Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_857 and Pf6N2E2_858 are separated by 805 nucleotides Pf6N2E2_858 and Pf6N2E2_859 are separated by 305 nucleotides
Pf6N2E2_857 - hypothetical protein, at 1,003,313 to 1,003,483
_857
Pf6N2E2_858 - L-Proline/Glycine betaine transporter ProP, at 1,004,289 to 1,005,731
_858
Pf6N2E2_859 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26), at 1,006,037 to 1,007,617
_859
Position (kb)
1004
1005
1006 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1003.296 kb on + strand at 1003.296 kb on + strand at 1003.296 kb on + strand at 1003.296 kb on + strand at 1003.297 kb on - strand at 1003.297 kb on - strand at 1003.297 kb on - strand at 1003.297 kb on - strand at 1003.297 kb on - strand at 1003.297 kb on - strand at 1003.304 kb on - strand at 1003.315 kb on - strand at 1003.315 kb on - strand at 1003.330 kb on - strand, within Pf6N2E2_857 at 1003.330 kb on - strand, within Pf6N2E2_857 at 1003.385 kb on + strand, within Pf6N2E2_857 at 1003.385 kb on + strand, within Pf6N2E2_857 at 1003.386 kb on - strand, within Pf6N2E2_857 at 1003.386 kb on - strand, within Pf6N2E2_857 at 1003.393 kb on + strand, within Pf6N2E2_857 at 1003.393 kb on + strand, within Pf6N2E2_857 at 1003.394 kb on - strand, within Pf6N2E2_857 at 1003.394 kb on - strand, within Pf6N2E2_857 at 1003.394 kb on - strand, within Pf6N2E2_857 at 1003.394 kb on - strand, within Pf6N2E2_857 at 1003.394 kb on - strand, within Pf6N2E2_857 at 1003.511 kb on + strand at 1003.512 kb on - strand at 1003.513 kb on + strand at 1003.513 kb on + strand at 1003.514 kb on - strand at 1003.596 kb on + strand at 1003.596 kb on + strand at 1003.597 kb on - strand at 1003.597 kb on - strand at 1003.597 kb on - strand at 1003.632 kb on - strand at 1003.632 kb on - strand at 1003.632 kb on - strand at 1003.736 kb on + strand at 1003.736 kb on + strand at 1003.739 kb on - strand at 1003.879 kb on + strand at 1003.880 kb on - strand at 1003.880 kb on - strand at 1004.000 kb on + strand at 1004.001 kb on - strand at 1004.001 kb on - strand at 1004.001 kb on - strand at 1004.050 kb on + strand at 1004.050 kb on + strand at 1004.050 kb on + strand at 1004.050 kb on + strand at 1004.051 kb on - strand at 1004.051 kb on - strand at 1004.051 kb on - strand at 1004.051 kb on - strand at 1004.103 kb on + strand at 1004.103 kb on + strand at 1004.103 kb on + strand at 1004.103 kb on + strand at 1004.103 kb on + strand at 1004.103 kb on + strand at 1004.103 kb on + strand at 1004.103 kb on + strand at 1004.103 kb on + strand at 1004.104 kb on - strand at 1004.104 kb on - strand at 1004.104 kb on - strand at 1004.104 kb on - strand at 1004.104 kb on - strand at 1004.104 kb on - strand at 1004.104 kb on - strand at 1004.104 kb on - strand at 1004.104 kb on - strand at 1004.105 kb on + strand at 1004.105 kb on + strand at 1004.105 kb on + strand at 1004.105 kb on + strand at 1004.105 kb on + strand at 1004.105 kb on + strand at 1004.105 kb on + strand at 1004.105 kb