Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0228 and SMa0229 are separated by 318 nucleotides SMa0229 and SMa0232 are separated by 219 nucleotides SMa0232 and SMa0233 are separated by 21 nucleotides
SMa0228: SMa0228 - glutamate dehydrogenase, at 126,420 to 127,766
SMa0228
SMa0229: SMa0229 - hypothetical protein, at 128,085 to 128,507
SMa0229
SMa0232: SMa0232 - hypothetical protein, at 128,727 to 129,278
SMa0232
SMa0233: SMa0233 - OtsA trehalose-6-phosphate synthase, at 129,300 to 130,691
SMa0233
Position (kb) 128
129
130 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 127.896 kb on + strand at 127.896 kb on + strand at 127.896 kb on + strand at 127.896 kb on + strand at 127.896 kb on + strand at 127.897 kb on - strand at 127.898 kb on + strand at 127.899 kb on - strand at 127.941 kb on - strand at 128.030 kb on - strand at 128.074 kb on + strand at 128.075 kb on - strand at 128.075 kb on - strand at 128.075 kb on - strand at 128.075 kb on - strand at 128.142 kb on + strand, within SMa0229 at 128.166 kb on + strand, within SMa0229 at 128.166 kb on + strand, within SMa0229 at 128.279 kb on + strand, within SMa0229 at 128.279 kb on + strand, within SMa0229 at 128.279 kb on + strand, within SMa0229 at 128.279 kb on + strand, within SMa0229 at 128.280 kb on - strand, within SMa0229 at 128.280 kb on - strand, within SMa0229 at 128.332 kb on - strand, within SMa0229 at 128.332 kb on - strand, within SMa0229 at 128.462 kb on - strand, within SMa0229 at 128.462 kb on - strand, within SMa0229 at 128.607 kb on - strand at 128.704 kb on + strand at 128.705 kb on - strand at 128.902 kb on + strand, within SMa0232 at 128.902 kb on + strand, within SMa0232 at 128.903 kb on - strand, within SMa0232 at 128.914 kb on + strand, within SMa0232 at 128.915 kb on - strand, within SMa0232 at 128.997 kb on - strand, within SMa0232 at 129.301 kb on + strand at 129.389 kb on + strand at 129.390 kb on - strand at 129.428 kb on + strand at 129.428 kb on + strand at 129.444 kb on - strand, within SMa0233 at 129.444 kb on - strand, within SMa0233 at 129.465 kb on - strand, within SMa0233 at 129.465 kb on - strand, within SMa0233 at 129.469 kb on + strand, within SMa0233 at 129.469 kb on + strand, within SMa0233 at 129.496 kb on - strand, within SMa0233 at 129.569 kb on - strand, within SMa0233 at 129.569 kb on - strand, within SMa0233 at 129.672 kb on - strand, within SMa0233 at 129.672 kb on - strand, within SMa0233 at 129.674 kb on + strand, within SMa0233 at 129.674 kb on + strand, within SMa0233 at 129.674 kb on + strand, within SMa0233 at 129.675 kb on - strand, within SMa0233 at 129.677 kb on - strand, within SMa0233 at 129.677 kb on - strand, within SMa0233 at 129.677 kb on - strand, within SMa0233 at 129.677 kb on - strand, within SMa0233 at 129.681 kb on + strand, within SMa0233 at 129.682 kb on - strand, within SMa0233 at 129.682 kb on - strand at 129.682 kb on - strand, within SMa0233 at 129.682 kb on - strand, within SMa0233 at 129.803 kb on - strand, within SMa0233 at 129.927 kb on - strand, within SMa0233 at 129.999 kb on + strand, within SMa0233 at 130.012 kb on + strand, within SMa0233 at 130.013 kb on - strand, within SMa0233 at 130.013 kb on - strand, within SMa0233 at 130.013 kb on - strand, within SMa0233 at 130.013 kb on - strand, within SMa0233 at 130.013 kb on - strand, within SMa0233 at 130.013 kb on - strand, within SMa0233 at 130.039 kb on + strand, within SMa0233 at 130.040 kb on - strand, within SMa0233 at 130.144 kb on + strand, within SMa0233 at 130.194 kb on + strand, within SMa0233
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 127,896 + +2.4 127,896 + -0.3 127,896 + +0.9 127,896 + -0.0 127,896 + -0.8 127,897 - +0.5 127,898 + -1.1 127,899 - +1.1 127,941 - -0.9 128,030 - -0.3 128,074 + -0.6 128,075 - +0.4 128,075 - +1.2 128,075 - -3.1 128,075 - +2.1 128,142 + SMa0229 0.13 +0.2 128,166 + SMa0229 0.19 +0.7 128,166 + SMa0229 0.19 +1.3 128,279 + SMa0229 0.46 -1.6 128,279 + SMa0229 0.46 -1.8 128,279 + SMa0229 0.46 -1.4 128,279 + SMa0229 0.46 -2.5 128,280 - SMa0229 0.46 -2.9 128,280 - SMa0229 0.46 -0.6 128,332 - SMa0229 0.58 -0.1 128,332 - SMa0229 0.58 -0.6 128,462 - SMa0229 0.89 +0.4 128,462 - SMa0229 0.89 -0.2 128,607 - -0.4 128,704 + +0.3 128,705 - +1.1 128,902 + SMa0232 0.32 -0.8 128,902 + SMa0232 0.32 -3.2 128,903 - SMa0232 0.32 -0.6 128,914 + SMa0232 0.34 -1.6 128,915 - SMa0232 0.34 -4.4 128,997 - SMa0232 0.49 -0.7 129,301 + +1.7 129,389 + -1.4 129,390 - +0.1 129,428 + +0.2 129,428 + +2.1 129,444 - SMa0233 0.10 -0.1 129,444 - SMa0233 0.10 -3.8 129,465 - SMa0233 0.12 -1.4 129,465 - SMa0233 0.12 -1.2 129,469 + SMa0233 0.12 +0.9 129,469 + SMa0233 0.12 +0.5 129,496 - SMa0233 0.14 -1.3 129,569 - SMa0233 0.19 -0.9 129,569 - SMa0233 0.19 -0.2 129,672 - SMa0233 0.27 -1.0 129,672 - SMa0233 0.27 +0.9 129,674 + SMa0233 0.27 +0.4 129,674 + SMa0233 0.27 -1.0 129,674 + SMa0233 0.27 +0.8 129,675 - SMa0233 0.27 +0.4 129,677 - SMa0233 0.27 +0.0 129,677 - SMa0233 0.27 +0.7 129,677 - SMa0233 0.27 -0.0 129,677 - SMa0233 0.27 -0.1 129,681 + SMa0233 0.27 -0.3 129,682 - SMa0233 0.27 -0.8 129,682 - -0.1 129,682 - SMa0233 0.27 +0.6 129,682 - SMa0233 0.27 -0.1 129,803 - SMa0233 0.36 +1.4 129,927 - SMa0233 0.45 +0.4 129,999 + SMa0233 0.50 +0.3 130,012 + SMa0233 0.51 -1.5 130,013 - SMa0233 0.51 -0.1 130,013 - SMa0233 0.51 +1.8 130,013 - SMa0233 0.51 +1.0 130,013 - SMa0233 0.51 +0.8 130,013 - SMa0233 0.51 +1.2 130,013 - SMa0233 0.51 -0.1 130,039 + SMa0233 0.53 -0.8 130,040 - SMa0233 0.53 -0.2 130,144 + SMa0233 0.61 +0.3 130,194 + SMa0233 0.64 -1.6
Or see this region's nucleotide sequence