Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20299 and SM_b20300 are separated by 336 nucleotides SM_b20300 and SM_b20301 overlap by 4 nucleotides
SM_b20299: SM_b20299 - N-acetylneuraminate lyase, at 305,696 to 306,595
_b20299
SM_b20300: SM_b20300 - adenylate cyclase, at 306,932 to 308,845
_b20300
SM_b20301: SM_b20301 - dehydgrogenase, at 308,842 to 309,972
_b20301
Position (kb) 306
307
308
309 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 306.017 kb on + strand, within SM_b20299 at 306.017 kb on + strand, within SM_b20299 at 306.021 kb on - strand, within SM_b20299 at 306.021 kb on - strand, within SM_b20299 at 306.096 kb on - strand, within SM_b20299 at 306.096 kb on - strand, within SM_b20299 at 306.097 kb on + strand, within SM_b20299 at 306.097 kb on + strand, within SM_b20299 at 306.098 kb on - strand, within SM_b20299 at 306.098 kb on - strand, within SM_b20299 at 306.181 kb on + strand, within SM_b20299 at 306.182 kb on - strand, within SM_b20299 at 306.182 kb on - strand, within SM_b20299 at 306.182 kb on - strand, within SM_b20299 at 306.242 kb on + strand, within SM_b20299 at 306.243 kb on - strand, within SM_b20299 at 306.243 kb on - strand, within SM_b20299 at 306.243 kb on - strand, within SM_b20299 at 306.312 kb on - strand, within SM_b20299 at 306.312 kb on - strand, within SM_b20299 at 306.314 kb on - strand, within SM_b20299 at 306.594 kb on - strand at 306.661 kb on - strand at 306.737 kb on + strand at 306.738 kb on - strand at 306.771 kb on + strand at 306.772 kb on - strand at 306.780 kb on - strand at 306.900 kb on - strand at 306.940 kb on + strand at 306.977 kb on + strand at 306.977 kb on + strand at 306.978 kb on - strand at 306.980 kb on - strand at 307.013 kb on + strand at 307.013 kb on + strand at 307.013 kb on + strand at 307.051 kb on - strand at 307.051 kb on - strand at 307.051 kb on - strand at 307.051 kb on - strand at 307.051 kb on - strand at 307.218 kb on + strand, within SM_b20300 at 307.218 kb on + strand, within SM_b20300 at 307.218 kb on + strand, within SM_b20300 at 307.218 kb on + strand, within SM_b20300 at 307.218 kb on + strand, within SM_b20300 at 307.219 kb on - strand, within SM_b20300 at 307.352 kb on + strand, within SM_b20300 at 307.545 kb on + strand, within SM_b20300 at 307.697 kb on - strand, within SM_b20300 at 307.697 kb on - strand, within SM_b20300 at 307.697 kb on - strand, within SM_b20300 at 307.699 kb on - strand, within SM_b20300 at 307.814 kb on + strand, within SM_b20300 at 307.815 kb on - strand, within SM_b20300 at 307.935 kb on + strand, within SM_b20300 at 307.935 kb on + strand, within SM_b20300 at 307.936 kb on - strand, within SM_b20300 at 307.936 kb on - strand, within SM_b20300 at 307.988 kb on + strand, within SM_b20300 at 307.988 kb on + strand, within SM_b20300 at 307.988 kb on + strand, within