Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00500 and BPHYT_RS00505 are separated by 184 nucleotides BPHYT_RS00505 and BPHYT_RS00510 are separated by 51 nucleotides BPHYT_RS00510 and BPHYT_RS00515 are separated by 54 nucleotides BPHYT_RS00515 and BPHYT_RS00520 are separated by 193 nucleotides
BPHYT_RS00500: BPHYT_RS00500 - hypothetical protein, at 121,647 to 122,987
_RS00500
BPHYT_RS00505: BPHYT_RS00505 - glyoxalase, at 123,172 to 123,570
_RS00505
BPHYT_RS00510: BPHYT_RS00510 - S-(hydroxymethyl)glutathione dehydrogenase, at 123,622 to 124,731
_RS00510
BPHYT_RS00515: BPHYT_RS00515 - regulator, at 124,786 to 125,061
_RS00515
BPHYT_RS00520: BPHYT_RS00520 - glutamate--cysteine ligase, at 125,255 to 126,853
_RS00520
Position (kb) 123
124
125 Strain fitness (log2 ratio) -2
-1
0
1
2 at 122.668 kb on + strand, within BPHYT_RS00500 at 122.668 kb on + strand, within BPHYT_RS00500 at 122.669 kb on - strand, within BPHYT_RS00500 at 122.669 kb on - strand, within BPHYT_RS00500 at 122.839 kb on - strand, within BPHYT_RS00500 at 122.912 kb on - strand at 122.963 kb on + strand at 122.964 kb on - strand at 122.964 kb on - strand at 122.964 kb on - strand at 123.020 kb on + strand at 123.057 kb on + strand at 123.058 kb on - strand at 123.125 kb on + strand at 123.125 kb on + strand at 123.185 kb on + strand at 123.221 kb on + strand, within BPHYT_RS00505 at 123.231 kb on - strand, within BPHYT_RS00505 at 123.419 kb on + strand, within BPHYT_RS00505 at 123.420 kb on - strand, within BPHYT_RS00505 at 123.605 kb on - strand at 123.605 kb on - strand at 124.010 kb on + strand, within BPHYT_RS00510 at 124.047 kb on + strand, within BPHYT_RS00510 at 124.047 kb on + strand, within BPHYT_RS00510 at 124.295 kb on - strand, within BPHYT_RS00510 at 124.328 kb on + strand, within BPHYT_RS00510 at 124.329 kb on - strand, within BPHYT_RS00510 at 124.352 kb on + strand, within BPHYT_RS00510 at 124.369 kb on - strand, within BPHYT_RS00510 at 124.608 kb on + strand, within BPHYT_RS00510 at 124.747 kb on - strand at 125.054 kb on + strand at 125.082 kb on - strand at 125.095 kb on + strand at 125.218 kb on - strand at 125.256 kb on + strand at 125.256 kb on + strand at 125.398 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X remove 122,668 + BPHYT_RS00500 0.76 -0.3 122,668 + BPHYT_RS00500 0.76 -0.5 122,669 - BPHYT_RS00500 0.76 -1.2 122,669 - BPHYT_RS00500 0.76 +0.7 122,839 - BPHYT_RS00500 0.89 +0.2 122,912 - -0.4 122,963 + -1.8 122,964 - -0.8 122,964 - -0.6 122,964 - -0.4 123,020 + +2.7 123,057 + -0.0 123,058 - +0.1 123,125 + -2.3 123,125 + +0.0 123,185 + -0.2 123,221 + BPHYT_RS00505 0.12 -0.4 123,231 - BPHYT_RS00505 0.15 -0.3 123,419 + BPHYT_RS00505 0.62 +0.0 123,420 - BPHYT_RS00505 0.62 -1.1 123,605 - +0.6 123,605 - -0.8 124,010 + BPHYT_RS00510 0.35 +0.4 124,047 + BPHYT_RS00510 0.38 -0.4 124,047 + BPHYT_RS00510 0.38 -0.6 124,295 - BPHYT_RS00510 0.61 +1.8 124,328 + BPHYT_RS00510 0.64 -0.1 124,329 - BPHYT_RS00510 0.64 -0.1 124,352 + BPHYT_RS00510 0.66 +0.3 124,369 - BPHYT_RS00510 0.67 -0.1 124,608 + BPHYT_RS00510 0.89 -0.3 124,747 - -0.0 125,054 + +0.5 125,082 - -0.4 125,095 + -0.6 125,218 - +0.8 125,256 + -0.3 125,256 + +0.3 125,398 + +2.0
Or see this region's nucleotide sequence