Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1482 and Pf6N2E2_1483 are separated by 192 nucleotides Pf6N2E2_1483 and Pf6N2E2_1484 are separated by 28 nucleotides Pf6N2E2_1484 and Pf6N2E2_1485 are separated by 113 nucleotides
Pf6N2E2_1482 - Probable periplasmic spermidine/putrescine-binding protein, at 1,731,787 to 1,732,890
_1482
Pf6N2E2_1483 - transcriptional regulator, LuxR family, at 1,733,083 to 1,733,820
_1483
Pf6N2E2_1484 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 1,733,849 to 1,734,778
_1484
Pf6N2E2_1485 - Transcriptional regulator, at 1,734,892 to 1,735,776
_1485
Position (kb)
1733
1734
1735 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1732.948 kb on + strand at 1732.951 kb on - strand at 1732.968 kb on - strand at 1732.970 kb on + strand at 1732.971 kb on - strand at 1732.984 kb on + strand at 1732.984 kb on + strand at 1732.984 kb on + strand at 1732.985 kb on - strand at 1732.989 kb on - strand at 1733.015 kb on + strand at 1733.487 kb on - strand, within Pf6N2E2_1483 at 1733.700 kb on - strand, within Pf6N2E2_1483 at 1733.957 kb on - strand, within Pf6N2E2_1484 at 1734.050 kb on + strand, within Pf6N2E2_1484 at 1734.050 kb on + strand, within Pf6N2E2_1484 at 1734.050 kb on + strand, within Pf6N2E2_1484 at 1734.050 kb on + strand, within Pf6N2E2_1484 at 1734.051 kb on - strand, within Pf6N2E2_1484 at 1734.051 kb on - strand, within Pf6N2E2_1484 at 1734.051 kb on - strand, within Pf6N2E2_1484 at 1734.259 kb on - strand, within Pf6N2E2_1484 at 1734.501 kb on + strand, within Pf6N2E2_1484 at 1734.761 kb on + strand at 1734.761 kb on + strand at 1734.761 kb on + strand at 1734.761 kb on + strand at 1734.762 kb on - strand at 1734.762 kb on - strand at 1734.762 kb on - strand at 1735.094 kb on + strand, within Pf6N2E2_1485 at 1735.094 kb on + strand, within Pf6N2E2_1485 at 1735.095 kb on - strand, within Pf6N2E2_1485 at 1735.095 kb on - strand, within Pf6N2E2_1485 at 1735.112 kb on + strand, within Pf6N2E2_1485 at 1735.112 kb on + strand, within Pf6N2E2_1485 at 1735.113 kb on - strand, within Pf6N2E2_1485 at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.265 kb on + strand at 1735.265 kb on + strand, within Pf6N2E2_1485 at 1735.564 kb on + strand, within Pf6N2E2_1485 at 1735.565 kb on - strand, within Pf6N2E2_1485 at 1735.600 kb on + strand, within Pf6N2E2_1485
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 1,732,948 + -0.4 1,732,951 - +0.6 1,732,968 - +0.6 1,732,970 + +0.2 1,732,971 - +0.0 1,732,984 + +0.6 1,732,984 + +0.6 1,732,984 + -0.5 1,732,985 - -0.7 1,732,989 - -1.0 1,733,015 + +0.8 1,733,487 - Pf6N2E2_1483 0.55 -2.9 1,733,700 - Pf6N2E2_1483 0.84 -0.1 1,733,957 - Pf6N2E2_1484 0.12 -1.0 1,734,050 + Pf6N2E2_1484 0.22 -1.0 1,734,050 + Pf6N2E2_1484 0.22 -0.4 1,734,050 + Pf6N2E2_1484 0.22 -0.8 1,734,050 + Pf6N2E2_1484 0.22 +0.6 1,734,051 - Pf6N2E2_1484 0.22 +0.6 1,734,051 - Pf6N2E2_1484 0.22 -1.1 1,734,051 - Pf6N2E2_1484 0.22 -0.4 1,734,259 - Pf6N2E2_1484 0.44 -0.4 1,734,501 + Pf6N2E2_1484 0.70 +0.0 1,734,761 + -0.6 1,734,761 + -0.7 1,734,761 + -0.1 1,734,761 + +0.0 1,734,762 - -1.4 1,734,762 - +0.6 1,734,762 - +0.6 1,735,094 + Pf6N2E2_1485 0.23 -1.1 1,735,094 + Pf6N2E2_1485 0.23 -0.7 1,735,095 - Pf6N2E2_1485 0.23 +1.0 1,735,095 - Pf6N2E2_1485 0.23 +0.9 1,735,112 + Pf6N2E2_1485 0.25 +0.6 1,735,112 + Pf6N2E2_1485 0.25 -0.3 1,735,113 - Pf6N2E2_1485 0.25 +1.2 1,735,265 + Pf6N2E2_1485 0.42 -0.5 1,735,265 + Pf6N2E2_1485 0.42 -0.4 1,735,265 + Pf6N2E2_1485 0.42 +1.2 1,735,265 + Pf6N2E2_1485 0.42 -1.6 1,735,265 + +0.6 1,735,265 + Pf6N2E2_1485 0.42 +0.1 1,735,564 + Pf6N2E2_1485 0.76 +1.6 1,735,565 - Pf6N2E2_1485 0.76 -1.4 1,735,600 + Pf6N2E2_1485 0.80 -0.4
Or see this region's nucleotide sequence