Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1239 and SMa1240 overlap by 8 nucleotides SMa1240 and SMa1241 are separated by 7 nucleotides SMa1241 and SMa1243 are separated by 259 nucleotides SMa1243 and SMa1245 are separated by 110 nucleotides
SMa1239: SMa1239 - NapD component of periplasmic nitrate reductase, at 679,677 to 679,964
SMa1239
SMa1240: SMa1240 - NapF ferredoxin component of periplasmic nitrate reductase, at 679,957 to 680,457
SMa1240
SMa1241: SMa1241 - NapE component of periplasmic nitrate reductase, at 680,465 to 680,650
SMa1241
SMa1243: SMa1243 - Azu1 pseudoazurin, at 680,910 to 681,353
SMa1243
SMa1245: SMa1245 - Crp/fnr-like transcriptional regulator, at 681,464 to 682,111
SMa1245
Position (kb) 680
681
682 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 679.937 kb on + strand at 679.942 kb on + strand at 679.942 kb on + strand at 679.942 kb on + strand at 680.055 kb on + strand, within SMa1240 at 680.731 kb on - strand at 680.911 kb on + strand at 681.092 kb on + strand at 681.094 kb on + strand, within SMa1243 at 681.094 kb on + strand, within SMa1243 at 681.095 kb on - strand, within SMa1243 at 681.164 kb on + strand, within SMa1243 at 681.164 kb on + strand, within SMa1243 at 681.165 kb on - strand, within SMa1243 at 681.165 kb on - strand, within SMa1243 at 681.406 kb on - strand at 681.411 kb on - strand at 681.465 kb on + strand at 681.466 kb on - strand at 681.644 kb on + strand, within SMa1245 at 681.645 kb on - strand, within SMa1245 at 681.784 kb on + strand, within SMa1245 at 681.784 kb on + strand, within SMa1245 at 681.784 kb on + strand, within SMa1245 at 681.785 kb on - strand, within SMa1245 at 682.171 kb on + strand at 682.217 kb on + strand at 682.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 679,937 + -0.9 679,942 + +0.1 679,942 + -0.6 679,942 + -0.9 680,055 + SMa1240 0.20 -2.3 680,731 - +1.7 680,911 + -0.7 681,092 + +0.7 681,094 + SMa1243 0.41 +0.3 681,094 + SMa1243 0.41 -1.8 681,095 - SMa1243 0.42 -0.7 681,164 + SMa1243 0.57 +0.6 681,164 + SMa1243 0.57 -0.9 681,165 - SMa1243 0.57 -3.7 681,165 - SMa1243 0.57 -0.4 681,406 - -0.1 681,411 - +0.4 681,465 + -2.5 681,466 - +0.1 681,644 + SMa1245 0.28 -2.6 681,645 - SMa1245 0.28 +0.0 681,784 + SMa1245 0.49 -4.1 681,784 + SMa1245 0.49 +0.9 681,784 + SMa1245 0.49 +2.5 681,785 - SMa1245 0.50 +2.6 682,171 + +0.8 682,217 + -3.1 682,218 - -2.5
Or see this region's nucleotide sequence