Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b22004 and SM_b20121 overlap by 56 nucleotides SM_b20121 and SM_b20122 are separated by 181 nucleotides SM_b20122 and SM_b20123 are separated by 185 nucleotides SM_b20123 and SM_b20124 are separated by 121 nucleotides
SM_b22004: SM_b22004 - plasmid stabilization system protein, at 132,946 to 133,338
_b22004
SM_b20121: SM_b20121 - hypothetical protein, at 133,283 to 133,558
_b20121
SM_b20122: SM_b20122 - hypothetical protein, at 133,740 to 134,075
_b20122
SM_b20123: SM_b20123 - transcriptional regulator, at 134,261 to 135,157
_b20123
SM_b20124: SM_b20124 - sugar ABC transporter ATP-binding protein, at 135,279 to 136,805
_b20124
Position (kb)
134
135
136 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 133.368 kb on - strand, within SM_b20121 at 133.476 kb on + strand, within SM_b20121 at 133.609 kb on - strand at 133.610 kb on + strand at 133.654 kb on + strand at 133.654 kb on + strand at 133.654 kb on + strand at 133.689 kb on + strand at 133.689 kb on + strand at 133.702 kb on + strand at 133.723 kb on - strand at 133.723 kb on - strand at 133.830 kb on - strand, within SM_b20122 at 133.885 kb on + strand, within SM_b20122 at 133.885 kb on + strand, within SM_b20122 at 133.885 kb on + strand, within SM_b20122 at 133.886 kb on - strand, within SM_b20122 at 133.930 kb on - strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.035 kb on - strand, within SM_b20122 at 134.035 kb on - strand, within SM_b20122 at 134.035 kb on - strand, within SM_b20122 at 134.092 kb on + strand at 134.097 kb on + strand at 134.496 kb on + strand, within SM_b20123 at 134.497 kb on - strand, within SM_b20123 at 134.793 kb on - strand, within SM_b20123 at 134.793 kb on - strand, within SM_b20123 at 134.922 kb on - strand, within SM_b20123 at 135.070 kb on + strand at 135.098 kb on + strand at 135.162 kb on - strand at 135.253 kb on - strand at 135.376 kb on + strand at 135.376 kb on + strand at 135.376 kb on + strand at 135.377 kb on - strand at 135.377 kb on - strand at 135.377 kb on - strand at 135.516 kb on - strand, within SM_b20124 at 135.792 kb on - strand, within SM_b20124 at 135.821 kb on - strand, within SM_b20124 at 135.972 kb on + strand, within SM_b20124 at 135.972 kb on + strand, within SM_b20124 at 135.973 kb on - strand at 135.973 kb on - strand, within SM_b20124 at 136.069 kb on + strand, within SM_b20124 at 136.070 kb on - strand, within SM_b20124 at 136.140 kb on - strand, within SM_b20124
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 133,368 - SM_b20121 0.31 -0.2 133,476 + SM_b20121 0.70 +0.6 133,609 - -1.7 133,610 + -2.2 133,654 + -2.7 133,654 + -0.1 133,654 + +0.1 133,689 + -2.0 133,689 + +0.1 133,702 + +0.1 133,723 - -0.7 133,723 - -2.8 133,830 - SM_b20122 0.27 -1.3 133,885 + SM_b20122 0.43 -0.8 133,885 + SM_b20122 0.43 +0.1 133,885 + SM_b20122 0.43 -0.3 133,886 - SM_b20122 0.43 +0.2 133,930 - SM_b20122 0.57 -2.0 134,034 + SM_b20122 0.88 -0.7 134,034 + SM_b20122 0.88 -0.2 134,034 + SM_b20122 0.88 -0.0 134,034 + SM_b20122 0.88 -0.3 134,034 + SM_b20122 0.88 -1.8 134,035 - SM_b20122 0.88 +0.1 134,035 - SM_b20122 0.88 -0.5 134,035 - SM_b20122 0.88 +0.5 134,092 + -2.8 134,097 + -0.3 134,496 + SM_b20123 0.26 -1.2 134,497 - SM_b20123 0.26 -1.4 134,793 - SM_b20123 0.59 -2.3 134,793 - SM_b20123 0.59 -0.5 134,922 - SM_b20123 0.74 +0.0 135,070 + -1.1 135,098 + -1.0 135,162 - -0.3 135,253 - +1.1 135,376 + +0.3 135,376 + -0.4 135,376 + +0.2 135,377 - -3.1 135,377 - -1.4 135,377 - -1.5 135,516 - SM_b20124 0.16 +0.7 135,792 - SM_b20124 0.34 -1.4 135,821 - SM_b20124 0.35 -0.1 135,972 + SM_b20124 0.45 +0.4 135,972 + SM_b20124 0.45 +0.4 135,973 - +0.6 135,973 - SM_b20124 0.45 +0.2 136,069 + SM_b20124 0.52 -0.1 136,070 - SM_b20124 0.52 -1.5 136,140 - SM_b20124 0.56 -0.1
Or see this region's nucleotide sequence