Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20060 and SM_b22003 are separated by 158 nucleotides SM_b22003 and SM_b20061 are separated by 160 nucleotides SM_b20061 and SM_b20062 are separated by 103 nucleotides SM_b20062 and SM_b20063 overlap by 11 nucleotides SM_b20063 and SM_b20064 overlap by 79 nucleotides
SM_b20060: SM_b20060 - ISRm5 transposase, at 70,423 to 71,619
_b20060
SM_b22003: SM_b22003 - hypothetical protein, at 71,778 to 72,098
_b22003
SM_b20061: SM_b20061 - hypothetical protein, at 72,259 to 72,414
_b20061
SM_b20062: SM_b20062 - hypothetical protein, at 72,518 to 72,766
_b20062
SM_b20063: SM_b20063 - hypothetical protein, at 72,756 to 73,052
_b20063
SM_b20064: SM_b20064 - hypothetical protein, at 72,974 to 73,420
_b20064
Position (kb)
71
72
73 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 71.693 kb on - strand at 71.755 kb on + strand at 71.756 kb on - strand at 71.756 kb on - strand at 71.792 kb on + strand at 71.792 kb on + strand at 71.811 kb on + strand, within SM_b22003 at 71.811 kb on + strand, within SM_b22003 at 71.811 kb on + strand, within SM_b22003 at 71.812 kb on - strand, within SM_b22003 at 71.881 kb on + strand, within SM_b22003 at 71.882 kb on - strand, within SM_b22003 at 71.882 kb on - strand, within SM_b22003 at 71.882 kb on - strand, within SM_b22003 at 71.882 kb on - strand, within SM_b22003 at 71.950 kb on + strand, within SM_b22003 at 71.988 kb on + strand, within SM_b22003 at 71.989 kb on - strand, within SM_b22003 at 72.103 kb on - strand at 72.103 kb on - strand at 72.108 kb on + strand at 72.151 kb on + strand at 72.152 kb on - strand at 72.218 kb on + strand at 72.218 kb on + strand at 72.218 kb on + strand at 72.218 kb on + strand at 72.218 kb on + strand at 72.219 kb on - strand at 72.271 kb on + strand at 72.271 kb on + strand at 72.432 kb on + strand at 72.432 kb on + strand at 72.432 kb on + strand at 72.432 kb on + strand at 72.432 kb on + strand at 72.432 kb on + strand at 72.444 kb on - strand at 72.512 kb on - strand at 72.512 kb on - strand at 72.512 kb on - strand at 72.512 kb on - strand at 72.539 kb on - strand at 72.539 kb on - strand at 72.660 kb on + strand, within SM_b20062 at 72.823 kb on - strand, within SM_b20063 at 72.877 kb on - strand, within SM_b20063 at 72.877 kb on - strand, within SM_b20063 at 72.877 kb on - strand, within SM_b20063 at 72.877 kb on - strand, within SM_b20063 at 72.878 kb on + strand, within SM_b20063 at 72.879 kb on - strand, within SM_b20063 at 72.952 kb on - strand, within SM_b20063 at 73.094 kb on - strand, within SM_b20064
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 71,693 - -0.7 71,755 + -0.4 71,756 - -0.2 71,756 - -0.1 71,792 + -0.5 71,792 + -0.4 71,811 + SM_b22003 0.10 -0.9 71,811 + SM_b22003 0.10 -0.9 71,811 + SM_b22003 0.10 -1.3 71,812 - SM_b22003 0.11 +0.4 71,881 + SM_b22003 0.32 +0.4 71,882 - SM_b22003 0.32 +0.4 71,882 - SM_b22003 0.32 -0.5 71,882 - SM_b22003 0.32 -0.8 71,882 - SM_b22003 0.32 -1.1 71,950 + SM_b22003 0.54 -1.3 71,988 + SM_b22003 0.65 +0.4 71,989 - SM_b22003 0.66 -2.3 72,103 - +0.5 72,103 - -2.7 72,108 + +1.2 72,151 + -1.0 72,152 - +0.6 72,218 + +1.0 72,218 + -0.4 72,218 + +0.8 72,218 + -0.4 72,218 + -1.1 72,219 - +0.1 72,271 + -3.1 72,271 + -0.4 72,432 + -0.4 72,432 + -2.1 72,432 + -0.2 72,432 + -1.7 72,432 + -0.0 72,432 + -1.5 72,444 - +0.0 72,512 - -0.7 72,512 - -0.4 72,512 - +0.4 72,512 - -2.0 72,539 - +0.9 72,539 - +1.5 72,660 + SM_b20062 0.57 -2.8 72,823 - SM_b20063 0.23 +1.8 72,877 - SM_b20063 0.41 -0.1 72,877 - SM_b20063 0.41 +0.2 72,877 - SM_b20063 0.41 +0.4 72,877 - SM_b20063 0.41 -2.0 72,878 + SM_b20063 0.41 +0.2 72,879 - SM_b20063 0.41 -0.2 72,952 - SM_b20063 0.66 -0.6 73,094 - SM_b20064 0.27 -0.7
Or see this region's nucleotide sequence