Experiment: Cobalt chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_445 and Pf6N2E2_446 are separated by 200 nucleotides Pf6N2E2_446 and Pf6N2E2_447 are separated by 174 nucleotides Pf6N2E2_447 and Pf6N2E2_448 are separated by 124 nucleotides
Pf6N2E2_445 - UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), at 507,127 to 507,966
_445
Pf6N2E2_446 - Glutathione reductase (EC 1.8.1.7), at 508,167 to 509,525
_446
Pf6N2E2_447 - Alkyl hydroperoxide reductase protein C (EC 1.6.4.-), at 509,700 to 510,263
_447
Pf6N2E2_448 - Alkyl hydroperoxide reductase protein F (EC 1.6.4.-), at 510,388 to 511,950
_448
Position (kb)
508
509
510 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 507.186 kb on + strand at 507.187 kb on - strand at 507.187 kb on - strand at 507.334 kb on + strand, within Pf6N2E2_445 at 507.334 kb on + strand, within Pf6N2E2_445 at 507.335 kb on - strand, within Pf6N2E2_445 at 507.376 kb on + strand, within Pf6N2E2_445 at 507.421 kb on + strand, within Pf6N2E2_445 at 507.548 kb on + strand, within Pf6N2E2_445 at 507.548 kb on + strand, within Pf6N2E2_445 at 507.571 kb on + strand, within Pf6N2E2_445 at 507.571 kb on + strand, within Pf6N2E2_445 at 507.635 kb on - strand, within Pf6N2E2_445 at 507.673 kb on + strand, within Pf6N2E2_445 at 507.673 kb on + strand, within Pf6N2E2_445 at 507.746 kb on - strand, within Pf6N2E2_445 at 507.862 kb on + strand, within Pf6N2E2_445 at 507.862 kb on + strand, within Pf6N2E2_445 at 507.863 kb on - strand, within Pf6N2E2_445 at 507.863 kb on - strand, within Pf6N2E2_445 at 507.907 kb on + strand at 507.908 kb on - strand at 507.908 kb on - strand at 507.943 kb on + strand at 507.943 kb on + strand at 507.943 kb on + strand at 507.943 kb on + strand at 507.944 kb on - strand at 507.944 kb on - strand at 507.944 kb on - strand at 507.961 kb on + strand at 508.137 kb on + strand at 508.138 kb on - strand at 508.138 kb on - strand at 508.138 kb on - strand at 508.138 kb on - strand at 508.138 kb on - strand at 508.162 kb on + strand at 508.162 kb on + strand at 508.162 kb on + strand at 508.163 kb on - strand at 508.163 kb on - strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.188 kb on + strand at 508.189 kb on - strand at 508.189 kb on - strand at 508.189 kb on - strand at 508.189 kb on - strand at 508.189 kb on - strand at 508.217 kb on - strand at 508.329 kb on + strand, within Pf6N2E2_446 at 508.330 kb on - strand, within Pf6N2E2_446 at 508.341 kb on + strand at 508.341 kb on + strand, within Pf6N2E2_446 at 508.371 kb on + strand, within Pf6N2E2_446 at 508.372 kb on - strand, within Pf6N2E2_446 at 508.458 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.750 kb on + strand, within Pf6N2E2_446 at 508.751 kb