Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02240 and HSERO_RS02245 are separated by 185 nucleotides HSERO_RS02245 and HSERO_RS02250 are separated by 130 nucleotides HSERO_RS02250 and HSERO_RS02255 are separated by 207 nucleotides HSERO_RS02255 and HSERO_RS02260 are separated by 0 nucleotides
HSERO_RS02240: HSERO_RS02240 - homoserine kinase, at 489,761 to 490,717
_RS02240
HSERO_RS02245: HSERO_RS02245 - hypothetical protein, at 490,903 to 491,343
_RS02245
HSERO_RS02250: HSERO_RS02250 - hypothetical protein, at 491,474 to 492,166
_RS02250
HSERO_RS02255: HSERO_RS02255 - decarboxylase, at 492,374 to 493,120
_RS02255
HSERO_RS02260: HSERO_RS02260 - DNA polymerase I, at 493,121 to 495,883
_RS02260
Position (kb) 491
492
493 Strain fitness (log2 ratio) -2
-1
0
1 at 490.909 kb on - strand at 491.043 kb on - strand, within HSERO_RS02245 at 491.075 kb on - strand, within HSERO_RS02245 at 491.095 kb on - strand, within HSERO_RS02245 at 491.326 kb on + strand at 491.392 kb on + strand at 491.446 kb on - strand at 491.495 kb on + strand at 491.503 kb on - strand at 491.713 kb on + strand, within HSERO_RS02250 at 491.850 kb on - strand, within HSERO_RS02250 at 491.945 kb on - strand, within HSERO_RS02250 at 491.960 kb on + strand, within HSERO_RS02250 at 492.018 kb on + strand, within HSERO_RS02250 at 492.123 kb on + strand at 492.124 kb on + strand at 492.175 kb on + strand at 492.270 kb on + strand at 492.270 kb on + strand at 492.370 kb on + strand at 492.390 kb on - strand at 492.395 kb on - strand at 492.480 kb on + strand, within HSERO_RS02255 at 492.564 kb on + strand, within HSERO_RS02255 at 492.855 kb on - strand, within HSERO_RS02255 at 492.987 kb on - strand, within HSERO_RS02255
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 490,909 - -0.1 491,043 - HSERO_RS02245 0.32 +0.4 491,075 - HSERO_RS02245 0.39 +0.1 491,095 - HSERO_RS02245 0.44 -1.1 491,326 + -0.6 491,392 + +0.2 491,446 - -1.4 491,495 + -1.2 491,503 - -0.3 491,713 + HSERO_RS02250 0.34 +0.8 491,850 - HSERO_RS02250 0.54 +1.1 491,945 - HSERO_RS02250 0.68 +0.8 491,960 + HSERO_RS02250 0.70 +0.3 492,018 + HSERO_RS02250 0.78 +0.1 492,123 + +1.2 492,124 + -0.4 492,175 + -1.1 492,270 + -0.3 492,270 + +0.2 492,370 + +0.2 492,390 - +0.7 492,395 - +0.1 492,480 + HSERO_RS02255 0.14 -2.0 492,564 + HSERO_RS02255 0.25 -2.2 492,855 - HSERO_RS02255 0.64 +0.9 492,987 - HSERO_RS02255 0.82 +0.5
Or see this region's nucleotide sequence