Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21495 and SM_b21496 overlap by 4 nucleotides SM_b21496 and SM_b21497 are separated by 294 nucleotides SM_b21497 and SM_b21498 are separated by 3 nucleotides
SM_b21495: SM_b21495 - protease, at 1,417,062 to 1,417,565
_b21495
SM_b21496: SM_b21496 - protease, at 1,417,562 to 1,418,203
_b21496
SM_b21497: SM_b21497 - acriflavin resistance protein, at 1,418,498 to 1,419,619
_b21497
SM_b21498: SM_b21498 - acriflavin resistance protein, at 1,419,623 to 1,422,856
_b21498
Position (kb)
1418
1419
1420 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1417.500 kb on + strand, within SM_b21495 at 1417.500 kb on + strand, within SM_b21495 at 1417.500 kb on + strand, within SM_b21495 at 1417.501 kb on - strand, within SM_b21495 at 1417.528 kb on - strand at 1417.533 kb on + strand at 1417.595 kb on + strand at 1417.595 kb on + strand at 1417.595 kb on + strand at 1417.595 kb on + strand at 1417.595 kb on + strand at 1417.596 kb on - strand at 1417.596 kb on - strand at 1417.596 kb on - strand at 1417.596 kb on - strand at 1417.596 kb on - strand at 1417.596 kb on - strand at 1417.596 kb on - strand at 1417.618 kb on + strand at 1417.635 kb on - strand, within SM_b21496 at 1417.745 kb on + strand, within SM_b21496 at 1417.761 kb on - strand, within SM_b21496 at 1417.762 kb on + strand, within SM_b21496 at 1417.762 kb on + strand, within SM_b21496 at 1417.763 kb on - strand, within SM_b21496 at 1417.988 kb on + strand, within SM_b21496 at 1417.988 kb on + strand, within SM_b21496 at 1417.988 kb on + strand, within SM_b21496 at 1417.988 kb on + strand, within SM_b21496 at 1417.988 kb on + strand, within SM_b21496 at 1418.001 kb on - strand, within SM_b21496 at 1418.085 kb on - strand, within SM_b21496 at 1418.146 kb on - strand at 1418.151 kb on + strand at 1418.151 kb on + strand at 1418.151 kb on + strand at 1418.191 kb on + strand at 1418.192 kb on - strand at 1418.239 kb on - strand at 1418.255 kb on - strand at 1418.783 kb on + strand, within SM_b21497 at 1418.890 kb on + strand, within SM_b21497 at 1418.891 kb on - strand, within SM_b21497 at 1419.000 kb on - strand, within SM_b21497 at 1419.116 kb on + strand, within SM_b21497 at 1419.116 kb on + strand, within SM_b21497 at 1419.116 kb on + strand, within SM_b21497 at 1419.117 kb on - strand, within SM_b21497 at 1419.146 kb on + strand, within SM_b21497 at 1419.249 kb on + strand, within SM_b21497 at 1419.250 kb on - strand, within SM_b21497 at 1419.546 kb on + strand at 1419.547 kb on - strand at 1419.854 kb on + strand at 1419.887 kb on + strand at 1419.888 kb on - strand at 1419.945 kb on + strand at 1419.945 kb on + strand at 1419.946 kb on - strand at 1420.094 kb on + strand, within SM_b21498 at 1420.094 kb on + strand, within SM_b21498 at 1420.094 kb on + strand, within SM_b21498 at 1420.173 kb on - strand, within SM_b21498 at 1420.502 kb on + strand, within SM_b21498 at 1420.503 kb on - strand, within SM_b21498 at 1420.595 kb on + strand, within SM_b21498 at 1420.596 kb on - strand, within SM_b21498 at 1420.596 kb on - strand, within SM_b21498 at 1420.596 kb on - strand, within SM_b21498 at 1420.596 kb on - strand, within SM_b21498 at 1420.596 kb on - strand, within SM_b21498 at 1420.596 kb on - strand, within SM_b21498 at 1420.596 kb on - strand, within SM_b21498 at 1420.607 kb on + strand, within SM_b21498 at 1420.608 kb on - strand, within SM_b21498
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,417,500 + SM_b21495 0.87 -1.7 1,417,500 + SM_b21495 0.87 -0.6 1,417,500 + SM_b21495 0.87 -1.0 1,417,501 - SM_b21495 0.87 +0.1 1,417,528 - -1.3 1,417,533 + +0.5 1,417,595 + +0.7 1,417,595 + +2.2 1,417,595 + +0.2 1,417,595 + +1.1 1,417,595 + +0.6 1,417,596 - +0.7 1,417,596 - -1.9 1,417,596 - -0.4 1,417,596 - -3.4 1,417,596 - +0.5 1,417,596 - +0.5 1,417,596 - +0.4 1,417,618 + +0.0 1,417,635 - SM_b21496 0.11 +0.2 1,417,745 + SM_b21496 0.29 -0.6 1,417,761 - SM_b21496 0.31 -1.0 1,417,762 + SM_b21496 0.31 +0.1 1,417,762 + SM_b21496 0.31 -0.3 1,417,763 - SM_b21496 0.31 +0.7 1,417,988 + SM_b21496 0.66 +0.8 1,417,988 + SM_b21496 0.66 -0.2 1,417,988 + SM_b21496 0.66 -1.9 1,417,988 + SM_b21496 0.66 -1.1 1,417,988 + SM_b21496 0.66 +1.8 1,418,001 - SM_b21496 0.68 -2.2 1,418,085 - SM_b21496 0.81 -0.7 1,418,146 - +1.3 1,418,151 + +0.5 1,418,151 + +0.4 1,418,151 + -0.0 1,418,191 + -1.2 1,418,192 - -1.0 1,418,239 - -1.6 1,418,255 - +1.0 1,418,783 + SM_b21497 0.25 +3.3 1,418,890 + SM_b21497 0.35 +1.1 1,418,891 - SM_b21497 0.35 -1.0 1,419,000 - SM_b21497 0.45 +1.5 1,419,116 + SM_b21497 0.55 +2.0 1,419,116 + SM_b21497 0.55 -0.5 1,419,116 + SM_b21497 0.55 -1.0 1,419,117 - SM_b21497 0.55 -0.0 1,419,146 + SM_b21497 0.58 -1.7 1,419,249 + SM_b21497 0.67 -0.9 1,419,250 - SM_b21497 0.67 -0.2 1,419,546 + -1.2 1,419,547 - -0.0 1,419,854 + -0.2 1,419,887 + +0.2 1,419,888 - -0.2 1,419,945 + -0.5 1,419,945 + +0.3 1,419,946 - +0.8 1,420,094 + SM_b21498 0.15 +1.3 1,420,094 + SM_b21498 0.15 +0.5 1,420,094 + SM_b21498 0.15 -0.8 1,420,173 - SM_b21498 0.17 -1.2 1,420,502 + SM_b21498 0.27 +0.9 1,420,503 - SM_b21498 0.27 -0.6 1,420,595 + SM_b21498 0.30 +4.1 1,420,596 - SM_b21498 0.30 -0.3 1,420,596 - SM_b21498 0.30 +0.6 1,420,596 - SM_b21498 0.30 -0.0 1,420,596 - SM_b21498 0.30 -1.9 1,420,596 - SM_b21498 0.30 -0.2 1,420,596 - SM_b21498 0.30 -1.5 1,420,596 - SM_b21498 0.30 -2.8 1,420,607 + SM_b21498 0.30 -0.6 1,420,608 - SM_b21498 0.30 +0.2
Or see this region's nucleotide sequence