Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20020 and SM_b20021 are separated by 116 nucleotides SM_b20021 and SM_b20022 are separated by 647 nucleotides
SM_b20020: SM_b20020 - pyruvate dehydrogenase E1 component, subunits alpha and beta, at 27,345 to 29,423
_b20020
SM_b20021: SM_b20021 - transcriptional regulator, at 29,540 to 30,010
_b20021
SM_b20022: SM_b20022 - hypothetical protein, at 30,658 to 31,224
_b20022
Position (kb)
29
30
31 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 28.597 kb on + strand, within SM_b20020 at 28.597 kb on + strand, within SM_b20020 at 28.781 kb on + strand, within SM_b20020 at 28.782 kb on - strand, within SM_b20020 at 28.843 kb on + strand, within SM_b20020 at 28.844 kb on - strand, within SM_b20020 at 28.844 kb on - strand, within SM_b20020 at 28.844 kb on - strand, within SM_b20020 at 28.982 kb on - strand, within SM_b20020 at 29.016 kb on - strand, within SM_b20020 at 29.016 kb on - strand, within SM_b20020 at 29.017 kb on + strand, within SM_b20020 at 29.194 kb on + strand, within SM_b20020 at 29.278 kb on + strand at 29.540 kb on - strand at 29.584 kb on - strand at 29.843 kb on + strand, within SM_b20021 at 29.987 kb on + strand at 29.987 kb on + strand at 29.988 kb on - strand at 30.008 kb on + strand at 30.170 kb on - strand at 30.170 kb on - strand at 30.189 kb on + strand at 30.189 kb on + strand at 30.189 kb on + strand at 30.190 kb on - strand at 30.196 kb on + strand at 30.196 kb on + strand at 30.197 kb on - strand at 30.370 kb on + strand at 30.371 kb on - strand at 30.371 kb on - strand at 30.471 kb on + strand at 30.471 kb on + strand at 30.471 kb on + strand at 30.471 kb on + strand at 30.471 kb on + strand at 30.472 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 28,597 + SM_b20020 0.60 -0.4 28,597 + SM_b20020 0.60 +0.1 28,781 + SM_b20020 0.69 -0.7 28,782 - SM_b20020 0.69 -0.8 28,843 + SM_b20020 0.72 -1.7 28,844 - SM_b20020 0.72 +0.5 28,844 - SM_b20020 0.72 +0.0 28,844 - SM_b20020 0.72 +0.4 28,982 - SM_b20020 0.79 -1.3 29,016 - SM_b20020 0.80 -1.0 29,016 - SM_b20020 0.80 +1.2 29,017 + SM_b20020 0.80 +1.5 29,194 + SM_b20020 0.89 +0.8 29,278 + +0.3 29,540 - +0.1 29,584 - +1.8 29,843 + SM_b20021 0.64 -1.7 29,987 + +1.6 29,987 + +0.7 29,988 - -2.1 30,008 + -0.8 30,170 - -0.8 30,170 - +0.0 30,189 + +0.1 30,189 + +0.7 30,189 + -0.7 30,190 - +0.4 30,196 + -0.0 30,196 + -1.1 30,197 - +0.3 30,370 + +0.8 30,371 - +0.8 30,371 - +2.5 30,471 + +0.9 30,471 + +0.2 30,471 + -0.9 30,471 + +0.9 30,471 + +0.5 30,472 - +1.6
Or see this region's nucleotide sequence