,437 + HSERO_RS04345 0.84 -0.3 961,594 + -0.2 961,628 - -0.3 962,316 + +0.3 962,401 + HSERO_RS04355 0.15 +0.4 962,533 + HSERO_RS04355 0.44 +0.0
Or see this region's nucleotide sequence