on - strand, within Pf6N2E2_446 at 508.751 kb on - strand, within Pf6N2E2_446 at 508.751 kb on - strand, within Pf6N2E2_446 at 508.751 kb on - strand, within Pf6N2E2_446 at 508.751 kb on - strand, within Pf6N2E2_446 at 508.751 kb on - strand, within Pf6N2E2_446 at 508.751 kb on - strand, within Pf6N2E2_446 at 508.938 kb on + strand, within Pf6N2E2_446 at 508.938 kb on + strand, within Pf6N2E2_446 at 508.938 kb on + strand, within Pf6N2E2_446 at 508.939 kb on - strand, within Pf6N2E2_446 at 509.034 kb on + strand, within Pf6N2E2_446 at 509.375 kb on + strand, within Pf6N2E2_446 at 509.375 kb on + strand, within Pf6N2E2_446 at 509.507 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.541 kb on - strand at 509.541 kb on - strand at 509.541 kb on - strand at 509.541 kb on - strand at 509.580 kb on - strand at 509.583 kb on + strand at 509.584 kb on - strand at 509.656 kb on + strand at 509.656 kb on + strand at 509.656 kb on + strand at 509.706 kb on - strand at 509.706 kb on - strand at 509.706 kb on - strand at 509.724 kb on - strand at 509.814 kb on + strand, within Pf6N2E2_447 at 509.814 kb on + strand, within Pf6N2E2_447 at 509.904 kb on + strand, within Pf6N2E2_447 at 509.905 kb on - strand, within Pf6N2E2_447 at 509.905 kb on - strand, within Pf6N2E2_447 at 509.979 kb on + strand, within Pf6N2E2_447 at 509.980 kb on - strand, within Pf6N2E2_447 at 509.980 kb on - strand, within Pf6N2E2_447 at 509.980 kb on - strand, within Pf6N2E2_447 at 510.169 kb on - strand, within Pf6N2E2_447 at 510.169 kb on - strand, within Pf6N2E2_447 at 510.169 kb on - strand, within Pf6N2E2_447 at 510.261 kb on + strand at 510.261 kb on + strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.298 kb on + strand at 510.298 kb on + strand at 510.298 kb on + strand at 510.298 kb on + strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.387 kb on + strand at 510.388 kb on - strand at 510.388 kb on - strand at 510.405 kb on + strand at 510.405 kb on + strand at 510.424 kb on + strand at 510.504 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM remove 507,186 + -1.2 507,187 - -0.5 507,187 - -2.2 507,334 + Pf6N2E2_445 0.25 -0.4 507,334 + Pf6N2E2_445 0.25 -0.4 507,335 - Pf6N2E2_445 0.25 -0.2 507,376 + Pf6N2E2_445 0.30 -1.2 507,421 + Pf6N2E2_445 0.35 +1.4 507,548 + Pf6N2E2_445 0.50 -2.2 507,548 + Pf6N2E2_445 0.50 -1.2 507,571 + Pf6N2E2_445 0.53 -0.0 507,571 + Pf6N2E2_445 0.53 -1.3 507,635 - Pf6N2E2_445 0.60 -0.3 507,673 + Pf6N2E2_445 0.65 -1.0 507,673 + Pf6N2E2_445 0.65 +0.3 507,746 - Pf6N2E2_445 0.74 -2.5 507,862 + Pf6N2E2_445 0.88 -1.2 507,862 + Pf6N2E2_445 0.88 +0.8 507,863 - Pf6N2E2_445 0.88 +0.1 507,863 - Pf6N2E2_445 0.88 +0.6 507,907 + -0.6 507,908 - -0.6 507,908 - -1.8 507,943 + +0.6 