Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc04882 and SMc04383 are separated by 113 nucleotides SMc04383 and SMc04384 are separated by 10 nucleotides
SMc04882: SMc04882 - transcriptional regulator, at 3,582,240 to 3,583,133
SMc04882
SMc04383: SMc04383 - putative hydrolase, required for lysine catabolism (from data) , at 3,583,247 to 3,584,647
SMc04383
SMc04384: SMc04384 - (R)-2-hydroxyglutarate dehydrogenase (EC 1.1.99.39) (from data) , at 3,584,658 to 3,586,079
SMc04384
Position (kb)
3583
3584
3585 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3582.320 kb on - strand at 3582.320 kb on - strand at 3582.322 kb on + strand at 3582.322 kb on + strand at 3582.322 kb on + strand at 3582.323 kb on - strand at 3582.323 kb on - strand at 3582.323 kb on - strand at 3582.406 kb on - strand, within SMc04882 at 3582.594 kb on + strand, within SMc04882 at 3582.647 kb on - strand, within SMc04882 at 3582.647 kb on - strand, within SMc04882 at 3583.258 kb on + strand at 3583.258 kb on + strand at 3583.337 kb on + strand at 3583.527 kb on - strand, within SMc04383 at 3583.539 kb on - strand, within SMc04383 at 3583.539 kb on - strand, within SMc04383 at 3583.541 kb on + strand, within SMc04383 at 3583.541 kb on + strand, within SMc04383 at 3583.717 kb on + strand, within SMc04383 at 3583.853 kb on + strand, within SMc04383 at 3583.915 kb on + strand, within SMc04383 at 3584.164 kb on - strand, within SMc04383 at 3584.250 kb on - strand, within SMc04383 at 3584.433 kb on - strand, within SMc04383 at 3584.464 kb on + strand, within SMc04383 at 3584.576 kb on + strand at 3584.576 kb on + strand at 3584.623 kb on + strand at 3584.648 kb on + strand at 3584.873 kb on + strand, within SMc04384 at 3585.123 kb on + strand, within SMc04384 at 3585.501 kb on + strand, within SMc04384 at 3585.592 kb on + strand, within SMc04384 at 3585.593 kb on - strand, within SMc04384 at 3585.610 kb on + strand, within SMc04384 at 3585.610 kb on + strand, within SMc04384 at 3585.610 kb on + strand, within SMc04384
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,582,320 - +2.2 3,582,320 - +1.3 3,582,322 + +1.7 3,582,322 + +1.2 3,582,322 + +2.1 3,582,323 - +0.0 3,582,323 - -2.5 3,582,323 - -0.4 3,582,406 - SMc04882 0.19 -3.7 3,582,594 + SMc04882 0.40 -0.4 3,582,647 - SMc04882 0.46 +0.3 3,582,647 - SMc04882 0.46 -0.2 3,583,258 + +2.4 3,583,258 + +0.2 3,583,337 + -0.6 3,583,527 - SMc04383 0.20 -0.2 3,583,539 - SMc04383 0.21 -1.4 3,583,539 - SMc04383 0.21 -2.2 3,583,541 + SMc04383 0.21 -2.4 3,583,541 + SMc04383 0.21 -1.9 3,583,717 + SMc04383 0.34 -2.5 3,583,853 + SMc04383 0.43 -1.5 3,583,915 + SMc04383 0.48 -2.7 3,584,164 - SMc04383 0.65 +2.2 3,584,250 - SMc04383 0.72 -2.1 3,584,433 - SMc04383 0.85 -1.2 3,584,464 + SMc04383 0.87 +1.2 3,584,576 + -1.0 3,584,576 + +1.0 3,584,623 + -1.5 3,584,648 + -0.6 3,584,873 + SMc04384 0.15 +0.4 3,585,123 + SMc04384 0.33 -0.2 3,585,501 + SMc04384 0.59 -1.5 3,585,592 + SMc04384 0.66 +1.0 3,585,593 - SMc04384 0.66 -2.3 3,585,610 + SMc04384 0.67 -0.6 3,585,610 + SMc04384 0.67 -0.3 3,585,610 + SMc04384 0.67 -3.7
Or see this region's nucleotide sequence