Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS06545 and BPHYT_RS06550 are separated by 111 nucleotides BPHYT_RS06550 and BPHYT_RS06555 are separated by 119 nucleotides BPHYT_RS06555 and BPHYT_RS06560 are separated by 130 nucleotides BPHYT_RS06560 and BPHYT_RS06565 are separated by 24 nucleotides
BPHYT_RS06545: BPHYT_RS06545 - acetolactate synthase, at 1,485,407 to 1,487,170
_RS06545
BPHYT_RS06550: BPHYT_RS06550 - acetolactate synthase, at 1,487,282 to 1,487,773
_RS06550
BPHYT_RS06555: BPHYT_RS06555 - ketol-acid reductoisomerase, at 1,487,893 to 1,488,909
_RS06555
BPHYT_RS06560: BPHYT_RS06560 - phosphatidylserine decarboxylase proenzyme, at 1,489,040 to 1,489,678
_RS06560
BPHYT_RS06565: BPHYT_RS06565 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 1,489,703 to 1,490,569
_RS06565
Position (kb)
1487
1488
1489 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1486.931 kb on + strand, within BPHYT_RS06545 at 1486.943 kb on + strand, within BPHYT_RS06545 at 1486.943 kb on + strand, within BPHYT_RS06545 at 1486.943 kb on + strand, within BPHYT_RS06545 at 1486.943 kb on + strand, within BPHYT_RS06545 at 1487.166 kb on + strand at 1487.363 kb on + strand, within BPHYT_RS06550 at 1487.599 kb on + strand, within BPHYT_RS06550 at 1487.701 kb on + strand, within BPHYT_RS06550 at 1487.818 kb on + strand at 1487.840 kb on + strand at 1487.840 kb on + strand at 1487.840 kb on + strand at 1487.840 kb on + strand at 1487.840 kb on + strand at 1487.841 kb on - strand at 1487.841 kb on - strand at 1487.841 kb on - strand at 1487.841 kb on - strand at 1487.841 kb on - strand at 1487.842 kb on + strand at 1487.842 kb on + strand at 1487.842 kb on + strand at 1487.842 kb on + strand at 1487.842 kb on + strand at 1487.843 kb on - strand at 1487.843 kb on - strand at 1488.157 kb on + strand, within BPHYT_RS06555 at 1488.158 kb on - strand, within BPHYT_RS06555 at 1488.229 kb on + strand, within BPHYT_RS06555 at 1488.230 kb on - strand, within BPHYT_RS06555 at 1488.310 kb on + strand, within BPHYT_RS06555 at 1488.544 kb on + strand, within BPHYT_RS06555 at 1488.545 kb on - strand, within BPHYT_RS06555 at 1488.562 kb on + strand, within BPHYT_RS06555 at 1488.562 kb on + strand, within BPHYT_RS06555 at 1488.634 kb on + strand, within BPHYT_RS06555 at 1488.749 kb on - strand, within BPHYT_RS06555 at 1488.908 kb on - strand at 1488.908 kb on - strand at 1488.908 kb on - strand at 1488.947 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X remove 1,486,931 + BPHYT_RS06545 0.86 -3.0 1,486,943 + BPHYT_RS06545 0.87 -4.5 1,486,943 + BPHYT_RS06545 0.87 -2.1 1,486,943 + BPHYT_RS06545 0.87 -3.8 1,486,943 + BPHYT_RS06545 0.87 -4.3 1,487,166 + +0.5 1,487,363 + BPHYT_RS06550 0.16 -1.5 1,487,599 + BPHYT_RS06550 0.64 -0.4 1,487,701 + BPHYT_RS06550 0.85 -0.4 1,487,818 + -0.1 1,487,840 + +0.9 1,487,840 + +0.3 1,487,840 + -0.8 1,487,840 + +0.9 1,487,840 + -0.5 1,487,841 - -2.9 1,487,841 - -3.5 1,487,841 - -2.9 1,487,841 - -3.7 1,487,841 - -1.6 1,487,842 + +0.3 1,487,842 + -1.9 1,487,842 + -0.1 1,487,842 + +0.1 1,487,842 + -0.7 1,487,843 - -3.1 1,487,843 - -3.6 1,488,157 + BPHYT_RS06555 0.26 -4.1 1,488,158 - BPHYT_RS06555 0.26 -2.3 1,488,229 + BPHYT_RS06555 0.33 -5.6 1,488,230 - BPHYT_RS06555 0.33 -6.0 1,488,310 + BPHYT_RS06555 0.41 -2.5 1,488,544 + BPHYT_RS06555 0.64 -1.8 1,488,545 - BPHYT_RS06555 0.64 -4.2 1,488,562 + BPHYT_RS06555 0.66 -2.5 1,488,562 + BPHYT_RS06555 0.66 -6.0 1,488,634 + BPHYT_RS06555 0.73 -1.7 1,488,749 - BPHYT_RS06555 0.84 -4.0 1,488,908 - -0.8 1,488,908 - -0.7 1,488,908 - -1.5 1,488,947 + -0.5
Or see this region's nucleotide sequence