Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02038 and SMc02039 are separated by 17 nucleotides SMc02039 and SMc02040 overlap by 403 nucleotides SMc02040 and SMc02041 are separated by 1 nucleotides SMc02041 and SMc02042 are separated by 30 nucleotides SMc02042 and SMc02043 overlap by 4 nucleotides
SMc02038: SMc02038 - glycerol dehydrogenase, at 2,744,318 to 2,745,412
SMc02038
SMc02039: SMc02039 - oxidoreductase, at 2,745,430 to 2,746,128
SMc02039
SMc02040: SMc02040 - oxidoreductase, at 2,745,726 to 2,746,157
SMc02040
SMc02041: SMc02041 - short chain dehydrogenase, at 2,746,159 to 2,746,923
SMc02041
SMc02042: SMc02042 - glucose-6-phosphate isomerase, at 2,746,954 to 2,747,598
SMc02042
SMc02043: SMc02043 - KHG/KDPG aldolase, at 2,747,595 to 2,748,248
SMc02043
Position (kb)
2746
2747 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2745.211 kb on - strand, within SMc02038 at 2745.211 kb on - strand, within SMc02038 at 2745.211 kb on - strand, within SMc02038 at 2745.329 kb on + strand at 2745.329 kb on + strand at 2745.329 kb on + strand at 2745.330 kb on - strand at 2745.330 kb on - strand at 2745.330 kb on - strand at 2745.355 kb on - strand at 2745.518 kb on - strand, within SMc02039 at 2745.607 kb on + strand, within SMc02039 at 2745.607 kb on + strand, within SMc02039 at 2745.607 kb on + strand, within SMc02039 at 2745.608 kb on - strand, within SMc02039 at 2745.608 kb on - strand, within SMc02039 at 2745.608 kb on - strand, within SMc02039 at 2745.933 kb on + strand at 2745.934 kb on - strand at 2746.092 kb on + strand at 2746.477 kb on + strand, within SMc02041 at 2746.844 kb on + strand, within SMc02041 at 2746.844 kb on + strand, within SMc02041 at 2746.845 kb on - strand, within SMc02041 at 2746.849 kb on + strand at 2746.849 kb on + strand at 2746.940 kb on + strand at 2746.941 kb on - strand at 2746.941 kb on - strand at 2746.941 kb on - strand at 2746.964 kb on + strand at 2746.964 kb on + strand at 2746.964 kb on + strand at 2747.062 kb on + strand, within SMc02042 at 2747.109 kb on + strand, within SMc02042 at 2747.110 kb on - strand, within SMc02042 at 2747.119 kb on + strand, within SMc02042 at 2747.120 kb on - strand, within SMc02042 at 2747.209 kb on + strand, within SMc02042 at 2747.209 kb on + strand, within SMc02042 at 2747.260 kb on + strand, within SMc02042 at 2747.260 kb on + strand, within SMc02042 at 2747.261 kb on - strand, within SMc02042 at 2747.263 kb on + strand, within SMc02042 at 2747.264 kb on - strand, within SMc02042 at 2747.350 kb on + strand, within SMc02042 at 2747.351 kb on - strand, within SMc02042 at 2747.363 kb on - strand, within SMc02042 at 2747.385 kb on + strand, within SMc02042 at 2747.444 kb on - strand, within SMc02042 at 2747.566 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,745,211 - SMc02038 0.82 +0.5 2,745,211 - SMc02038 0.82 -0.9 2,745,211 - SMc02038 0.82 -0.5 2,745,329 + +0.8 2,745,329 + +0.9 2,745,329 + +0.3 2,745,330 - +0.9 2,745,330 - -0.7 2,745,330 - -0.5 2,745,355 - -0.4 2,745,518 - SMc02039 0.13 +0.0 2,745,607 + SMc02039 0.25 +0.4 2,745,607 + SMc02039 0.25 -0.9 2,745,607 + SMc02039 0.25 -0.6 2,745,608 - SMc02039 0.25 -1.3 2,745,608 - SMc02039 0.25 -2.0 2,745,608 - SMc02039 0.25 -0.1 2,745,933 + +0.0 2,745,934 - -1.1 2,746,092 + -1.1 2,746,477 + SMc02041 0.42 +1.4 2,746,844 + SMc02041 0.90 +0.2 2,746,844 + SMc02041 0.90 +0.7 2,746,845 - SMc02041 0.90 +0.5 2,746,849 + -0.3 2,746,849 + -0.1 2,746,940 + -0.6 2,746,941 - +0.9 2,746,941 - +0.6 2,746,941 - +0.2 2,746,964 + +1.7 2,746,964 + +0.6 2,746,964 + -0.8 2,747,062 + SMc02042 0.17 -1.1 2,747,109 + SMc02042 0.24 +0.0 2,747,110 - SMc02042 0.24 -1.3 2,747,119 + SMc02042 0.26 -0.2 2,747,120 - SMc02042 0.26 -0.2 2,747,209 + SMc02042 0.40 -0.1 2,747,209 + SMc02042 0.40 +0.2 2,747,260 + SMc02042 0.47 +0.6 2,747,260 + SMc02042 0.47 -0.4 2,747,261 - SMc02042 0.48 +2.5 2,747,263 + SMc02042 0.48 -0.1 2,747,264 - SMc02042 0.48 -0.9 2,747,350 + SMc02042 0.61 -0.6 2,747,351 - SMc02042 0.62 -0.9 2,747,363 - SMc02042 0.63 +0.7 2,747,385 + SMc02042 0.67 -2.7 2,747,444 - SMc02042 0.76 -1.7 2,747,566 + -0.6
Or see this region's nucleotide sequence