Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1933 and SMa1935 are separated by 154 nucleotides SMa1935 and SMa1937 overlap by 4 nucleotides SMa1937 and SMa1939 are separated by 175 nucleotides
SMa1933: SMa1933 - LysR family transcriptional regulator, at 1,098,786 to 1,099,427
SMa1933
SMa1935: SMa1935 - TrpR binding protein WrbA, at 1,099,582 to 1,100,220
SMa1935
SMa1937: SMa1937 - hypothetical protein, at 1,100,217 to 1,101,455
SMa1937
SMa1939: SMa1939 - hypothetical protein, at 1,101,631 to 1,103,166
SMa1939
Position (kb)
1100
1101
1102 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1099.225 kb on - strand, within SMa1933 at 1099.432 kb on + strand at 1099.432 kb on + strand at 1099.432 kb on + strand at 1099.440 kb on - strand at 1099.468 kb on + strand at 1099.494 kb on + strand at 1099.494 kb on + strand at 1099.495 kb on - strand at 1099.607 kb on - strand at 1099.616 kb on - strand at 1099.787 kb on - strand, within SMa1935 at 1100.006 kb on + strand, within SMa1935 at 1100.006 kb on + strand, within SMa1935 at 1100.007 kb on - strand, within SMa1935 at 1100.007 kb on - strand, within SMa1935 at 1100.035 kb on - strand, within SMa1935 at 1100.063 kb on - strand, within SMa1935 at 1100.309 kb on + strand at 1100.668 kb on + strand, within SMa1937 at 1100.668 kb on + strand, within SMa1937 at 1100.755 kb on - strand, within SMa1937 at 1100.875 kb on + strand, within SMa1937 at 1100.876 kb on - strand, within SMa1937 at 1101.029 kb on - strand, within SMa1937 at 1101.114 kb on - strand, within SMa1937 at 1101.135 kb on + strand, within SMa1937 at 1101.431 kb on + strand at 1101.432 kb on - strand at 1101.524 kb on - strand at 1101.627 kb on - strand at 1101.627 kb on - strand at 1101.632 kb on + strand at 1101.632 kb on + strand at 1101.632 kb on + strand at 1101.632 kb on + strand at 1101.652 kb on - strand at 1101.652 kb on - strand at 1101.667 kb on - strand at 1101.747 kb on - strand at 1101.747 kb on - strand at 1101.747 kb on - strand at 1101.865 kb on - strand, within SMa1939 at 1101.919 kb on + strand, within SMa1939 at 1101.926 kb on + strand, within SMa1939 at 1101.927 kb on - strand, within SMa1939 at 1101.934 kb on + strand, within SMa1939 at 1102.187 kb on + strand, within SMa1939 at 1102.188 kb on - strand, within SMa1939 at 1102.203 kb on + strand, within SMa1939 at 1102.347 kb on - strand, within SMa1939
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,099,225 - SMa1933 0.68 -0.1 1,099,432 + +0.7 1,099,432 + +4.0 1,099,432 + -0.2 1,099,440 - +1.7 1,099,468 + -0.3 1,099,494 + +0.1 1,099,494 + +0.8 1,099,495 - +0.7 1,099,607 - +0.5 1,099,616 - -0.2 1,099,787 - SMa1935 0.32 -0.9 1,100,006 + SMa1935 0.66 -0.7 1,100,006 + SMa1935 0.66 -0.7 1,100,007 - SMa1935 0.67 -1.2 1,100,007 - SMa1935 0.67 +0.6 1,100,035 - SMa1935 0.71 +1.2 1,100,063 - SMa1935 0.75 -1.1 1,100,309 + +0.5 1,100,668 + SMa1937 0.36 -1.5 1,100,668 + SMa1937 0.36 -0.2 1,100,755 - SMa1937 0.43 -1.8 1,100,875 + SMa1937 0.53 -0.9 1,100,876 - SMa1937 0.53 -0.4 1,101,029 - SMa1937 0.66 -0.2 1,101,114 - SMa1937 0.72 +0.1 1,101,135 + SMa1937 0.74 -0.1 1,101,431 + -0.8 1,101,432 - +1.6 1,101,524 - -0.6 1,101,627 - -0.1 1,101,627 - -1.1 1,101,632 + +0.4 1,101,632 + -1.0 1,101,632 + -1.5 1,101,632 + +0.4 1,101,652 - +0.4 1,101,652 - +0.1 1,101,667 - -0.4 1,101,747 - -2.1 1,101,747 - +1.6 1,101,747 - +1.7 1,101,865 - SMa1939 0.15 -4.2 1,101,919 + SMa1939 0.19 -0.5 1,101,926 + SMa1939 0.19 -0.5 1,101,927 - SMa1939 0.19 -0.1 1,101,934 + SMa1939 0.20 -1.8 1,102,187 + SMa1939 0.36 -0.4 1,102,188 - SMa1939 0.36 +1.8 1,102,203 + SMa1939 0.37 +0.3 1,102,347 - SMa1939 0.47 +2.6
Or see this region's nucleotide sequence