Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc01008 and SMc01009 overlap by 4 nucleotides SMc01009 and SMc01010 are separated by 108 nucleotides SMc01010 and SMc01011 are separated by 204 nucleotides
SMc01008: SMc01008 - hypothetical protein, at 1,533,611 to 1,534,048
SMc01008
SMc01009: SMc01009 - hypothetical protein, at 1,534,045 to 1,534,584
SMc01009
SMc01010: SMc01010 - threonyl-tRNA synthetase, at 1,534,693 to 1,536,678
SMc01010
SMc01011: SMc01011 - hypothetical protein, at 1,536,883 to 1,537,878
SMc01011
Position (kb)
1534
1535
1536
1537 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1533.719 kb on + strand, within SMc01008 at 1533.719 kb on + strand, within SMc01008 at 1533.721 kb on + strand, within SMc01008 at 1533.722 kb on - strand, within SMc01008 at 1533.722 kb on - strand, within SMc01008 at 1533.722 kb on - strand, within SMc01008 at 1533.722 kb on - strand, within SMc01008 at 1533.722 kb on - strand, within SMc01008 at 1533.722 kb on - strand, within SMc01008 at 1533.743 kb on + strand, within SMc01008 at 1533.743 kb on + strand, within SMc01008 at 1533.743 kb on + strand, within SMc01008 at 1533.946 kb on - strand, within SMc01008 at 1533.946 kb on - strand, within SMc01008 at 1534.054 kb on + strand at 1534.103 kb on + strand, within SMc01009 at 1534.104 kb on - strand, within SMc01009 at 1534.104 kb on - strand, within SMc01009 at 1534.104 kb on - strand, within SMc01009 at 1534.440 kb on - strand, within SMc01009 at 1534.553 kb on + strand at 1534.554 kb on - strand at 1534.554 kb on - strand at 1534.554 kb on - strand at 1534.598 kb on + strand at 1534.655 kb on + strand at 1534.655 kb on + strand at 1534.655 kb on + strand at 1534.684 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,533,719 + SMc01008 0.25 -0.1 1,533,719 + SMc01008 0.25 +0.9 1,533,721 + SMc01008 0.25 -0.4 1,533,722 - SMc01008 0.25 +0.4 1,533,722 - SMc01008 0.25 -0.3 1,533,722 - SMc01008 0.25 +1.0 1,533,722 - SMc01008 0.25 -0.5 1,533,722 - SMc01008 0.25 -0.1 1,533,722 - SMc01008 0.25 -0.1 1,533,743 + SMc01008 0.30 +0.5 1,533,743 + SMc01008 0.30 -0.1 1,533,743 + SMc01008 0.30 +0.3 1,533,946 - SMc01008 0.76 -1.7 1,533,946 - SMc01008 0.76 -1.0 1,534,054 + -0.6 1,534,103 + SMc01009 0.11 -0.1 1,534,104 - SMc01009 0.11 +2.7 1,534,104 - SMc01009 0.11 -0.9 1,534,104 - SMc01009 0.11 -0.3 1,534,440 - SMc01009 0.73 -0.7 1,534,553 + -1.0 1,534,554 - -0.1 1,534,554 - +0.2 1,534,554 - -0.9 1,534,598 + +0.1 1,534,655 + -0.5 1,534,655 + -1.2 1,534,655 + -2.4 1,534,684 + -1.5
Or see this region's nucleotide sequence