507,943 + -0.8 507,943 + -0.2 507,943 + -1.6 507,944 - +2.0 507,944 - +2.4 507,944 - -1.2 507,961 + +1.0 508,137 + -1.5 508,138 - +0.4 508,138 - +0.5 508,138 - -0.0 508,138 - +0.4 508,138 - +1.4 508,162 + +0.6 508,162 + +1.5 508,162 + +1.1 508,163 - -2.7 508,163 - -1.2 508,188 + -1.8 508,188 + -2.3 508,188 + -1.8 508,188 + -0.8 508,188 + -1.2 508,188 + -0.8 508,188 + -3.0 508,188 + -0.2 508,188 + +0.8 508,188 + -2.4 508,188 + +0.8 508,188 + -0.2 508,189 - -1.8 508,189 - -1.2 508,189 - -2.2 508,189 - -1.9 508,189 - -1.5 508,217 - -0.8 508,329 + Pf6N2E2_446 0.12 -0.8 508,330 - Pf6N2E2_446 0.12 -1.5 508,341 + +0.8 508,341 + Pf6N2E2_446 0.13 -1.8 508,371 + Pf6N2E2_446 0.15 -1.2 508,372 - Pf6N2E2_446 0.15 -1.8 508,458 + Pf6N2E2_446 0.21 -2.7 508,750 + Pf6N2E2_446 0.43 -1.2 508,750 + Pf6N2E2_446 0.43 -2.2 508,750 + Pf6N2E2_446 0.43 -1.8 508,750 + Pf6N2E2_446 0.43 -2.5 508,750 + Pf6N2E2_446 0.43 -0.2 508,750 + Pf6N2E2_446 0.43 -2.1 508,750 + Pf6N2E2_446 0.43 +0.8 508,750 + Pf6N2E2_446 0.43 -0.2 508,750 + Pf6N2E2_446 0.43 -0.8 508,750 + Pf6N2E2_446 0.43 -2.2 508,750 + Pf6N2E2_446 0.43 -1.5 508,751 - Pf6N2E2_446 0.43 +0.8 508,751 - Pf6N2E2_446 0.43 -0.8 508,751 - Pf6N2E2_446 0.43 -1.2 508,751 - Pf6N2E2_446 0.43 -2.2 508,751 - Pf6N2E2_446 0.43 -0.2 508,751 - Pf6N2E2_446 0.43 -0.2 508,751 - Pf6N2E2_446 0.43 -2.7 508,938 + Pf6N2E2_446 0.57 -1.8 508,938 + Pf6N2E2_446 0.57 -2.0 508,938 + Pf6N2E2_446 0.57 -0.2 508,939 - Pf6N2E2_446 0.57 -0.8 509,034 + Pf6N2E2_446 0.64 -1.2 509,375 + Pf6N2E2_446 0.89 -2.4 509,375 + Pf6N2E2_446 0.89 -2.8 509,507 + -1.3 509,540 + +0.0 509,540 + -1.2 509,540 + -0.2 509,540 + +1.0 509,540 + +0.8 509,540 + -0.1 509,540 + -0.2 509,540 + -0.2 509,541 - -1.2 509,541 - -1.5 509,541 - +0.2 509,541 - -1.2 509,580 - -1.0 509,583 + +0.2 509,584 - +1.4 509,656 + -0.0 509,656 + -1.2 509,656 + -0.2 509,706 - -0.2 509,706 - +2.4 509,706 - +0.0 509,724 - -0.5 509,814 + Pf6N2E2_447 0.20 -0.2 509,814 + Pf6N2E2_447 0.20 +1.8 509,904 + Pf6N2E2_447 0.36 -1.4 509,905 - Pf6N2E2_447 0.36 -0.7 509,905 - Pf6N2E2_447 0.36 +0.0 509,979 + Pf6N2E2_447 0.49 +0.8 509,980 - Pf6N2E2_447 0.50 -0.8 509,980 - Pf6N2E2_447 0.50 -1.0 509,980 - Pf6N2E2_447 0.50 +0.7 510,169 - Pf6N2E2_447 0.83 +1.2 510,169 - Pf6N2E2_447 0.83 +0.6 510,169 - Pf6N2E2_447 0.83 +1.2 510,261 + -1.1 510,261 + -1.1 510,262 - +0.4 510,262 - +1.4 510,262 - +1.3 510,262 - -0.4 510,262 - +0.1 510,262 - -1.5 510,262 - +1.0 510,298 + -1.1 510,298 + -1.5 510,298 + -1.0 510,298 + -1.7 510,299 - -0.7 510,299 - -0.2 510,299 - +0.0 510,299 - -0.2 510,299 - +1.1 510,299 - +0.3 510,299 - +0.2 510,387 + -0.2 510,388 - -0.1 510,388 - +0.1 510,405 + -0.7 510,405 + +0.8 510,424 + -1.5 510,504 - +0.8
Or see this region's nucleotide sequence