Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0840 and SMa0841 are separated by 660 nucleotides SMa0841 and SMa5004 are separated by 8 nucleotides SMa5004 and SMa5029 are separated by 64 nucleotides SMa5029 and SMa0846 are separated by 20 nucleotides
SMa0840: SMa0840 - NodD3 nod box-dependent transcriptional activator, at 462,751 to 463,692
SMa0840
SMa0841: SMa0841 - transposase, fragment, at 464,353 to 464,802
SMa0841
SMa5004: SMa5004 - transposase, fragment, at 464,811 to 465,101
SMa5004
SMa5029: SMa5029 - transposase, fragment, at 465,166 to 465,342
SMa5029
SMa0846: SMa0846 - transposase, fragment, at 465,363 to 465,620
SMa0846
Position (kb) 464
465 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 463.363 kb on + strand, within SMa0840 at 463.453 kb on + strand, within SMa0840 at 463.453 kb on + strand, within SMa0840 at 463.453 kb on + strand, within SMa0840 at 463.454 kb on - strand, within SMa0840 at 463.454 kb on - strand, within SMa0840 at 463.512 kb on + strand, within SMa0840 at 463.512 kb on + strand, within SMa0840 at 463.705 kb on - strand at 463.863 kb on + strand at 463.976 kb on + strand at 463.980 kb on + strand at 464.043 kb on + strand at 464.152 kb on + strand at 464.348 kb on - strand at 464.363 kb on + strand at 464.421 kb on + strand, within SMa0841 at 464.525 kb on + strand, within SMa0841 at 464.540 kb on + strand, within SMa0841 at 464.621 kb on - strand, within SMa0841 at 464.621 kb on - strand, within SMa0841 at 465.172 kb on + strand at 465.173 kb on - strand at 465.370 kb on + strand at 465.370 kb on + strand at 465.514 kb on + strand, within SMa0846 at 465.618 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 463,363 + SMa0840 0.65 -0.6 463,453 + SMa0840 0.75 -0.4 463,453 + SMa0840 0.75 +0.8 463,453 + SMa0840 0.75 +2.0 463,454 - SMa0840 0.75 -1.5 463,454 - SMa0840 0.75 +0.9 463,512 + SMa0840 0.81 +0.3 463,512 + SMa0840 0.81 -0.3 463,705 - -3.3 463,863 + +0.5 463,976 + +1.4 463,980 + -0.6 464,043 + +0.8 464,152 + +1.1 464,348 - +0.2 464,363 + +2.0 464,421 + SMa0841 0.15 -0.7 464,525 + SMa0841 0.38 -1.7 464,540 + SMa0841 0.42 -0.9 464,621 - SMa0841 0.60 -1.7 464,621 - SMa0841 0.60 -1.6 465,172 + -1.4 465,173 - -0.2 465,370 + -1.7 465,370 + -0.5 465,514 + SMa0846 0.59 -1.1 465,618 + -2.0
Or see this region's nucleotide sequence