Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20625 and SM_b20626 are separated by 75 nucleotides SM_b20626 and SM_b20627 are separated by 34 nucleotides SM_b20627 and SM_b20628 overlap by 4 nucleotides SM_b20628 and SM_b20629 are separated by 85 nucleotides
SM_b20625: SM_b20625 - hypothetical protein, at 1,645,604 to 1,646,809
_b20625
SM_b20626: SM_b20626 - AraC family transcriptional regulator, at 1,646,885 to 1,647,871
_b20626
SM_b20627: SM_b20627 - hypothetical protein, at 1,647,906 to 1,648,214
_b20627
SM_b20628: SM_b20628 - hypothetical protein, at 1,648,211 to 1,648,480
_b20628
SM_b20629: SM_b20629 - hypothetical protein, at 1,648,566 to 1,648,844
_b20629
Position (kb)
1646
1647
1648 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1646.082 kb on + strand, within SM_b20625 at 1646.082 kb on + strand, within SM_b20625 at 1646.083 kb on - strand, within SM_b20625 at 1646.083 kb on - strand, within SM_b20625 at 1646.083 kb on - strand, within SM_b20625 at 1646.083 kb on - strand, within SM_b20625 at 1646.083 kb on - strand, within SM_b20625 at 1646.297 kb on - strand, within SM_b20625 at 1646.298 kb on + strand, within SM_b20625 at 1646.307 kb on - strand, within SM_b20625 at 1646.801 kb on - strand at 1646.810 kb on - strand at 1646.810 kb on - strand at 1646.839 kb on + strand at 1646.843 kb on + strand at 1646.843 kb on + strand at 1646.844 kb on - strand at 1646.873 kb on - strand at 1646.975 kb on + strand at 1646.975 kb on + strand at 1646.976 kb on - strand at 1647.096 kb on + strand, within SM_b20626 at 1647.097 kb on - strand, within SM_b20626 at 1647.201 kb on + strand, within SM_b20626 at 1647.201 kb on + strand, within SM_b20626 at 1647.202 kb on - strand, within SM_b20626 at 1647.202 kb on - strand, within SM_b20626 at 1647.202 kb on - strand, within SM_b20626 at 1647.202 kb on - strand, within SM_b20626 at 1647.215 kb on + strand, within SM_b20626 at 1647.321 kb on + strand, within SM_b20626 at 1647.368 kb on - strand, within SM_b20626 at 1647.368 kb on - strand, within SM_b20626 at 1647.368 kb on - strand, within SM_b20626 at 1647.891 kb on + strand at 1647.891 kb on + strand at 1648.001 kb on - strand, within SM_b20627 at 1648.009 kb on - strand, within SM_b20627 at 1648.009 kb on - strand, within SM_b20627 at 1648.009 kb on - strand, within SM_b20627 at 1648.013 kb on - strand, within SM_b20627 at 1648.013 kb on - strand, within SM_b20627 at 1648.040 kb on - strand, within SM_b20627 at 1648.315 kb on + strand, within SM_b20628 at 1648.359 kb on + strand, within SM_b20628 at 1648.359 kb on + strand, within SM_b20628 at 1648.359 kb on + strand, within SM_b20628 at 1648.360 kb on - strand, within SM_b20628 at 1648.368 kb on - strand, within SM_b20628 at 1648.437 kb on - strand at 1648.437 kb on - strand, within SM_b20628 at 1648.437 kb on - strand, within SM_b20628 at 1648.462 kb on + strand at 1648.462 kb on + strand at 1648.463 kb on - strand at 1648.481 kb on - strand at 1648.481 kb on - strand at 1648.505 kb on + strand at 1648.505 kb on + strand at 1648.505 kb on + strand at 1648.600 kb on + strand, within SM_b20629 at 1648.622 kb on + strand, within SM_b20629 at 1648.623 kb on - strand, within SM_b20629 at 1648.623 kb on - strand, within SM_b20629 at 1648.746 kb on + strand, within SM_b20629 at 1648.746 kb on + strand, within SM_b20629 at 1648.763 kb on + strand, within SM_b20629 at 1648.808 kb on + strand, within SM_b20629
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,646,082 + SM_b20625 0.40 +1.7 1,646,082 + SM_b20625 0.40 -1.2 1,646,083 - SM_b20625 0.40 +0.8 1,646,083 - SM_b20625 0.40 -0.3 1,646,083 - SM_b20625 0.40 +0.5 1,646,083 - SM_b20625 0.40 -0.2 1,646,083 - SM_b20625 0.40 -0.9 1,646,297 - SM_b20625 0.57 +2.6 1,646,298 + SM_b20625 0.58 -0.6 1,646,307 - SM_b20625 0.58 +0.8 1,646,801 - +0.4 1,646,810 - +0.1 1,646,810 - +0.6 1,646,839 + -0.7 1,646,843 + -0.6 1,646,843 + -0.4 1,646,844 - -1.8 1,646,873 - +0.1 1,646,975 + +0.3 1,646,975 + -0.4 1,646,976 - -0.3 1,647,096 + SM_b20626 0.21 -0.4 1,647,097 - SM_b20626 0.21 -1.1 1,647,201 + SM_b20626 0.32 -0.7 1,647,201 + SM_b20626 0.32 +0.8 1,647,202 - SM_b20626 0.32 +0.2 1,647,202 - SM_b20626 0.32 -1.3 1,647,202 - SM_b20626 0.32 -0.9 1,647,202 - SM_b20626 0.32 -0.8 1,647,215 + SM_b20626 0.33 +0.0 1,647,321 + SM_b20626 0.44 -0.1 1,647,368 - SM_b20626 0.49 +0.4 1,647,368 - SM_b20626 0.49 -0.7 1,647,368 - SM_b20626 0.49 -2.9 1,647,891 + -1.0 1,647,891 + -0.1 1,648,001 - SM_b20627 0.31 +0.2 1,648,009 - SM_b20627 0.33 +0.8 1,648,009 - SM_b20627 0.33 -1.9 1,648,009 - SM_b20627 0.33 +0.1 1,648,013 - SM_b20627 0.35 +0.4 1,648,013 - SM_b20627 0.35 -0.1 1,648,040 - SM_b20627 0.43 -0.7 1,648,315 + SM_b20628 0.39 +0.5 1,648,359 + SM_b20628 0.55 -1.9 1,648,359 + SM_b20628 0.55 +1.1 1,648,359 + SM_b20628 0.55 +1.2 1,648,360 - SM_b20628 0.55 -0.5 1,648,368 - SM_b20628 0.58 -2.7 1,648,437 - -0.0 1,648,437 - SM_b20628 0.84 -0.2 1,648,437 - SM_b20628 0.84 -2.4 1,648,462 + +0.0 1,648,462 + +1.0 1,648,463 - -0.3 1,648,481 - -0.6 1,648,481 - -0.9 1,648,505 + +1.5 1,648,505 + -0.4 1,648,505 + +1.0 1,648,600 + SM_b20629 0.12 +0.4 1,648,622 + SM_b20629 0.20 +0.6 1,648,623 - SM_b20629 0.20 +0.4 1,648,623 - SM_b20629 0.20 -0.3 1,648,746 + SM_b20629 0.65 +0.4 1,648,746 + SM_b20629 0.65 -0.7 1,648,763 + SM_b20629 0.71 -1.0 1,648,808 + SM_b20629 0.87 +0.5
Or see this region's nucleotide sequence