Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_671 and Pf6N2E2_672 are separated by 51 nucleotides Pf6N2E2_672 and Pf6N2E2_673 overlap by 8 nucleotides Pf6N2E2_673 and Pf6N2E2_674 are separated by 12 nucleotides Pf6N2E2_674 and Pf6N2E2_675 overlap by 8 nucleotides
Pf6N2E2_671 - 2-methylaconitate racemase, at 776,215 to 777,243
_671
Pf6N2E2_672 - ABC-type polar amino acid transport system, ATPase component, at 777,295 to 778,017
_672
Pf6N2E2_673 - Probable permease of ABC transporter, at 778,010 to 778,660
_673
Pf6N2E2_674 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 778,673 to 779,338
_674
Pf6N2E2_675 - 2-Methylcitrate dehydratase AcnD, at 779,331 to 781,106
_675
Position (kb) 778
779 Strain fitness (log2 ratio) -2
-1
0
1 at 777.217 kb on + strand at 777.217 kb on + strand at 777.218 kb on - strand at 777.218 kb on - strand at 777.494 kb on - strand, within Pf6N2E2_672 at 777.548 kb on + strand, within Pf6N2E2_672 at 777.548 kb on + strand, within Pf6N2E2_672 at 777.549 kb on - strand, within Pf6N2E2_672 at 778.078 kb on - strand, within Pf6N2E2_673 at 778.078 kb on - strand, within Pf6N2E2_673 at 778.081 kb on - strand, within Pf6N2E2_673 at 778.282 kb on - strand, within Pf6N2E2_673 at 778.282 kb on - strand, within Pf6N2E2_673 at 778.375 kb on - strand, within Pf6N2E2_673 at 778.623 kb on + strand at 778.624 kb on - strand at 778.651 kb on + strand at 778.651 kb on + strand at 778.652 kb on - strand at 778.740 kb on + strand, within Pf6N2E2_674 at 778.741 kb on - strand, within Pf6N2E2_674 at 778.741 kb on - strand, within Pf6N2E2_674 at 778.741 kb on - strand, within Pf6N2E2_674 at 778.741 kb on - strand, within Pf6N2E2_674 at 778.765 kb on - strand, within Pf6N2E2_674 at 778.765 kb on - strand, within Pf6N2E2_674 at 778.794 kb on + strand, within Pf6N2E2_674 at 778.795 kb on - strand, within Pf6N2E2_674 at 778.891 kb on - strand, within Pf6N2E2_674 at 778.891 kb on - strand, within Pf6N2E2_674 at 779.106 kb on + strand, within Pf6N2E2_674 at 779.107 kb on - strand, within Pf6N2E2_674 at 779.115 kb on + strand, within Pf6N2E2_674 at 779.115 kb on + strand, within Pf6N2E2_674 at 779.116 kb on - strand, within Pf6N2E2_674 at 779.116 kb on - strand, within Pf6N2E2_674 at 779.204 kb on + strand, within Pf6N2E2_674 at 779.228 kb on + strand, within Pf6N2E2_674 at 779.229 kb on - strand, within Pf6N2E2_674 at 779.328 kb on + strand at 779.329 kb on - strand at 779.329 kb on - strand at 779.471 kb on - strand at 779.471 kb on - strand at 779.480 kb on - strand at 779.480 kb on - strand at 779.480 kb on - strand at 779.606 kb on + strand, within Pf6N2E2_675 at 779.656 kb on + strand, within Pf6N2E2_675 at 779.656 kb on + strand, within Pf6N2E2_675 at 779.656 kb on + strand, within Pf6N2E2_675 at 779.656 kb on + strand, within Pf6N2E2_675 at 779.657 kb on - strand, within Pf6N2E2_675
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 777,217 + +0.4 777,217 + -0.3 777,218 - -1.2 777,218 - -1.3 777,494 - Pf6N2E2_672 0.28 +0.7 777,548 + Pf6N2E2_672 0.35 +1.0 777,548 + Pf6N2E2_672 0.35 -1.3 777,549 - Pf6N2E2_672 0.35 +0.1 778,078 - Pf6N2E2_673 0.10 -1.3 778,078 - Pf6N2E2_673 0.10 -1.1 778,081 - Pf6N2E2_673 0.11 +0.7 778,282 - Pf6N2E2_673 0.42 -0.7 778,282 - Pf6N2E2_673 0.42 -0.9 778,375 - Pf6N2E2_673 0.56 -0.5 778,623 + +0.1 778,624 - -0.3 778,651 + +0.7 778,651 + +0.3 778,652 - -0.7 778,740 + Pf6N2E2_674 0.10 -0.1 778,741 - Pf6N2E2_674 0.10 +0.7 778,741 - Pf6N2E2_674 0.10 -0.1 778,741 - Pf6N2E2_674 0.10 -2.1 778,741 - Pf6N2E2_674 0.10 -1.2 778,765 - Pf6N2E2_674 0.14 -0.6 778,765 - Pf6N2E2_674 0.14 +0.2 778,794 + Pf6N2E2_674 0.18 +0.9 778,795 - Pf6N2E2_674 0.18 -0.8 778,891 - Pf6N2E2_674 0.33 -0.3 778,891 - Pf6N2E2_674 0.33 +0.1 779,106 + Pf6N2E2_674 0.65 -0.2 779,107 - Pf6N2E2_674 0.65 -2.1 779,115 + Pf6N2E2_674 0.66 -1.5 779,115 + Pf6N2E2_674 0.66 +0.6 779,116 - Pf6N2E2_674 0.67 -0.3 779,116 - Pf6N2E2_674 0.67 +0.2 779,204 + Pf6N2E2_674 0.80 -1.8 779,228 + Pf6N2E2_674 0.83 -1.7 779,229 - Pf6N2E2_674 0.83 -0.3 779,328 + +0.7 779,329 - +0.1 779,329 - -0.6 779,471 - +0.7 779,471 - +0.7 779,480 - +0.1 779,480 - -0.9 779,480 - -0.3 779,606 + Pf6N2E2_675 0.15 -0.9 779,656 + Pf6N2E2_675 0.18 -1.3 779,656 + Pf6N2E2_675 0.18 -2.7 779,656 + Pf6N2E2_675 0.18 -0.7 779,656 + Pf6N2E2_675 0.18 -0.1 779,657 - Pf6N2E2_675 0.18 -0.1
Or see this region's nucleotide sequence