Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_555

Experiment: Cobalt chloride 0.6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_554 and Pf6N2E2_555 are separated by 29 nucleotidesPf6N2E2_555 and Pf6N2E2_556 are separated by 83 nucleotidesPf6N2E2_556 and Pf6N2E2_557 are separated by 24 nucleotidesPf6N2E2_557 and Pf6N2E2_558 are separated by 63 nucleotides Pf6N2E2_554 - Uncharacterized protein ImpC, at 625,804 to 627,285 _554 Pf6N2E2_555 - Uncharacterized protein ImpB, at 627,315 to 627,875 _555 Pf6N2E2_556 - hypothetical protein, at 627,959 to 628,075 _556 Pf6N2E2_557 - Uncharacterized protein homologous to Paeru. PA2364, at 628,100 to 628,549 _557 Pf6N2E2_558 - Uncharacterized protein ImpJ/VasE, at 628,613 to 629,944 _558 Position (kb) 627 628Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 626.344 kb on - strand, within Pf6N2E2_554at 626.344 kb on - strand, within Pf6N2E2_554at 626.408 kb on + strand, within Pf6N2E2_554at 626.408 kb on + strand, within Pf6N2E2_554at 626.408 kb on + strand, within Pf6N2E2_554at 626.409 kb on - strand, within Pf6N2E2_554at 626.409 kb on - strand, within Pf6N2E2_554at 626.409 kb on - strand, within Pf6N2E2_554at 626.409 kb on - strand, within Pf6N2E2_554at 626.409 kb on - strand, within Pf6N2E2_554at 626.409 kb on - strand, within Pf6N2E2_554at 626.468 kb on + strand, within Pf6N2E2_554at 626.469 kb on - strand, within Pf6N2E2_554at 626.469 kb on - strand, within Pf6N2E2_554at 626.469 kb on - strand, within Pf6N2E2_554at 626.469 kb on - strand, within Pf6N2E2_554at 626.469 kb on - strand, within Pf6N2E2_554at 626.504 kb on + strand, within Pf6N2E2_554at 626.504 kb on + strand, within Pf6N2E2_554at 626.505 kb on - strand, within Pf6N2E2_554at 626.505 kb on - strand, within Pf6N2E2_554at 626.505 kb on - strand, within Pf6N2E2_554at 626.505 kb on - strand, within Pf6N2E2_554at 626.582 kb on + strand, within Pf6N2E2_554at 626.798 kb on + strand, within Pf6N2E2_554at 626.799 kb on - strand, within Pf6N2E2_554at 626.806 kb on + strand, within Pf6N2E2_554at 626.843 kb on + strand, within Pf6N2E2_554at 626.844 kb on - strand, within Pf6N2E2_554at 626.844 kb on - strand, within Pf6N2E2_554at 626.855 kb on + strand, within Pf6N2E2_554at 626.855 kb on + strand, within Pf6N2E2_554at 626.855 kb on + strand, within Pf6N2E2_554at 626.856 kb on - strand, within Pf6N2E2_554at 627.173 kb on + strandat 627.291 kb on + strandat 627.291 kb on + strandat 627.379 kb on - strand, within Pf6N2E2_555at 627.814 kb on + strand, within Pf6N2E2_555at 627.814 kb on + strandat 627.814 kb on + strand, within Pf6N2E2_555at 627.814 kb on + strand, within Pf6N2E2_555at 627.815 kb on - strand, within Pf6N2E2_555at 627.815 kb on - strand, within Pf6N2E2_555at 627.864 kb on - strandat 628.023 kb on + strand, within Pf6N2E2_556at 628.036 kb on - strand, within Pf6N2E2_556at 628.445 kb on + strand, within Pf6N2E2_557at 628.445 kb on + strand, within Pf6N2E2_557at 628.445 kb on + strand, within Pf6N2E2_557at 628.445 kb on + strand, within Pf6N2E2_557at 628.445 kb on + strand, within Pf6N2E2_557at 628.445 kb on + strand, within Pf6N2E2_557at 628.445 kb on + strand, within Pf6N2E2_557at 628.445 kb on + strand, within Pf6N2E2_557at 628.446 kb on - strand, within Pf6N2E2_557at 628.446 kb on - strand, within Pf6N2E2_557at 628.446 kb on - strand, within Pf6N2E2_557at 628.446 kb on - strand, within Pf6N2E2_557at 628.448 kb on - strand, within Pf6N2E2_557at 628.448 kb on - strand, within Pf6N2E2_557at 628.448 kb on - strand, within Pf6N2E2_557at 628.612 kb on + strandat 628.613 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM
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626,344 - Pf6N2E2_554 0.36 -0.9
626,344 - Pf6N2E2_554 0.36 -0.4
626,408 + Pf6N2E2_554 0.41 -1.3
626,408 + Pf6N2E2_554 0.41 -0.9
626,408 + Pf6N2E2_554 0.41 -1.9
626,409 - Pf6N2E2_554 0.41 -0.7
626,409 - Pf6N2E2_554 0.41 -1.7
626,409 - Pf6N2E2_554 0.41 -0.3
626,409 - Pf6N2E2_554 0.41 -0.3
626,409 - Pf6N2E2_554 0.41 -1.6
626,409 - Pf6N2E2_554 0.41 +0.7
626,468 + Pf6N2E2_554 0.45 +0.7
626,469 - Pf6N2E2_554 0.45 +0.4
626,469 - Pf6N2E2_554 0.45 +1.7
626,469 - Pf6N2E2_554 0.45 +0.9
626,469 - Pf6N2E2_554 0.45 -2.4
626,469 - Pf6N2E2_554 0.45 -1.7
626,504 + Pf6N2E2_554 0.47 -0.0
626,504 + Pf6N2E2_554 0.47 +0.4
626,505 - Pf6N2E2_554 0.47 +0.0
626,505 - Pf6N2E2_554 0.47 +1.7
626,505 - Pf6N2E2_554 0.47 +0.1
626,505 - Pf6N2E2_554 0.47 -0.9
626,582 + Pf6N2E2_554 0.52 +0.4
626,798 + Pf6N2E2_554 0.67 -0.4
626,799 - Pf6N2E2_554 0.67 +2.0
626,806 + Pf6N2E2_554 0.68 -0.9
626,843 + Pf6N2E2_554 0.70 -1.9
626,844 - Pf6N2E2_554 0.70 -0.3
626,844 - Pf6N2E2_554 0.70 +1.0
626,855 + Pf6N2E2_554 0.71 -0.1
626,855 + Pf6N2E2_554 0.71 -0.7
626,855 + Pf6N2E2_554 0.71 -2.9
626,856 - Pf6N2E2_554 0.71 -0.5
627,173 + +0.1
627,291 + -1.0
627,291 + -3.1
627,379 - Pf6N2E2_555 0.11 -0.5
627,814 + Pf6N2E2_555 0.89 +0.7
627,814 + +0.7
627,814 + Pf6N2E2_555 0.89 -1.3
627,814 + Pf6N2E2_555 0.89 -0.8
627,815 - Pf6N2E2_555 0.89 -2.4
627,815 - Pf6N2E2_555 0.89 -0.5
627,864 - +0.7
628,023 + Pf6N2E2_556 0.55 -1.9
628,036 - Pf6N2E2_556 0.66 -0.1
628,445 + Pf6N2E2_557 0.77 -0.5
628,445 + Pf6N2E2_557 0.77 -0.5
628,445 + Pf6N2E2_557 0.77 -0.3
628,445 + Pf6N2E2_557 0.77 -0.3
628,445 + Pf6N2E2_557 0.77 -2.0
628,445 + Pf6N2E2_557 0.77 -1.9
628,445 + Pf6N2E2_557 0.77 -2.0
628,445 + Pf6N2E2_557 0.77 -2.3
628,446 - Pf6N2E2_557 0.77 +0.7
628,446 - Pf6N2E2_557 0.77 +0.9
628,446 - Pf6N2E2_557 0.77 +0.7
628,446 - Pf6N2E2_557 0.77 -0.0
628,448 - Pf6N2E2_557 0.77 -1.6
628,448 - Pf6N2E2_557 0.77 -0.7
628,448 - Pf6N2E2_557 0.77 +0.3
628,612 + +0.7
628,613 - +0.2

Or see this region's nucleotide sequence