Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20112 and SM_b20113 are separated by 36 nucleotides SM_b20113 and SM_b20114 are separated by 2 nucleotides SM_b20114 and SM_b20115 are separated by 26 nucleotides
SM_b20112: SM_b20112 - hypothetical protein, at 123,785 to 124,672
_b20112
SM_b20113: SM_b20113 - dehydrogenase, at 124,709 to 125,641
_b20113
SM_b20114: SM_b20114 - hypothetical protein, at 125,644 to 126,369
_b20114
SM_b20115: SM_b20115 - dihydroxy-acid dehydratase, at 126,396 to 128,117
_b20115
Position (kb) 125
126
127 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 124.671 kb on - strand at 124.960 kb on - strand, within SM_b20113 at 125.171 kb on - strand, within SM_b20113 at 125.171 kb on - strand, within SM_b20113 at 125.171 kb on - strand, within SM_b20113 at 125.412 kb on + strand, within SM_b20113 at 125.465 kb on - strand, within SM_b20113 at 125.558 kb on - strand at 125.646 kb on - strand at 125.709 kb on - strand at 126.197 kb on + strand, within SM_b20114 at 126.198 kb on - strand, within SM_b20114 at 126.198 kb on - strand, within SM_b20114 at 126.208 kb on - strand, within SM_b20114 at 126.319 kb on + strand at 126.319 kb on + strand at 126.359 kb on + strand at 126.359 kb on + strand at 126.359 kb on + strand at 126.359 kb on + strand at 126.360 kb on - strand at 126.360 kb on - strand at 126.360 kb on - strand at 126.503 kb on - strand at 126.515 kb on - strand at 126.558 kb on + strand at 126.688 kb on + strand, within SM_b20115 at 126.773 kb on + strand, within SM_b20115 at 127.009 kb on + strand, within SM_b20115 at 127.010 kb on - strand, within SM_b20115 at 127.010 kb on - strand, within SM_b20115 at 127.162 kb on + strand, within SM_b20115 at 127.162 kb on + strand, within SM_b20115 at 127.163 kb on - strand, within SM_b20115 at 127.180 kb on + strand, within SM_b20115 at 127.181 kb on - strand, within SM_b20115 at 127.181 kb on - strand, within SM_b20115
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 124,671 - +3.7 124,960 - SM_b20113 0.27 +1.2 125,171 - SM_b20113 0.50 +0.9 125,171 - SM_b20113 0.50 -2.2 125,171 - SM_b20113 0.50 -0.9 125,412 + SM_b20113 0.75 -1.8 125,465 - SM_b20113 0.81 -0.1 125,558 - -1.5 125,646 - -0.9 125,709 - -0.2 126,197 + SM_b20114 0.76 -1.8 126,198 - SM_b20114 0.76 -2.2 126,198 - SM_b20114 0.76 -3.2 126,208 - SM_b20114 0.78 +0.5 126,319 + +0.2 126,319 + -1.0 126,359 + -1.4 126,359 + -0.4 126,359 + +0.0 126,359 + -0.1 126,360 - -0.8 126,360 - +0.3 126,360 - +0.9 126,503 - -1.0 126,515 - +1.7 126,558 + +0.2 126,688 + SM_b20115 0.17 -0.2 126,773 + SM_b20115 0.22 -0.9 127,009 + SM_b20115 0.36 -0.5 127,010 - SM_b20115 0.36 -2.6 127,010 - SM_b20115 0.36 -0.7 127,162 + SM_b20115 0.44 +0.6 127,162 + SM_b20115 0.44 -0.2 127,163 - SM_b20115 0.45 +0.9 127,180 + SM_b20115 0.46 -1.2 127,181 - SM_b20115 0.46 +0.2 127,181 - SM_b20115 0.46 +1.5
Or see this region's nucleotide sequence