Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00466 and SMc00548 are separated by 134 nucleotides SMc00548 and SMc00465 are separated by 39 nucleotides SMc00465 and SMc00463 overlap by 14 nucleotides SMc00463 and SMc00462 are separated by 14 nucleotides
SMc00466: SMc00466 - hypothetical protein, at 1,959,117 to 1,960,196
SMc00466
SMc00548: SMc00548 - signal peptide protein, at 1,960,331 to 1,960,882
SMc00548
SMc00465: SMc00465 - 7, 8-dihydro-6-hydroxymethylpterin-pyrophosphokinase, at 1,960,922 to 1,961,446
SMc00465
SMc00463: SMc00463 - dihydroneopterin aldolase DHNA lyase folate biosynthesis protein, at 1,961,433 to 1,961,804
SMc00463
SMc00462: SMc00462 - dihydropteroate synthase antibiotic resistance transferase folate protein, at 1,961,819 to 1,962,670
SMc00462
Position (kb)
1960
1961 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1959.450 kb on + strand, within SMc00466 at 1959.846 kb on + strand, within SMc00466 at 1959.847 kb on - strand, within SMc00466 at 1959.886 kb on - strand, within SMc00466 at 1959.886 kb on - strand, within SMc00466 at 1960.880 kb on + strand at 1960.923 kb on + strand at 1960.923 kb on + strand at 1961.843 kb on - strand at 1961.843 kb on - strand at 1961.843 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,959,450 + SMc00466 0.31 +0.3 1,959,846 + SMc00466 0.68 -1.6 1,959,847 - SMc00466 0.68 -4.0 1,959,886 - SMc00466 0.71 -1.9 1,959,886 - SMc00466 0.71 -0.1 1,960,880 + +0.5 1,960,923 + -1.2 1,960,923 + -0.7 1,961,843 - -1.1 1,961,843 - +0.1 1,961,843 - -0.5
Or see this region's nucleotide sequence