Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1211 and SMa1213 are separated by 228 nucleotides SMa1213 and SMa1214 are separated by 3 nucleotides SMa1214 and SMa1216 are separated by 12 nucleotides SMa1216 and SMa1220 are separated by 13 nucleotides
SMa1211: SMa1211 - FixG iron sulfur membrane protein, at 665,557 to 667,131
SMa1211
SMa1213: SMa1213 - FixP1 di-heme cytochrome c, at 667,360 to 668,229
SMa1213
SMa1214: SMa1214 - FixQ1 nitrogen fixation protein, at 668,233 to 668,385
SMa1214
SMa1216: SMa1216 - cbb3-type cytochrome c oxidase subunit II, at 668,398 to 669,129
SMa1216
SMa1220: SMa1220 - cbb3-type cytochrome c oxidase subunit I, at 669,143 to 670,762
SMa1220
Position (kb) 667
668
669 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 666.369 kb on - strand, within SMa1211 at 666.394 kb on + strand at 666.394 kb on + strand, within SMa1211 at 666.395 kb on - strand, within SMa1211 at 666.429 kb on + strand, within SMa1211 at 666.429 kb on + strand, within SMa1211 at 666.429 kb on + strand, within SMa1211 at 666.429 kb on + strand, within SMa1211 at 666.429 kb on + strand, within SMa1211 at 666.430 kb on - strand, within SMa1211 at 666.430 kb on - strand, within SMa1211 at 666.430 kb on - strand, within SMa1211 at 666.430 kb on - strand, within SMa1211 at 666.430 kb on - strand, within SMa1211 at 666.430 kb on - strand, within SMa1211 at 666.431 kb on + strand, within SMa1211 at 666.431 kb on + strand, within SMa1211 at 666.431 kb on + strand, within SMa1211 at 666.431 kb on + strand, within SMa1211 at 666.431 kb on + strand, within SMa1211 at 666.432 kb on - strand, within SMa1211 at 666.432 kb on - strand, within SMa1211 at 666.432 kb on - strand, within SMa1211 at 666.432 kb on - strand, within SMa1211 at 666.432 kb on - strand, within SMa1211 at 666.432 kb on - strand, within SMa1211 at 666.470 kb on + strand, within SMa1211 at 666.471 kb on - strand, within SMa1211 at 666.533 kb on - strand, within SMa1211 at 666.566 kb on + strand, within SMa1211 at 666.567 kb on - strand, within SMa1211 at 666.567 kb on - strand at 666.567 kb on - strand, within SMa1211 at 666.637 kb on + strand, within SMa1211 at 666.870 kb on - strand, within SMa1211 at 666.953 kb on + strand, within SMa1211 at 666.953 kb on + strand, within SMa1211 at 666.956 kb on + strand, within SMa1211 at 667.151 kb on - strand at 667.361 kb on + strand at 667.653 kb on - strand, within SMa1213 at 667.653 kb on - strand, within SMa1213 at 667.758 kb on + strand, within SMa1213 at 667.767 kb on - strand, within SMa1213 at 667.767 kb on - strand, within SMa1213 at 667.767 kb on - strand, within SMa1213 at 668.085 kb on - strand, within SMa1213 at 668.112 kb on - strand at 668.120 kb on + strand, within SMa1213 at 668.146 kb on - strand at 668.375 kb on + strand at 668.376 kb on - strand at 668.376 kb on - strand at 668.423 kb on + strand at 668.621 kb on + strand, within SMa1216 at 668.621 kb on + strand, within SMa1216 at 668.702 kb on + strand, within SMa1216 at 668.703 kb on - strand, within SMa1216 at 668.703 kb on - strand, within SMa1216 at 668.774 kb on + strand, within SMa1216 at 668.840 kb on + strand, within SMa1216 