Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20814 and SM_b20815 are separated by 50 nucleotides SM_b20815 and SM_b20816 are separated by 65 nucleotides
SM_b20814: SM_b20814 - hypothetical protein, at 601,995 to 602,735
_b20814
SM_b20815: SM_b20815 - outer membrane protein assembly protein, at 602,786 to 604,453
_b20815
SM_b20816: SM_b20816 - hypothetical protein, at 604,519 to 605,808
_b20816
Position (kb) 602
603
604
605 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 601.839 kb on + strand at 601.840 kb on - strand at 601.898 kb on + strand at 601.898 kb on + strand at 601.898 kb on + strand at 601.899 kb on - strand at 601.899 kb on - strand at 602.000 kb on + strand at 602.000 kb on + strand at 602.001 kb on - strand at 602.001 kb on - strand at 602.002 kb on + strand at 602.002 kb on + strand at 602.002 kb on + strand at 602.016 kb on - strand at 602.165 kb on + strand, within SM_b20814 at 602.166 kb on - strand, within SM_b20814 at 602.166 kb on - strand, within SM_b20814 at 602.166 kb on - strand, within SM_b20814 at 602.166 kb on - strand, within SM_b20814 at 602.166 kb on - strand, within SM_b20814 at 602.189 kb on + strand, within SM_b20814 at 602.332 kb on - strand, within SM_b20814 at 602.471 kb on + strand, within SM_b20814 at 602.471 kb on + strand, within SM_b20814 at 602.496 kb on + strand, within SM_b20814 at 602.560 kb on + strand, within SM_b20814 at 602.560 kb on + strand, within SM_b20814 at 602.561 kb on - strand, within SM_b20814 at 602.569 kb on + strand, within SM_b20814 at 602.569 kb on + strand, within SM_b20814 at 602.570 kb on - strand, within SM_b20814 at 602.652 kb on + strand, within SM_b20814 at 602.656 kb on - strand, within SM_b20814 at 602.656 kb on - strand, within SM_b20814 at 602.718 kb on + strand at 602.718 kb on + strand at 602.718 kb on + strand at 602.734 kb on - strand at 602.739 kb on + strand at 602.916 kb on - strand at 603.008 kb on + strand, within SM_b20815 at 603.172 kb on - strand, within SM_b20815 at 603.525 kb on - strand, within SM_b20815 at 603.744 kb on + strand, within SM_b20815 at 603.744 kb on + strand, within SM_b20815 at 604.058 kb on - strand, within SM_b20815 at 604.068 kb on - strand, within SM_b20815 at 604.068 kb on - strand, within SM_b20815 at 604.215 kb on + strand, within SM_b20815 at 604.216 kb on - strand, within SM_b20815 at 604.482 kb on + strand at 604.520 kb on + strand at 604.520 kb on + strand at 604.521 kb on - strand at 604.521 kb on - strand at 605.053 kb on + strand, within SM_b20816 at 605.053 kb on + strand, within SM_b20816 at 605.061 kb on - strand, within SM_b20816 at 605.200 kb on - strand, within SM_b20816 at 605.333 kb on - strand, within SM_b20816 at 605.333 kb on - strand, within SM_b20816
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 601,839 + -0.2 601,840 - -3.0 601,898 + -0.6 601,898 + +2.5 601,898 + -0.8 601,899 - +0.3 601,899 - +0.9 602,000 + +0.6 602,000 + +1.4 602,001 - -0.3 602,001 - +2.9 602,002 + +1.0 602,002 + +0.3 602,002 + -0.2 602,016 - -0.7 602,165 + SM_b20814 0.23 +0.6 602,166 - SM_b20814 0.23 +0.3 602,166 - SM_b20814 0.23 -0.9 602,166 - SM_b20814 0.23 +0.0 602,166 - SM_b20814 0.23 +1.3 602,166 - SM_b20814 0.23 +0.5 602,189 + SM_b20814 0.26 -0.1 602,332 - SM_b20814 0.45 -1.0 602,471 + SM_b20814 0.64 +0.5 602,471 + SM_b20814 0.64 -2.9 602,496 + SM_b20814 0.68 -0.4 602,560 + SM_b20814 0.76 -0.8 602,560 + SM_b20814 0.76 +0.1 602,561 - SM_b20814 0.76 +0.6 602,569 + SM_b20814 0.77 -2.0 602,569 + SM_b20814 0.77 -0.5 602,570 - SM_b20814 0.78 +0.9 602,652 + SM_b20814 0.89 +1.9 602,656 - SM_b20814 0.89 +0.2 602,656 - SM_b20814 0.89 -1.5 602,718 + -1.5 602,718 + -1.7 602,718 + +1.2 602,734 - -0.1 602,739 + -0.2 602,916 - +1.3 603,008 + SM_b20815 0.13 +0.3 603,172 - SM_b20815 0.23 +1.5 603,525 - SM_b20815 0.44 +1.1 603,744 + SM_b20815 0.57 -1.2 603,744 + SM_b20815 0.57 +1.2 604,058 - SM_b20815 0.76 +2.2 604,068 - SM_b20815 0.77 -3.8 604,068 - SM_b20815 0.77 +0.7 604,215 + SM_b20815 0.86 -1.3 604,216 - SM_b20815 0.86 +1.9 604,482 + -0.6 604,520 + +1.5 604,520 + -1.2 604,521 - +0.0 604,521 - -1.8 605,053 + SM_b20816 0.41 +0.3 605,053 + SM_b20816 0.41 -0.3 605,061 - SM_b20816 0.42 -0.5 605,200 - SM_b20816 0.53 -1.9 605,333 - SM_b20816 0.63 -0.4 605,333 - SM_b20816 0.63 +0.2
Or see this region's nucleotide sequence