on + strand at 1004.105 kb on + strand at 1004.105 kb on + strand at 1004.106 kb on - strand at 1004.106 kb on - strand at 1004.106 kb on - strand at 1004.106 kb on - strand at 1004.106 kb on - strand at 1004.106 kb on - strand at 1004.106 kb on - strand at 1004.106 kb on - strand at 1004.106 kb on - strand at 1004.106 kb on - strand at 1004.106 kb on - strand at 1004.106 kb on - strand at 1004.106 kb on - strand at 1004.107 kb on + strand at 1004.107 kb on + strand at 1004.107 kb on + strand at 1004.107 kb on + strand at 1004.107 kb on + strand at 1004.107 kb on + strand at 1004.107 kb on + strand at 1004.107 kb on + strand at 1004.107 kb on + strand at 1004.107 kb on + strand at 1004.107 kb on + strand at 1004.107 kb on + strand at 1004.108 kb on - strand at 1004.108 kb on - strand at 1004.108 kb on - strand at 1004.146 kb on - strand at 1004.146 kb on - strand at 1004.159 kb on + strand at 1004.159 kb on + strand at 1004.216 kb on + strand at 1004.217 kb on - strand at 1004.380 kb on + strand at 1004.381 kb on - strand at 1004.454 kb on - strand, within Pf6N2E2_858 at 1004.456 kb on + strand, within Pf6N2E2_858 at 1004.456 kb on + strand, within Pf6N2E2_858 at 1004.456 kb on + strand, within Pf6N2E2_858 at 1004.456 kb on + strand, within Pf6N2E2_858 at 1004.456 kb on + strand, within Pf6N2E2_858 at 1004.457 kb on - strand, within Pf6N2E2_858 at 1004.457 kb on - strand, within Pf6N2E2_858 at 1004.457 kb on - strand, within Pf6N2E2_858 at 1004.457 kb on - strand, within Pf6N2E2_858 at 1004.457 kb on - strand, within Pf6N2E2_858 at 1004.457 kb on - strand, within Pf6N2E2_858 at 1004.459 kb on + strand, within Pf6N2E2_858 at 1004.460 kb on - strand, within Pf6N2E2_858 at 1004.548 kb on - strand, within Pf6N2E2_858 at 1004.586 kb on + strand, within Pf6N2E2_858 at 1004.586 kb on + strand, within Pf6N2E2_858 at 1004.586 kb on + strand, within Pf6N2E2_858 at 1004.587 kb on - strand, within Pf6N2E2_858 at 1004.587 kb on - strand, within Pf6N2E2_858 at 1004.587 kb on - strand, within Pf6N2E2_858 at 1004.628 kb on - strand, within Pf6N2E2_858 at 1004.628 kb on - strand, within Pf6N2E2_858 at 1004.677 kb on + strand, within Pf6N2E2_858 at 1004.677 kb on + strand, within Pf6N2E2_858 at 1004.678 kb on - strand, within Pf6N2E2_858 at 1004.703 kb on + strand, within Pf6N2E2_858 at 1004.704 kb on - strand, within Pf6N2E2_858 at 1004.723 kb on + strand, within Pf6N2E2_858 at 1004.723 kb on + strand, within Pf6N2E2_858 at 1004.724 kb on - strand, within Pf6N2E2_858 at 1004.724 kb on - strand, within Pf6N2E2_858 at 1004.724 kb on - strand, within Pf6N2E2_858 at 1004.724 kb on - strand, within Pf6N2E2_858 at 1004.724 kb on - strand, within Pf6N2E2_858 at 1004.724 kb on - strand, within Pf6N2E2_858 at 1004.724 kb on - strand, within Pf6N2E2_858 at 1004.763 kb on + strand, within Pf6N2E2_858 at 1004.764 kb on - strand, within Pf6N2E2_858 at 1004.764 kb on - strand, within Pf6N2E2_858 at 1004.856 kb on + strand, within Pf6N2E2_858 at 1004.856 kb on + strand, within