Experiment: Cobalt chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2768 and Pf6N2E2_2769 are separated by 55 nucleotides Pf6N2E2_2769 and Pf6N2E2_2770 are separated by 1 nucleotides
Pf6N2E2_2768 - Tricarboxylate transport protein TctC, at 3,256,213 to 3,257,205
_2768
Pf6N2E2_2769 - Tricarboxylate transport protein TctB, at 3,257,261 to 3,257,722
_2769
Pf6N2E2_2770 - Tricarboxylate transport membrane protein TctA, at 3,257,724 to 3,259,238
_2770
Position (kb)
3257
3258 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3256.420 kb on + strand, within Pf6N2E2_2768 at 3256.420 kb on + strand, within Pf6N2E2_2768 at 3256.420 kb on + strand, within Pf6N2E2_2768 at 3256.420 kb on + strand, within Pf6N2E2_2768 at 3256.420 kb on + strand, within Pf6N2E2_2768 at 3256.420 kb on + strand, within Pf6N2E2_2768 at 3256.421 kb on - strand, within Pf6N2E2_2768 at 3256.421 kb on - strand, within Pf6N2E2_2768 at 3256.421 kb on - strand, within Pf6N2E2_2768 at 3256.421 kb on - strand, within Pf6N2E2_2768 at 3256.421 kb on - strand, within Pf6N2E2_2768 at 3256.421 kb on - strand, within Pf6N2E2_2768 at 3256.421 kb on - strand, within Pf6N2E2_2768 at 3256.421 kb on - strand, within Pf6N2E2_2768 at 3256.421 kb on - strand, within Pf6N2E2_2768 at 3256.421 kb on - strand, within Pf6N2E2_2768 at 3256.450 kb on + strand, within Pf6N2E2_2768 at 3256.450 kb on + strand, within Pf6N2E2_2768 at 3256.450 kb on + strand, within Pf6N2E2_2768 at 3256.594 kb on + strand, within Pf6N2E2_2768 at 3256.595 kb on - strand, within Pf6N2E2_2768 at 3256.595 kb on - strand, within Pf6N2E2_2768 at 3256.649 kb on + strand, within Pf6N2E2_2768 at 3256.649 kb on + strand, within Pf6N2E2_2768 at 3256.775 kb on - strand, within Pf6N2E2_2768 at 3256.775 kb on - strand, within Pf6N2E2_2768 at 3256.775 kb on - strand, within Pf6N2E2_2768 at 3256.829 kb on + strand, within Pf6N2E2_2768 at 3256.829 kb on + strand, within Pf6N2E2_2768 at 3256.829 kb on + strand, within Pf6N2E2_2768 at 3256.829 kb on + strand, within Pf6N2E2_2768 at 3256.829 kb on + strand, within Pf6N2E2_2768 at 3256.830 kb on - strand, within Pf6N2E2_2768 at 3256.830 kb on - strand, within Pf6N2E2_2768 at 3256.830 kb on - strand at 3256.830 kb on - strand, within Pf6N2E2_2768 at 3256.830 kb on - strand, within Pf6N2E2_2768 at 3256.966 kb on + strand, within Pf6N2E2_2768 at 3256.966 kb on + strand, within Pf6N2E2_2768 at 3256.967 kb on - strand, within Pf6N2E2_2768 at 3256.999 kb on + strand, within Pf6N2E2_2768 at 3256.999 kb on + strand, within Pf6N2E2_2768 at 3257.000 kb on - strand, within Pf6N2E2_2768 at 3257.029 kb on + strand, within Pf6N2E2_2768 at 3257.029 kb on + strand, within Pf6N2E2_2768 at 3257.030 kb on - strand, within Pf6N2E2_2768 at 3257.143 kb on + strand at 3257.143 kb on + strand at 3257.143 kb on + strand at 3257.143 kb on + strand at 3257.143 kb on + strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.144 kb on - strand at 3257.167 kb on + strand at 3257.167 kb on + strand at 3257.167 kb on + strand at 3257.167 kb on + strand at 3257.168 kb on - strand at 3257.168 kb on - strand at 3257.256 kb on + strand at 3257.256 kb on + strand at 3257.256 kb on + strand at 3257.256 kb on + strand at 3257.257 kb on - strand at 3257.279 kb on - strand at 3257.354 kb on + strand, within Pf6N2E2_2769 at 3257.420 kb on + strand, within Pf6N2E2_2769 at 3257.420 kb on + strand, within Pf6N2E2_2769 at 3257.420 kb on + strand, within Pf6N2E2_2769 at 3257.420 kb on + strand, within Pf6N2E2_2769 at 3257.420 kb on + strand, within Pf6N2E2_2769 at 3257.421 kb on - strand, within Pf6N2E2_2769 at 3257.421 kb on - strand, within Pf6N2E2_2769 at 3257.453 kb on - strand, within Pf6N2E2_2769 at 3257.501 kb on - strand, within Pf6N2E2_2769 at 3257.598 kb on - strand, within Pf6N2E2_2769 at 3257.654 kb on + strand, within Pf6N2E2_2769 at 3257.654 kb on + strand, within Pf6N2E2_2769 at 3257.655 kb on - strand, within Pf6N2E2_2769 at 3257.724 kb on - strand at 3257.739 kb on + strand at 3257.739 kb on + strand at 3257.739 kb on + strand at 3257.775 kb on + strand at 3257.775 kb on + strand at 3257.775 kb on + strand at 3257.931 kb on + strand, within Pf6N2E2_2770 at 3257.931 kb on + strand, within Pf6N2E2_2770 at 3257.932 kb on - strand, within Pf6N2E2_2770 at 3257.947 kb on - strand, within Pf6N2E2_2770 at 3257.957 kb on + strand, within Pf6N2E2_2770 at 3257.957 kb on + strand, within Pf6N2E2_2770 at 3257.957 kb on + strand, within Pf6N2E2_2770 at 3257.957 kb on + strand, within Pf6N2E2_2770 at 3257.957 kb on + strand, within Pf6N2E2_2770 at 3257.958 kb on - strand, within Pf6N2E2_2770 at 3257.958 kb on - strand, within Pf6N2E2_2770 at 3257.958 kb on - strand, within Pf6N2E2_2770 at 3257.958 kb on - strand, within Pf6N2E2_2770 at 3257.958 kb on - strand, within Pf6N2E2_2770 at 3258.168 kb on + strand, within Pf6N2E2_2770 at 3258.169 kb on - strand, within Pf6N2E2_2770 at 3258.564 kb on + strand, within Pf6N2E2_2770 at 3258.564 kb on + strand, within Pf6N2E2_2770 at 3258.564 kb on + strand, within Pf6N2E2_2770 at 3258.564 kb on + strand, within Pf6N2E2_2770 at 3258.564 kb on + strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.565 kb on - strand, within Pf6N2E2_2770 at 3258.566 kb on + strand, within Pf6N2E2_2770 at 3258.567 kb on - strand, within Pf6N2E2_2770 at 3258.567 kb on - strand, within Pf6N2E2_2770 at 3258.567 kb on - strand, within Pf6N2E2_2770
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM remove 3,256,420 + Pf6N2E2_2768 0.21 +0.4 3,256,420 + Pf6N2E2_2768 0.21 +1.6 3,256,420 + Pf6N2E2_2768 0.21 -0.6 3,256,420 + Pf6N2E2_2768 0.21 -0.0 3,256,420 + Pf6N2E2_2768 0.21 +0.9 3,256,420 + Pf6N2E2_2768 0.21 +1.4 3,256,421 - Pf6N2E2_2768 0.21 -0.9 3,256,421 - Pf6N2E2_2768 0.21 +0.0 3,256,421 - Pf6N2E2_2768 0.21 -0.1 3,256,421 - Pf6N2E2_2768 0.21 +3.2 3,256,421 - Pf6N2E2_2768 0.21 -0.6 3,256,421 - Pf6N2E2_2768 0.21 +0.5 3,256,421 - Pf6N2E2_2768 0.21 -0.6 3,256,421 - Pf6N2E2_2768 0.21 +1.7 3,256,421 - Pf6N2E2_2768 0.21 +2.6 3,256,421 - Pf6N2E2_2768 0.21 +0.1 3,256,450 + Pf6N2E2_2768 0.24 +0.8 3,256,450 + Pf6N2E2_2768 0.24 +0.2 3,256,450 + Pf6N2E2_2768 0.24 +1.4 3,256,594 + Pf6N2E2_2768 0.38 -0.3 3,256,595 - Pf6N2E2_2768 0.38 +1.2 3,256,595 - Pf6N2E2_2768 0.38 +0.8 3,256,649 + Pf6N2E2_2768 0.44 +1.4 3,256,649 + Pf6N2E2_2768 0.44 +0.5 3,256,775 - Pf6N2E2_2768 0.57 +0.4 3,256,775 - Pf6N2E2_2768 0.57 -0.2 3,256,775 - Pf6N2E2_2768 0.57 +0.4 3,256,829 + Pf6N2E2_2768 0.62 +2.3 3,256,829 + Pf6N2E2_2768 0.62 +1.3 3,256,829 + Pf6N2E2_2768 0.62 +1.3 3,256,829 + Pf6N2E2_2768 0.62 +0.4 3,256,829 + Pf6N2E2_2768 0.62 +2.0 3,256,830 - Pf6N2E2_2768 0.62 -0.3 3,256,830 - Pf6N2E2_2768 0.62 +0.8 3,256,830 - +1.0 3,256,830 - Pf6N2E2_2768 0.62 +1.5 3,256,830 - Pf6N2E2_2768 0.62 +0.0 3,256,966 + Pf6N2E2_2768 0.76 -0.4 3,256,966 + Pf6N2E2_2768 0.76 +1.4 3,256,967 - Pf6N2E2_2768 0.76 -1.0 3,256,999 + Pf6N2E2_2768 0.79 +2.3 3,256,999 + Pf6N2E2_2768 0.79 +1.2 3,257,000 - Pf6N2E2_2768 0.79 -0.4 3,257,029 + Pf6N2E2_2768 0.82 -0.6 3,257,029 + Pf6N2E2_2768 0.82 +1.2 3,257,030 - Pf6N2E2_2768 0.82 +0.6 3,257,143 + +2.6 3,257,143 + +1.1 3,257,143 + +0.9 3,257,143 + +1.1 3,257,143 + +0.8 3,257,144 - +1.6 3,257,144 - +1.0 3,257,144 - +0.6 3,257,144 - -0.7 3,257,144 - +1.0 3,257,144 - +0.5 3,257,144 - +2.1 3,257,144 - +0.7 3,257,144 - +1.1 3,257,144 - +0.0 3,257,144 - +0.2 3,257,144 - +0.0 3,257,144 - -0.6 3,257,144 - +2.0 3,257,144 - +0.8 3,257,144 - +1.5 3,257,167 + +0.2 3,257,167 + +1.9 3,257,167 + +0.6 3,257,167 + +1.3 3,257,168 - -0.3 3,257,168 - +1.4 3,257,256 + -0.4 3,257,256 + +0.7 3,257,256 + -1.1 3,257,256 + +0.0 3,257,257 - +1.0 3,257,279 - +0.3 3,257,354 + Pf6N2E2_2769 0.20 -0.6 3,257,420 + Pf6N2E2_2769 0.34 +1.8 3,257,420 + Pf6N2E2_2769 0.34 +1.4 3,257,420 + Pf6N2E2_2769 0.34 +2.0 3,257,420 + Pf6N2E2_2769 0.34 +1.3 3,257,420 + Pf6N2E2_2769 0.34 +0.3 3,257,421 - Pf6N2E2_2769 0.35 +1.0 3,257,421 - Pf6N2E2_2769 0.35 -0.4 3,257,453 - Pf6N2E2_2769 0.42 -1.6 3,257,501 - Pf6N2E2_2769 0.52 +0.9 3,257,598 - Pf6N2E2_2769 0.73 +1.4 3,257,654 + Pf6N2E2_2769 0.85 +1.4 3,257,654 + Pf6N2E2_2769 0.85 +1.4 3,257,655 - Pf6N2E2_2769 0.85 +0.0 3,257,724 - +1.0 3,257,739 + +1.7 3,257,739 + -0.3 3,257,739 + +0.3 3,257,775 + +1.0 3,257,775 + +0.4 3,257,775 + +3.0 3,257,931 + Pf6N2E2_2770 0.14 +0.0 3,257,931 + Pf6N2E2_2770 0.14 +0.2 3,257,932 - Pf6N2E2_2770 0.14 -1.0 3,257,947 - Pf6N2E2_2770 0.15 -0.4 3,257,957 + Pf6N2E2_2770 0.15 -0.6 3,257,957 + Pf6N2E2_2770 0.15 +0.4 3,257,957 + Pf6N2E2_2770 0.15 +0.0 3,257,957 + Pf6N2E2_2770 0.15 +1.1 3,257,957 + Pf6N2E2_2770 0.15 -0.4 3,257,958 - Pf6N2E2_2770 0.15 +1.3 3,257,958 - Pf6N2E2_2770 0.15 -0.3 3,257,958 - Pf6N2E2_2770 0.15 +0.8 3,257,958 - Pf6N2E2_2770 0.15 +0.7 3,257,958 - Pf6N2E2_2770 0.15 -0.1 3,258,168 + Pf6N2E2_2770 0.29 -0.4 3,258,169 - Pf6N2E2_2770 0.29 +1.4 3,258,564 + Pf6N2E2_2770 0.55 +0.7 3,258,564 + Pf6N2E2_2770 0.55 +0.7 3,258,564 + Pf6N2E2_2770 0.55 -1.6 3,258,564 + Pf6N2E2_2770 0.55 +0.6 3,258,564 + Pf6N2E2_2770 0.55 +1.7 3,258,565 - Pf6N2E2_2770 0.56 +0.0 3,258,565 - Pf6N2E2_2770 0.56 +0.4 3,258,565 - Pf6N2E2_2770 0.56 +0.0 3,258,565 - Pf6N2E2_2770 0.56 -0.7 3,258,565 - Pf6N2E2_2770 0.56 -0.1 3,258,565 - Pf6N2E2_2770 0.56 -0.1 3,258,565 - Pf6N2E2_2770 0.56 +0.2 3,258,565 - Pf6N2E2_2770 0.56 +0.4 3,258,565 - Pf6N2E2_2770 0.56 +1.3 3,258,565 - Pf6N2E2_2770 0.56 +0.8 3,258,566 + Pf6N2E2_2770 0.56 +0.2 3,258,567 - Pf6N2E2_2770 0.56 +0.9 3,258,567 - Pf6N2E2_2770 0.56 +0.8 3,258,567 - Pf6N2E2_2770 0.56 +2.5
Or see this region's nucleotide sequence