Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc04450 and SMc00227 are separated by 2 nucleotides SMc00227 and SMc00228 are separated by 116 nucleotides SMc00228 and SMc00229 are separated by 14 nucleotides SMc00229 and SMc00230 are separated by 18 nucleotides
SMc04450: SMc04450 - transcription-repair coupling factor, at 1,787,537 to 1,791,052
SMc04450
SMc00227: SMc00227 - hypothetical protein, at 1,791,055 to 1,791,360
SMc00227
SMc00228: SMc00228 - ATP-dependent DNA helicase RecG, at 1,791,477 to 1,793,582
SMc00228
SMc00229: SMc00229 - hypothetical protein, at 1,793,597 to 1,794,289
SMc00229
SMc00230: SMc00230 - hypothetical protein, at 1,794,308 to 1,794,805
SMc00230
Position (kb)
1791
1792
1793
1794 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1790.516 kb on + strand, within SMc04450 at 1790.517 kb on - strand, within SMc04450 at 1790.520 kb on + strand, within SMc04450 at 1790.521 kb on - strand, within SMc04450 at 1790.521 kb on - strand, within SMc04450 at 1790.521 kb on - strand, within SMc04450 at 1790.521 kb on - strand, within SMc04450 at 1790.521 kb on - strand, within SMc04450 at 1790.522 kb on - strand, within SMc04450 at 1790.919 kb on + strand at 1790.919 kb on + strand at 1790.920 kb on - strand at 1790.920 kb on - strand at 1790.920 kb on - strand at 1791.041 kb on + strand at 1791.042 kb on - strand at 1791.042 kb on - strand at 1791.042 kb on - strand at 1791.042 kb on - strand at 1791.042 kb on - strand at 1791.412 kb on - strand at 1791.414 kb on - strand at 1791.457 kb on - strand at 1791.457 kb on - strand at 1791.476 kb on + strand at 1791.477 kb on - strand at 1791.477 kb on - strand at 1791.618 kb on - strand at 1791.906 kb on + strand, within SMc00228 at 1791.906 kb on + strand, within SMc00228 at 1791.906 kb on + strand, within SMc00228 at 1791.906 kb on + strand, within SMc00228 at 1791.907 kb on - strand, within SMc00228 at 1791.907 kb on - strand, within SMc00228 at 1792.128 kb on + strand, within SMc00228 at 1792.132 kb on + strand, within SMc00228 at 1792.132 kb on + strand, within SMc00228 at 1792.132 kb on + strand, within SMc00228 at 1792.132 kb on + strand, within SMc00228 at 1792.133 kb on - strand, within SMc00228 at 1792.133 kb on - strand, within SMc00228 at 1792.133 kb on - strand, within SMc00228 at 1792.168 kb on - strand, within SMc00228 at 1792.580 kb on - strand, within SMc00228 at 1792.813 kb on + strand, within SMc00228 at 1792.935 kb on + strand, within SMc00228 at 1792.935 kb on + strand, within SMc00228 at 1792.936 kb on - strand, within SMc00228 at 1793.121 kb on - strand, within SMc00228 at 1793.265 kb on + strand, within SMc00228 at 1793.266 kb on - strand, within SMc00228 at 1793.266 kb on - strand, within SMc00228 at 1793.266 kb on - strand, within SMc00228 at 1793.473 kb on - strand at 1793.479 kb on + strand at 1793.479 kb on + strand at 1793.535 