Pf6N2E2_858 at 1004.856 kb on + strand, within Pf6N2E2_858 at 1004.856 kb on + strand, within Pf6N2E2_858 at 1004.858 kb on + strand, within Pf6N2E2_858 at 1004.858 kb on + strand, within Pf6N2E2_858 at 1004.858 kb on + strand, within Pf6N2E2_858 at 1004.858 kb on + strand, within Pf6N2E2_858 at 1004.858 kb on + strand, within Pf6N2E2_858 at 1004.859 kb on - strand, within Pf6N2E2_858 at 1004.859 kb on - strand, within Pf6N2E2_858 at 1004.859 kb on - strand, within Pf6N2E2_858 at 1004.859 kb on - strand, within Pf6N2E2_858 at 1004.886 kb on + strand, within Pf6N2E2_858 at 1004.929 kb on + strand, within Pf6N2E2_858 at 1004.929 kb on + strand, within Pf6N2E2_858 at 1004.929 kb on + strand, within Pf6N2E2_858 at 1004.930 kb on - strand, within Pf6N2E2_858 at 1004.930 kb on - strand, within Pf6N2E2_858 at 1004.995 kb on - strand, within Pf6N2E2_858 at 1005.036 kb on + strand, within Pf6N2E2_858 at 1005.036 kb on + strand, within Pf6N2E2_858 at 1005.037 kb on - strand, within Pf6N2E2_858 at 1005.361 kb on - strand, within Pf6N2E2_858 at 1005.383 kb on + strand, within Pf6N2E2_858 at 1005.384 kb on - strand, within Pf6N2E2_858 at 1005.397 kb on + strand, within Pf6N2E2_858 at 1005.397 kb on + strand, within Pf6N2E2_858 at 1005.397 kb on + strand, within Pf6N2E2_858 at 1005.398 kb on - strand, within Pf6N2E2_858 at 1005.398 kb on - strand, within Pf6N2E2_858 at 1005.398 kb on - strand, within Pf6N2E2_858 at 1005.398 kb on - strand, within Pf6N2E2_858 at 1005.402 kb on - strand, within Pf6N2E2_858 at 1005.452 kb on - strand, within Pf6N2E2_858 at 1005.524 kb on + strand, within Pf6N2E2_858 at 1005.524 kb on + strand, within Pf6N2E2_858 at 1005.524 kb on + strand, within Pf6N2E2_858 at 1005.524 kb on + strand, within Pf6N2E2_858 at 1005.525 kb on - strand, within Pf6N2E2_858 at 1005.526 kb on + strand, within Pf6N2E2_858 at 1005.527 kb on - strand, within Pf6N2E2_858 at 1005.606 kb on + strand at 1005.625 kb on + strand at 1005.625 kb on + strand at 1005.625 kb on + strand at 1005.625 kb on + strand at 1005.625 kb on + strand at 1005.626 kb on - strand at 1005.629 kb on + strand at 1005.630 kb on - strand at 1005.640 kb on + strand at 1005.640 kb on + strand at 1005.640 kb on + strand at 1005.643 kb on + strand at 1005.826 kb on - strand at 1005.919 kb on + strand at 1006.012 kb on + strand at 1006.030 kb on + strand at 1006.030 kb on + strand at 1006.031 kb on - strand at 1006.031 kb on - strand at 1006.031 kb on - strand at 1006.031 kb on - strand at 1006.038 kb on + strand at 1006.039 kb on - strand at 1006.039 kb on - strand at 1006.039 kb on - strand at 1006.131 kb on + strand at 1006.327 kb on + strand, within Pf6N2E2_859 at 1006.484 kb on + strand, within Pf6N2E2_859 at 1006.485 kb on - strand, within Pf6N2E2_859 at 1006.485 kb on - strand, within Pf6N2E2_859 at 1006.541 kb on + strand, within Pf6N2E2_859 at 1006.541 kb on + strand, within Pf6N2E2_859 at 1006.541 kb on + strand, within Pf6N2E2_859 at 1006.542 kb on - strand, within Pf6N2E2_859 at 1006.542 kb on - strand, within Pf6N2E2_859
