Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0144 and SMa0146 are separated by 5 nucleotides SMa0146 and SMa0148 are separated by 140 nucleotides SMa0148 and SMa0149 are separated by 184 nucleotides SMa0149 and SMa0150 are separated by 33 nucleotides
SMa0144: SMa0144 - hypothetical protein, at 79,504 to 80,289
SMa0144
SMa0146: SMa0146 - hypothetical protein, at 80,295 to 80,819
SMa0146
SMa0148: SMa0148 - hypothetical protein, at 80,960 to 81,673
SMa0148
SMa0149: SMa0149 - transcription elongation factor regulatory protein, at 81,858 to 82,340
SMa0149
SMa0150: SMa0150 - malonyl-CoA synthase, at 82,374 to 83,900
SMa0150
Position (kb) 80
81
82 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.378 kb on + strand, within SMa0146 at 80.379 kb on - strand, within SMa0146 at 80.464 kb on + strand, within SMa0146 at 80.465 kb on - strand, within SMa0146 at 80.465 kb on - strand, within SMa0146 at 80.540 kb on + strand, within SMa0146 at 80.541 kb on - strand, within SMa0146 at 80.658 kb on + strand, within SMa0146 at 80.658 kb on + strand, within SMa0146 at 80.855 kb on - strand at 80.942 kb on + strand at 80.943 kb on - strand at 80.943 kb on - strand at 80.943 kb on - strand at 81.101 kb on + strand, within SMa0148 at 81.110 kb on + strand, within SMa0148 at 81.111 kb on - strand, within SMa0148 at 81.179 kb on - strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.298 kb on - strand, within SMa0148 at 81.472 kb on + strand, within SMa0148 at 81.472 kb on + strand, within SMa0148 at 81.472 kb on + strand, within SMa0148 at 81.472 kb on + strand, within SMa0148 at 81.669 kb on - strand at 81.669 kb on - strand at 81.723 kb on + strand at 81.724 kb on - strand at 81.724 kb on - strand at 81.775 kb on + strand at 81.775 kb on + strand at 81.776 kb on - strand at 81.786 kb on + strand at 81.786 kb on + strand at 81.788 kb on + strand at 81.790 kb on + strand at 81.790 kb on + strand at 81.791 kb on - strand at 81.791 kb on - strand at 81.791 kb on - strand at 81.822 kb on + strand at 81.823 kb on - strand at 81.823 kb on - strand at 82.081 kb on - strand, within SMa0149 at 82.139 kb on - strand, within SMa0149 at 82.245 kb on + strand, within SMa0149 at 82.245 kb on + strand, within SMa0149 at 82.246 kb on - strand, within SMa0149 at 82.246 kb on - strand, within SMa0149 at 82.339 kb on - strand at 82.411 kb on + strand at 82.411 kb on + strand at 82.425 kb on + strand at 82.425 kb on + strand at 82.426 kb on - strand at 82.451 kb on + strand at 82.451 kb on + strand at 82.452 kb on - strand at 82.498 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 80,287 + -0.1 80,287 + -3.3 80,287 + -0.6 80,287 + -0.2 80,287 + -1.5 80,287 + -0.9 80,287 + +0.2 80,287 + +3.2 80,378 + SMa0146 0.16 -1.1 80,379 - SMa0146 0.16 -0.8 80,464 + SMa0146 0.32 +0.0 80,465 - SMa0146 0.32 +0.9 80,465 - SMa0146 0.32 -1.7 80,540 + SMa0146 0.47 -0.2 80,541 - SMa0146 0.47 -0.2 80,658 + SMa0146 0.69 +1.0 80,658 + SMa0146 0.69 -0.3 80,855 - -0.9 80,942 + -0.7 80,943 - -0.0 80,943 - +1.2 80,943 - +0.9 81,101 + SMa0148 0.20 -2.9 81,110 + SMa0148 0.21 -0.1 81,111 - SMa0148 0.21 -0.4 81,179 - SMa0148 0.31 -0.1 81,189 + SMa0148 0.32 -0.3 81,189 + SMa0148 0.32 -0.9 81,189 + SMa0148 0.32 -0.6 81,189 + SMa0148 0.32 -0.7 81,189 + SMa0148 0.32 +1.1 81,190 - SMa0148 0.32 -0.9 81,190 - SMa0148 0.32 -1.7 81,190 - SMa0148 0.32 +0.3 81,190 - SMa0148 0.32 -0.1 81,190 - SMa0148 0.32 -1.6 81,190 - SMa0148 0.32 -2.7 81,190 - SMa0148 0.32 -2.0 81,190 - SMa0148 0.32 -0.5 81,298 - SMa0148 0.47 +0.0 81,472 + SMa0148 0.72 +0.6 81,472 + SMa0148 0.72 +0.9 81,472 + SMa0148 0.72 -0.2 81,472 + SMa0148 0.72 -0.4 81,669 - -0.3 81,669 - -2.4 81,723 + +0.8 81,724 - +1.9 81,724 - +0.6 81,775 + -0.7 81,775 + -0.3 81,776 - +1.2 81,786 + -1.4 81,786 + -1.2 81,788 + +0.7 81,790 + -1.3 81,790 + -1.5 81,791 - +1.4 81,791 - -1.3 81,791 - +0.1 81,822 + +0.2 81,823 - -0.0 81,823 - +1.4 82,081 - SMa0149 0.46 +0.9 82,139 - SMa0149 0.58 +1.6 82,245 + SMa0149 0.80 +1.7 82,245 + SMa0149 0.80 +2.3 82,246 - SMa0149 0.80 +1.5 82,246 - SMa0149 0.80 -0.8 82,339 - -0.8 82,411 + +0.5 82,411 + +0.9 82,425 + -0.0 82,425 + +0.3 82,426 - -0.6 82,451 + +2.2 82,451 + -0.9 82,452 - +1.9 82,498 + -0.7
Or see this region's nucleotide sequence