Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa5004 and SMa5029 are separated by 64 nucleotides SMa5029 and SMa0846 are separated by 20 nucleotides SMa0846 and SMa0848 are separated by 323 nucleotides SMa0848 and SMa0849 are separated by 57 nucleotides
SMa5004: SMa5004 - transposase, fragment, at 464,811 to 465,101
SMa5004
SMa5029: SMa5029 - transposase, fragment, at 465,166 to 465,342
SMa5029
SMa0846: SMa0846 - transposase, fragment, at 465,363 to 465,620
SMa0846
SMa0848: SMa0848 - hypothetical protein, at 465,944 to 466,360
SMa0848
SMa0849: SMa0849 - SyrM transcriptional regulator, at 466,418 to 467,398
SMa0849
Position (kb) 465
466
467 Strain fitness (log2 ratio) -2
-1
0
1 at 465.172 kb on + strand at 465.173 kb on - strand at 465.370 kb on + strand at 465.370 kb on + strand at 465.514 kb on + strand, within SMa0846 at 465.618 kb on + strand at 465.926 kb on + strand at 465.927 kb on - strand at 465.927 kb on - strand at 465.927 kb on - strand at 466.007 kb on + strand, within SMa0848 at 466.009 kb on + strand, within SMa0848 at 466.179 kb on - strand, within SMa0848 at 466.179 kb on - strand, within SMa0848 at 466.279 kb on - strand, within SMa0848 at 466.411 kb on + strand at 466.411 kb on + strand at 466.411 kb on + strand at 466.447 kb on + strand at 466.519 kb on - strand, within SMa0849 at 466.525 kb on - strand, within SMa0849 at 466.525 kb on - strand, within SMa0849 at 466.564 kb on + strand, within SMa0849 at 466.667 kb on - strand, within SMa0849 at 466.723 kb on - strand, within SMa0849 at 466.909 kb on - strand, within SMa0849 at 467.176 kb on - strand, within SMa0849 at 467.223 kb on + strand, within SMa0849 at 467.303 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 465,172 + -1.4 465,173 - -0.2 465,370 + -1.7 465,370 + -0.5 465,514 + SMa0846 0.59 -1.1 465,618 + -2.0 465,926 + -2.6 465,927 - +0.5 465,927 - +1.5 465,927 - -0.5 466,007 + SMa0848 0.15 -2.0 466,009 + SMa0848 0.16 -2.4 466,179 - SMa0848 0.56 -1.4 466,179 - SMa0848 0.56 -2.7 466,279 - SMa0848 0.80 -0.4 466,411 + -0.2 466,411 + -1.1 466,411 + +1.2 466,447 + -1.4 466,519 - SMa0849 0.10 +0.4 466,525 - SMa0849 0.11 -0.8 466,525 - SMa0849 0.11 +0.8 466,564 + SMa0849 0.15 +0.0 466,667 - SMa0849 0.25 -0.4 466,723 - SMa0849 0.31 +1.5 466,909 - SMa0849 0.50 +1.0 467,176 - SMa0849 0.77 +0.3 467,223 + SMa0849 0.82 -0.6 467,303 + -0.1
Or see this region's nucleotide sequence