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 1,003,296 + +0.4 1,003,296 + -2.8 1,003,296 + -0.7 1,003,296 + +0.6 1,003,297 - -2.2 1,003,297 - +0.3 1,003,297 - +0.6 1,003,297 - +0.2 1,003,297 - -0.4 1,003,297 - +0.6 1,003,304 - -2.1 1,003,315 - -1.4 1,003,315 - -0.1 1,003,330 - Pf6N2E2_857 0.10 -1.4 1,003,330 - Pf6N2E2_857 0.10 -0.6 1,003,385 + Pf6N2E2_857 0.42 +0.8 1,003,385 + Pf6N2E2_857 0.42 -1.5 1,003,386 - Pf6N2E2_857 0.43 -1.4 1,003,386 - Pf6N2E2_857 0.43 -0.9 1,003,393 + Pf6N2E2_857 0.47 -1.6 1,003,393 + Pf6N2E2_857 0.47 -0.0 1,003,394 - Pf6N2E2_857 0.47 +1.5 1,003,394 - Pf6N2E2_857 0.47 -0.1 1,003,394 - Pf6N2E2_857 0.47 +2.2 1,003,394 - Pf6N2E2_857 0.47 -0.1 1,003,394 - Pf6N2E2_857 0.47 +1.9 1,003,511 + +0.2 1,003,512 - -0.9 1,003,513 + -0.4 1,003,513 + -0.8 1,003,514 - -0.6 1,003,596 + -0.2 1,003,596 + +0.4 1,003,597 - +0.6 1,003,597 - -0.3 1,003,597 - +0.4 1,003,632 - +0.6 1,003,632 - +0.5 1,003,632 - -1.9 1,003,736 + +0.6 1,003,736 + +2.4 1,003,739 - +0.6 1,003,879 + +0.2 1,003,880 - -0.1 1,003,880 - +0.2 1,004,000 + +0.3 1,004,001 - -0.5 1,004,001 - -1.2 1,004,001 - -0.4 1,004,050 + -0.0 1,004,050 + +0.1 1,004,050 + +1.4 1,004,050 + +0.6 1,004,051 - +1.4 1,004,051 - -0.4 1,004,051 - -0.2 1,004,051 - +0.6 1,004,103 + +0.2 1,004,103 + +0.8 1,004,103 + +0.3 1,004,103 + +0.3 1,004,103 + +0.6 1,004,103 + -0.1 1,004,103 + +0.9 1,004,103 + +0.7 1,004,103 + +0.9 1,004,104 - +0.1 1,004,104 - -0.5 1,004,104 - +0.2 1,004,104 - -0.2 1,004,104 - +2.0 1,004,104 - +0.1 1,004,104 - +0.5 1,004,104 - -0.4 1,004,104 - -1.7 1,004,105 + -0.4 1,004,105 + +3.2 1,004,105 + +1.2 1,004,105 + +0.4 1,004,105 + -0.9 1,004,105 + -0.4 1,004,105 + -0.6 1,004,105 + -0.4 1,004,105 + -0.1 1,004,105 + -1.5 1,004,106 - +0.6 1,004,106 - +0.6 1,004,106 - -0.7 1,004,106 - -0.6 1,004,106 - -1.1 1,004,106 - -0.5 1,004,106 - -0.1 1,004,106 - -0.4 1,004,106 - -0.0 1,004,106 - -2.4 1,004,106 - -0.2 1,004,106 - +0.2 1,004,106 - -0.4 1,004,107 + +0.3 1,004,107 + -0.9 1,004,107 + +0.1 1,004,107 + -0.4 1,004,107 + -0.7 1,004,107 + -0.7 1,004,107 + +0.4 1,004,107 + -0.3 1,004,107 + +0.6 1,004,107 + -0.7 1,004,107 + -1.4 1,004,107 + -0.6 1,004,108 - -1.9 1,004,108 - +0.6 1,004,108 - -0.7 1,004,146 - -0.1 1,004,146 - +0.4 1,004,159 + -0.2 1,004,159 + -0.2 1,004,216 + -0.1 1,004,217 - -1.5 1,004,380 + -0.1 1,004,381 - -1.9 1,004,454 - Pf6N2E2_858 0.11 -1.1 1,004,456 + Pf6N2E2_858 0.12 +0.1 1,004,456 + Pf6N2E2_858 0.12 +1.6 1,004,456 + Pf6N2E2_858 0.12 +0.6 1,004,456 + Pf6N2E2_858 0.12 -1.9 1,004,456 + Pf6N2E2_858 0.12 -0.7 1,004,457 - Pf6N2E2_858 0.12 -0.1 1,004,457 - Pf6N2E2_858 0.12 -0.4 1,004,457 - Pf6N2E2_858 0.12 -1.2 1,004,457 - Pf6N2E2_858 0.12 -1.2 1,004,457 - Pf6N2E2_858 0.12 -0.4 1,004,457 - Pf6N2E2_858 0.12 -0.3 1,004,459 + Pf6N2E2_858 0.12 -0.1 1,004,460 - Pf6N2E2_858 0.12 +0.3 1,004,548 - Pf6N2E2_858 0.18 -2.4 1,004,586 + Pf6N2E2_858 0.21 +0.6 1,004,586 + Pf6N2E2_858 0.21 +0.6 1,004,586 + Pf6N2E2_858 