kb on + strand at 1793.536 kb on - strand at 1793.536 kb on - strand at 1793.964 kb on + strand, within SMc00229 at 1793.965 kb on - strand, within SMc00229 at 1794.114 kb on + strand, within SMc00229 at 1794.127 kb on + strand, within SMc00229 at 1794.128 kb on - strand, within SMc00229 at 1794.129 kb on + strand, within SMc00229 at 1794.145 kb on - strand, within SMc00229 at 1794.145 kb on - strand, within SMc00229 at 1794.241 kb on - strand at 1794.241 kb on - strand at 1794.241 kb on - strand at 1794.371 kb on + strand, within SMc00230 at 1794.384 kb on + strand, within SMc00230 at 1794.384 kb on + strand, within SMc00230 at 1794.444 kb on + strand, within SMc00230 at 1794.444 kb on + strand, within SMc00230 at 1794.445 kb on - strand at 1794.579 kb on + strand, within SMc00230
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,790,516 + SMc04450 0.85 +0.1 1,790,517 - SMc04450 0.85 -0.2 1,790,520 + SMc04450 0.85 +0.3 1,790,521 - SMc04450 0.85 +0.3 1,790,521 - SMc04450 0.85 +0.3 1,790,521 - SMc04450 0.85 -0.4 1,790,521 - SMc04450 0.85 -0.9 1,790,521 - SMc04450 0.85 -1.2 1,790,522 - SMc04450 0.85 +1.3 1,790,919 + +0.7 1,790,919 + +0.9 1,790,920 - -0.1 1,790,920 - +0.9 1,790,920 - -0.6 1,791,041 + +0.9 1,791,042 - -0.6 1,791,042 - +0.1 1,791,042 - +0.7 1,791,042 - +0.7 1,791,042 - +0.6 1,791,412 - -0.1 1,791,414 - +0.3 1,791,457 - -0.7 1,791,457 - +0.1 1,791,476 + -0.2 1,791,477 - -0.4 1,791,477 - +0.2 1,791,618 - +1.5 1,791,906 + SMc00228 0.20 -1.8 1,791,906 + SMc00228 0.20 -0.1 1,791,906 + SMc00228 0.20 -1.1 1,791,906 + SMc00228 0.20 -0.2 1,791,907 - SMc00228 0.20 -0.4 1,791,907 - SMc00228 0.20 -2.4 1,792,128 + SMc00228 0.31 -0.1 1,792,132 + SMc00228 0.31 -0.1 1,792,132 + SMc00228 0.31 -0.1 1,792,132 + SMc00228 0.31 -0.5 1,792,132 + SMc00228 0.31 -1.3 1,792,133 - SMc00228 0.31 -3.1 1,792,133 - SMc00228 0.31 -0.5 1,792,133 - SMc00228 0.31 +0.6 1,792,168 - SMc00228 0.33 -0.3 1,792,580 - SMc00228 0.52 -2.0 1,792,813 + SMc00228 0.63 +0.0 1,792,935 + SMc00228 0.69 -0.0 1,792,935 + SMc00228 0.69 -1.8 1,792,936 - SMc00228 0.69 +0.9 1,793,121 - SMc00228 0.78 -0.1 1,793,265 + SMc00228 0.85 -2.9 1,793,266 - SMc00228 0.85 -1.9 1,793,266 - SMc00228 0.85 -1.6 1,793,266 - SMc00228 0.85 +2.9 1,793,473 - -1.7 1,793,479 + -1.3 1,793,479 + -1.2 1,793,535 + -0.5 1,793,536 - -0.7 1,793,536 - +0.6 1,793,964 + SMc00229 0.53 -0.9 1,793,965 - SMc00229 0.53 -1.8 1,794,114 + SMc00229 0.75 -0.5 1,794,127 + SMc00229 0.76 -1.8 1,794,128 - SMc00229 0.77 +0.9 1,794,129 + SMc00229 0.77 -0.3 1,794,145 - SMc00229 0.79 +0.2 1,794,145 - SMc00229 0.79 -0.5 1,794,241 - -1.0 1,794,241 - +1.2 1,794,241 - -0.2 1,794,371 + SMc00230 0.13 +0.7 1,794,384 + SMc00230 0.15 -1.7 1,794,384 + SMc00230 0.15 +1.2 1,794,444 + SMc00230 0.27 +2.2 1,794,444 + SMc00230 0.27 -2.0 1,794,445 - -2.1 1,794,579 + SMc00230 0.54 -2.5
Or see this region's nucleotide sequence