Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc04023 and SMc04024 are separated by 302 nucleotides SMc04024 and SMc04025 are separated by 102 nucleotides
SMc04023: SMc04023 - UTP--glucose-1-phosphate uridylyltransferase, at 3,021,043 to 3,021,930
SMc04023
SMc04024: SMc04024 - membrane-bound lytic murein transglycosylase, at 3,022,233 to 3,023,450
SMc04024
SMc04025: SMc04025 - hypothetical protein, at 3,023,553 to 3,024,815
SMc04025
Position (kb)
3022
3023
3024 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3021.299 kb on + strand, within SMc04023 at 3021.300 kb on - strand, within SMc04023 at 3021.414 kb on + strand, within SMc04023 at 3021.414 kb on + strand, within SMc04023 at 3021.415 kb on - strand, within SMc04023 at 3021.723 kb on - strand, within SMc04023 at 3021.803 kb on + strand, within SMc04023 at 3021.803 kb on + strand, within SMc04023 at 3021.803 kb on + strand, within SMc04023 at 3021.803 kb on + strand, within SMc04023 at 3021.803 kb on + strand, within SMc04023 at 3021.804 kb on - strand, within SMc04023 at 3021.804 kb on - strand, within SMc04023 at 3021.975 kb on - strand at 3021.975 kb on - strand at 3022.113 kb on + strand at 3022.113 kb on + strand at 3022.354 kb on - strand at 3022.453 kb on - strand, within SMc04024 at 3022.485 kb on + strand, within SMc04024 at 3022.486 kb on - strand, within SMc04024 at 3022.510 kb on - strand, within SMc04024 at 3022.666 kb on - strand, within SMc04024 at 3022.695 kb on + strand, within SMc04024 at 3022.695 kb on + strand, within SMc04024 at 3022.840 kb on - strand, within SMc04024 at 3022.840 kb on - strand, within SMc04024 at 3022.884 kb on - strand, within SMc04024 at 3023.002 kb on - strand, within SMc04024 at 3023.166 kb on + strand, within SMc04024 at 3023.417 kb on + strand at 3023.418 kb on - strand at 3023.449 kb on - strand at 3023.572 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,021,299 + SMc04023 0.29 +1.6 3,021,300 - SMc04023 0.29 -0.9 3,021,414 + SMc04023 0.42 +1.6 3,021,414 + SMc04023 0.42 -0.6 3,021,415 - SMc04023 0.42 +0.6 3,021,723 - SMc04023 0.77 -0.0 3,021,803 + SMc04023 0.86 -0.8 3,021,803 + SMc04023 0.86 +1.6 3,021,803 + SMc04023 0.86 -0.9 3,021,803 + SMc04023 0.86 -0.3 3,021,803 + SMc04023 0.86 +1.3 3,021,804 - SMc04023 0.86 -0.7 3,021,804 - SMc04023 0.86 +0.2 3,021,975 - -0.3 3,021,975 - -0.1 3,022,113 + -0.3 3,022,113 + -0.6 3,022,354 - -3.1 3,022,453 - SMc04024 0.18 -3.7 3,022,485 + SMc04024 0.21 -0.3 3,022,486 - SMc04024 0.21 -0.6 3,022,510 - SMc04024 0.23 -1.3 3,022,666 - SMc04024 0.36 -3.3 3,022,695 + SMc04024 0.38 -2.5 3,022,695 + SMc04024 0.38 -2.0 3,022,840 - SMc04024 0.50 -1.3 3,022,840 - SMc04024 0.50 -1.3 3,022,884 - SMc04024 0.53 -2.6 3,023,002 - SMc04024 0.63 -2.5 3,023,166 + SMc04024 0.77 -4.3 3,023,417 + -2.7 3,023,418 - -2.4 3,023,449 - -0.9 3,023,572 + -1.3
Or see this region's nucleotide sequence