0.21 +0.6 1,004,587 - Pf6N2E2_858 0.21 -1.2 1,004,587 - Pf6N2E2_858 0.21 +0.6 1,004,587 - Pf6N2E2_858 0.21 -0.4 1,004,628 - Pf6N2E2_858 0.23 -0.9 1,004,628 - Pf6N2E2_858 0.23 +0.6 1,004,677 + Pf6N2E2_858 0.27 -0.8 1,004,677 + Pf6N2E2_858 0.27 -0.2 1,004,678 - Pf6N2E2_858 0.27 -1.9 1,004,703 + Pf6N2E2_858 0.29 -0.8 1,004,704 - Pf6N2E2_858 0.29 -0.4 1,004,723 + Pf6N2E2_858 0.30 -1.9 1,004,723 + Pf6N2E2_858 0.30 +0.2 1,004,724 - Pf6N2E2_858 0.30 -0.9 1,004,724 - Pf6N2E2_858 0.30 -0.4 1,004,724 - Pf6N2E2_858 0.30 -0.4 1,004,724 - Pf6N2E2_858 0.30 -0.4 1,004,724 - Pf6N2E2_858 0.30 +0.6 1,004,724 - Pf6N2E2_858 0.30 -0.4 1,004,724 - Pf6N2E2_858 0.30 -0.9 1,004,763 + Pf6N2E2_858 0.33 +0.5 1,004,764 - Pf6N2E2_858 0.33 +0.6 1,004,764 - Pf6N2E2_858 0.33 -0.9 1,004,856 + Pf6N2E2_858 0.39 +0.6 1,004,856 + Pf6N2E2_858 0.39 +0.6 1,004,856 + Pf6N2E2_858 0.39 +0.5 1,004,856 + Pf6N2E2_858 0.39 +0.5 1,004,858 + Pf6N2E2_858 0.39 -0.4 1,004,858 + Pf6N2E2_858 0.39 -2.2 1,004,858 + Pf6N2E2_858 0.39 -0.2 1,004,858 + Pf6N2E2_858 0.39 +0.6 1,004,858 + Pf6N2E2_858 0.39 -1.9 1,004,859 - Pf6N2E2_858 0.40 +0.6 1,004,859 - Pf6N2E2_858 0.40 +1.2 1,004,859 - Pf6N2E2_858 0.40 -0.6 1,004,859 - Pf6N2E2_858 0.40 -0.4 1,004,886 + Pf6N2E2_858 0.41 -1.9 1,004,929 + Pf6N2E2_858 0.44 -0.6 1,004,929 + Pf6N2E2_858 0.44 -0.4 1,004,929 + Pf6N2E2_858 0.44 -0.4 1,004,930 - Pf6N2E2_858 0.44 -0.4 1,004,930 - Pf6N2E2_858 0.44 -0.4 1,004,995 - Pf6N2E2_858 0.49 +0.4 1,005,036 + Pf6N2E2_858 0.52 +0.2 1,005,036 + Pf6N2E2_858 0.52 +0.3 1,005,037 - Pf6N2E2_858 0.52 +0.2 1,005,361 - Pf6N2E2_858 0.74 +1.1 1,005,383 + Pf6N2E2_858 0.76 -0.4 1,005,384 - Pf6N2E2_858 0.76 +2.6 1,005,397 + Pf6N2E2_858 0.77 +0.1 1,005,397 + Pf6N2E2_858 0.77 -0.4 1,005,397 + Pf6N2E2_858 0.77 -0.9 1,005,398 - Pf6N2E2_858 0.77 +0.3 1,005,398 - Pf6N2E2_858 0.77 -1.2 1,005,398 - Pf6N2E2_858 0.77 +2.2 1,005,398 - Pf6N2E2_858 0.77 +1.1 1,005,402 - Pf6N2E2_858 0.77 +0.6 1,005,452 - Pf6N2E2_858 0.81 +2.6 1,005,524 + Pf6N2E2_858 0.86 -0.4 1,005,524 + Pf6N2E2_858 0.86 +0.2 1,005,524 + Pf6N2E2_858 0.86 +2.2 1,005,524 + Pf6N2E2_858 0.86 -0.4 1,005,525 - Pf6N2E2_858 0.86 -3.2 1,005,526 + Pf6N2E2_858 0.86 +0.6 1,005,527 - Pf6N2E2_858 0.86 +0.1 1,005,606 + +2.0 1,005,625 + -0.4 1,005,625 + -0.4 1,005,625 + +0.3 1,005,625 + -0.4 1,005,625 + +2.2 1,005,626 - +0.1 1,005,629 + -0.9 1,005,630 - -1.9 1,005,640 + -0.6 1,005,640 + +0.9 1,005,640 + -0.9 1,005,643 + +1.4 1,005,826 - +0.1 1,005,919 + +0.2 1,006,012 + -0.4 1,006,030 + -1.2 1,006,030 + +0.5 1,006,031 - -0.2 1,006,031 - +1.2 1,006,031 - +0.6 1,006,031 - -0.7 1,006,038 + -1.1 1,006,039 - -0.8 1,006,039 - -0.8 1,006,039 - +0.6 1,006,131 + +1.4 1,006,327 + Pf6N2E2_859 0.18 -1.2 1,006,484 + Pf6N2E2_859 0.28 +2.0 1,006,485 - Pf6N2E2_859 0.28 +1.6 1,006,485 - Pf6N2E2_859 0.28 -0.7 1,006,541 + Pf6N2E2_859 0.32 +1.6 1,006,541 + Pf6N2E2_859 0.32 -0.5 1,006,541 + Pf6N2E2_859 0.32 -0.9 1,006,542 - Pf6N2E2_859 0.32 +0.6 1,006,542 - Pf6N2E2_859 0.32 +2.0
Or see this region's nucleotide sequence