SM_b20300 at 307.988 kb on + strand, within SM_b20300 at 308.048 kb on + strand, within SM_b20300 at 308.048 kb on + strand, within SM_b20300 at 308.048 kb on + strand, within SM_b20300 at 308.079 kb on - strand, within SM_b20300 at 308.079 kb on - strand, within SM_b20300 at 308.192 kb on + strand, within SM_b20300 at 308.192 kb on + strand, within SM_b20300 at 308.193 kb on - strand, within SM_b20300 at 308.193 kb on - strand, within SM_b20300 at 308.193 kb on - strand, within SM_b20300 at 308.193 kb on - strand, within SM_b20300 at 308.507 kb on + strand, within SM_b20300 at 308.508 kb on - strand, within SM_b20300 at 308.510 kb on + strand, within SM_b20300 at 308.510 kb on + strand, within SM_b20300 at 308.510 kb on + strand, within SM_b20300 at 308.511 kb on - strand, within SM_b20300 at 308.511 kb on - strand, within SM_b20300 at 308.512 kb on + strand, within SM_b20300 at 308.512 kb on + strand, within SM_b20300 at 308.512 kb on + strand, within SM_b20300 at 308.512 kb on + strand, within SM_b20300 at 308.513 kb on - strand, within SM_b20300 at 308.513 kb on - strand, within SM_b20300 at 308.513 kb on - strand, within SM_b20300 at 308.516 kb on + strand, within SM_b20300 at 308.516 kb on + strand, within SM_b20300 at 308.516 kb on + strand, within SM_b20300 at 308.516 kb on + strand, within SM_b20300 at 308.538 kb on - strand, within SM_b20300 at 309.001 kb on - strand, within SM_b20301 at 309.007 kb on + strand, within SM_b20301 at 309.064 kb on - strand, within SM_b20301 at 309.065 kb on + strand, within SM_b20301 at 309.065 kb on + strand, within SM_b20301 at 309.065 kb on + strand, within SM_b20301 at 309.066 kb on - strand, within SM_b20301 at 309.066 kb on - strand, within SM_b20301 at 309.105 kb on + strand, within SM_b20301 at 309.162 kb on + strand, within SM_b20301 at 309.163 kb on - strand, within SM_b20301 at 309.164 kb on + strand, within SM_b20301 at 309.197 kb on + strand, within SM_b20301 at 309.218 kb on - strand, within SM_b20301 at 309.314 kb on + strand, within SM_b20301 at 309.315 kb on - strand, within SM_b20301 at 309.315 kb on - strand, within SM_b20301 at 309.463 kb on + strand, within SM_b20301 at 309.463 kb on + strand, within SM_b20301 at 309.463 kb on + strand, within SM_b20301 at 309.464 kb on - strand, within SM_b20301 at 309.638 kb on + strand, within SM_b20301 at 309.639 kb on - strand, within SM_b20301
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 306,017 + SM_b20299 0.36 +1.0 306,017 + SM_b20299 0.36 +1.0 306,021 - SM_b20299 0.36 -0.2 306,021 - SM_b20299 0.36 +0.0 306,096 - SM_b20299 0.44 -0.4 306,096 - SM_b20299 0.44 +0.7 306,097 + SM_b20299 0.45 -1.5 306,097 + SM_b20299 0.45 +0.4 306,098 - SM_b20299 0.45 +0.3 306,098 - SM_b20299 0.45 +0.0 306,181 + SM_b20299 0.54 -0.1 306,182 - SM_b20299 0.54 +0.6 306,182 - SM_b20299 0.54 +0.7 306,182 - SM_b20299 0.54 +0.5 306,242 + SM_b20299 0.61 +0.6 306,243 - SM_b20299 0.61 +1.2 306,243 - SM_b20299 0.61 -0.2 306,243 - SM_b20299 0.61 +1.0 306,312 - SM_b20299 0.68 +0.5 306,312 - SM_b20299 0.68 -0.0 306,314 - SM_b20299 0.69 +0.8 306,594 - +0.0 306,661 - -0.7 306,737 + +1.8 306,738 - +1.0 306,771 + -1.6 306,772 - +2.0 306,780 - -0.3 306,900 - +1.8 306,940 + -0.6 306,977 + +0.8 306,977 + +1.4 306,978 - +0.1 306,980 - -0.3 307,013 + +0.4 307,013 + +1.4 307,013 + -0.6 307,051 - -0.0 307,051 - -0.1 307,051 - -2.2 307,051 - -0.7 307,051 - +1.8 307,218 + SM_b20300 0.15 +0.3 307,218 + SM_b20300 0.15 -0.6 307,218 + SM_b20300 0.15 -2.5 307,218 + SM_b20300 0.15 -2.4 307,218 + SM_b20300 0.15 -0.2 307,219 - SM_b20300 0.15 -0.2 307,352 + SM_b20300 0.22 -1.8 307,545 + SM_b20300 0.32 -0.2 307,697 - SM_b20300 0.40 +0.4 307,697 - SM_b20300 0.40 -0.7 307,697 - SM_b20300 0.40 +0.2 307,699 - SM_b20300 0.40 +1.4 307,814 + SM_b20300 0.46 -1.4 307,815 - SM_b20300 0.46 +1.8 307,935 + SM_b20300 0.52 +0.3 307,935 + SM_b20300 0.52 +1.2 307,936 - SM_b20300 0.52 +1.5 307,936 - SM_b20300 0.52 -0.8 307,988 + SM_b20300 0.55 -3.2 307,988 + SM_b20300 0.55 -0.5 307,988 + SM_b20300 0.55 -0.3 307,988 + SM_b20300 0.55 +0.2 308,048 + SM_b20300 0.58 +0.3 308,048 + SM_b20300 0.58 -0.9 308,048 + SM_b20300 0.58 -1.2 308,079 - SM_b20300 0.60 +0.3 308,079 - SM_b20300 0.60 +0.8 308,192 + SM_b20300 0.66 +1.3 308,192 + SM_b20300 0.66 -0.3 308,193 - SM_b20300 0.66 -2.3 308,193 - SM_b20300 0.66 -0.3 308,193 - SM_b20300 0.66 +1.8 308,193 - SM_b20300 0.66 +0.8 308,507 + SM_b20300 0.82 -1.6 308,508 - SM_b20300 0.82 -0.7 308,510 + SM_b20300 0.82 -0.6 308,510 + SM_b20300 0.82 -0.3 308,510 + SM_b20300 0.82 -0.2 308,511 - SM_b20300 0.82 -0.3 308,511 - SM_b20300 0.82 -1.4 308,512 + SM_b20300 0.83 -2.0 308,512 + SM_b20300 0.83 +0.8 308,512 + SM_b20300 0.83 +1.2 308,512 + SM_b20300 0.83 -0.6 308,513 - SM_b20300 0.83 -2.1 308,513 - SM_b20300 0.83 +0.4 308,513 - SM_b20300 0.83 +0.9 308,516 + SM_b20300 0.83 +0.1 308,516 + SM_b20300 0.83 +2.3 308,516 + SM_b20300 0.83 -0.6 308,516 + SM_b20300 0.83 -1.1 308,538 - SM_b20300 0.84 +0.2 309,001 - SM_b20301 0.14 +1.3 309,007 + SM_b20301 0.15 -0.8 309,064 - SM_b20301 0.20 -1.5 309,065 + SM_b20301 0.20 +0.4 309,065 + SM_b20301 0.20 +0.3 309,065 + SM_b20301 0.20 -1.0 309,066 - SM_b20301 0.20 +0.1 309,066 - SM_b20301 0.20 +0.5 309,105 + SM_b20301 0.23 +1.1 309,162 + SM_b20301 0.28 +1.1 309,163 - SM_b20301 0.28 -0.6 309,164 + SM_b20301 0.28 +2.3 309,197 + SM_b20301 0.31 -0.2 309,218 - SM_b20301 0.33 -0.9 309,314 + SM_b20301 0.42 -1.7 309,315 - SM_b20301 0.42 +0.6 309,315 - SM_b20301 0.42 +1.4 309,463 + SM_b20301 0.55 +0.7 309,463 + SM_b20301 0.55 -0.5 309,463 + SM_b20301 0.55 +0.3 309,464 - SM_b20301 0.55 +1.3 309,638 + SM_b20301 0.70 +2.1 309,639 - SM_b20301 0.70 -0.4
Or see this region's nucleotide sequence