at 668.840 kb on + strand, within SMa1216 at 668.840 kb on + strand, within SMa1216 at 668.840 kb on + strand, within SMa1216 at 668.841 kb on - strand, within SMa1216 at 668.865 kb on - strand, within SMa1216 at 668.924 kb on + strand, within SMa1216 at 668.925 kb on - strand, within SMa1216 at 668.925 kb on - strand, within SMa1216 at 668.925 kb on - strand, within SMa1216 at 668.942 kb on + strand, within SMa1216 at 668.942 kb on + strand, within SMa1216 at 668.943 kb on - strand, within SMa1216 at 668.943 kb on - strand, within SMa1216 at 668.975 kb on + strand, within SMa1216 at 668.975 kb on + strand, within SMa1216 at 668.975 kb on + strand, within SMa1216 at 668.976 kb on - strand, within SMa1216 at 669.017 kb on + strand, within SMa1216 at 669.073 kb on + strand at 669.074 kb on - strand at 669.135 kb on - strand at 669.135 kb on - strand at 669.135 kb on - strand at 669.144 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 666,369 - SMa1211 0.52 -0.2 666,394 + +0.5 666,394 + SMa1211 0.53 +2.3 666,395 - SMa1211 0.53 +0.5 666,429 + SMa1211 0.55 -1.2 666,429 + SMa1211 0.55 -1.0 666,429 + SMa1211 0.55 -1.2 666,429 + SMa1211 0.55 -0.1 666,429 + SMa1211 0.55 +0.3 666,430 - SMa1211 0.55 -0.2 666,430 - SMa1211 0.55 +1.5 666,430 - SMa1211 0.55 +0.9 666,430 - SMa1211 0.55 -0.5 666,430 - SMa1211 0.55 +0.7 666,430 - SMa1211 0.55 -2.2 666,431 + SMa1211 0.55 -0.4 666,431 + SMa1211 0.55 +0.4 666,431 + SMa1211 0.55 -0.4 666,431 + SMa1211 0.55 +0.5 666,431 + SMa1211 0.55 -0.1 666,432 - SMa1211 0.56 -0.2 666,432 - SMa1211 0.56 -0.6 666,432 - SMa1211 0.56 -0.5 666,432 - SMa1211 0.56 +1.0 666,432 - SMa1211 0.56 -0.8 666,432 - SMa1211 0.56 -1.1 666,470 + SMa1211 0.58 +1.1 666,471 - SMa1211 0.58 +0.6 666,533 - SMa1211 0.62 -0.2 666,566 + SMa1211 0.64 -1.1 666,567 - SMa1211 0.64 +0.2 666,567 - -0.1 666,567 - SMa1211 0.64 -0.3 666,637 + SMa1211 0.69 -0.1 666,870 - SMa1211 0.83 -2.2 666,953 + SMa1211 0.89 +0.1 666,953 + SMa1211 0.89 -0.5 666,956 + SMa1211 0.89 -0.1 667,151 - +0.2 667,361 + -0.3 667,653 - SMa1213 0.34 -1.6 667,653 - SMa1213 0.34 +0.5 667,758 + SMa1213 0.46 +1.5 667,767 - SMa1213 0.47 -0.2 667,767 - SMa1213 0.47 -0.8 667,767 - SMa1213 0.47 -1.0 668,085 - SMa1213 0.83 -0.1 668,112 - -0.1 668,120 + SMa1213 0.87 +0.6 668,146 - +0.2 668,375 + -1.1 668,376 - -0.7 668,376 - -0.7 668,423 + +1.0 668,621 + SMa1216 0.30 +0.3 668,621 + SMa1216 0.30 +0.5 668,702 + SMa1216 0.42 -1.9 668,703 - SMa1216 0.42 +0.9 668,703 - SMa1216 0.42 -0.0 668,774 + SMa1216 0.51 +0.4 668,840 + SMa1216 0.60 -2.1 668,840 + SMa1216 0.60 -0.8 668,840 + SMa1216 0.60 +3.5 668,840 + SMa1216 0.60 +0.0 668,841 - SMa1216 0.61 +0.5 668,865 - SMa1216 0.64 -0.4 668,924 + SMa1216 0.72 +0.9 668,925 - SMa1216 0.72 +1.0 668,925 - SMa1216 0.72 -0.1 668,925 - SMa1216 0.72 -0.2 668,942 + SMa1216 0.74 +0.5 668,942 + SMa1216 0.74 +0.1 668,943 - SMa1216 0.74 -1.1 668,943 - SMa1216 0.74 +0.4 668,975 + SMa1216 0.79 +2.4 668,975 + SMa1216 0.79 -0.9 668,975 + SMa1216 0.79 -0.6 668,976 - SMa1216 0.79 -0.7 669,017 + SMa1216 0.85 -1.4 669,073 + -0.3 669,074 - +1.5 669,135 - +2.7 669,135 - -0.2 669,135 - +0.9 669,144 + -0.6
Or see this region's nucleotide sequence