Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1844 and SMa1846 are separated by 9 nucleotides SMa1846 and SMa1848 are separated by 34 nucleotides
SMa1844: SMa1844 - aldehyde, at 1,045,137 to 1,046,600
SMa1844
SMa1846: SMa1846 - 3-isopropylmalate dehydrogenase, at 1,046,610 to 1,047,653
SMa1846
SMa1848: SMa1848 - GabD5 succinate semialdehyde dehdyrogenase, at 1,047,688 to 1,049,142
SMa1848
Position (kb)
1046
1047
1048 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1045.716 kb on + strand, within SMa1844 at 1045.717 kb on - strand, within SMa1844 at 1045.827 kb on - strand, within SMa1844 at 1045.827 kb on - strand, within SMa1844 at 1046.245 kb on - strand, within SMa1844 at 1046.383 kb on - strand, within SMa1844 at 1046.476 kb on - strand at 1046.514 kb on + strand at 1046.514 kb on + strand at 1046.514 kb on + strand at 1046.556 kb on + strand at 1046.556 kb on + strand at 1046.726 kb on + strand, within SMa1846 at 1046.754 kb on + strand, within SMa1846 at 1046.755 kb on - strand, within SMa1846 at 1046.755 kb on - strand, within SMa1846 at 1046.755 kb on - strand, within SMa1846 at 1046.756 kb on + strand, within SMa1846 at 1046.911 kb on - strand, within SMa1846 at 1046.911 kb on - strand, within SMa1846 at 1046.931 kb on - strand, within SMa1846 at 1046.931 kb on - strand, within SMa1846 at 1047.229 kb on - strand, within SMa1846 at 1047.252 kb on + strand, within SMa1846 at 1047.252 kb on + strand, within SMa1846 at 1047.253 kb on - strand, within SMa1846 at 1047.253 kb on - strand, within SMa1846 at 1047.253 kb on - strand, within SMa1846 at 1047.253 kb on - strand, within SMa1846 at 1047.456 kb on - strand, within SMa1846 at 1047.682 kb on - strand at 1048.308 kb on + strand, within SMa1848 at 1048.591 kb on + strand, within SMa1848 at 1048.592 kb on - strand, within SMa1848
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,045,716 + SMa1844 0.40 -0.6 1,045,717 - SMa1844 0.40 -0.2 1,045,827 - SMa1844 0.47 -0.3 1,045,827 - SMa1844 0.47 -0.9 1,046,245 - SMa1844 0.76 +0.5 1,046,383 - SMa1844 0.85 -0.1 1,046,476 - -0.7 1,046,514 + +0.4 1,046,514 + -0.1 1,046,514 + +0.2 1,046,556 + -0.9 1,046,556 + +1.9 1,046,726 + SMa1846 0.11 +0.8 1,046,754 + SMa1846 0.14 -1.0 1,046,755 - SMa1846 0.14 +2.0 1,046,755 - SMa1846 0.14 -0.2 1,046,755 - SMa1846 0.14 +0.2 1,046,756 + SMa1846 0.14 +0.1 1,046,911 - SMa1846 0.29 +1.4 1,046,911 - SMa1846 0.29 -0.3 1,046,931 - SMa1846 0.31 -1.8 1,046,931 - SMa1846 0.31 +1.1 1,047,229 - SMa1846 0.59 -0.2 1,047,252 + SMa1846 0.61 +0.2 1,047,252 + SMa1846 0.61 +0.8 1,047,253 - SMa1846 0.62 +2.1 1,047,253 - SMa1846 0.62 +0.6 1,047,253 - SMa1846 0.62 -1.6 1,047,253 - SMa1846 0.62 +0.6 1,047,456 - SMa1846 0.81 -0.9 1,047,682 - +0.0 1,048,308 + SMa1848 0.43 -0.2 1,048,591 + SMa1848 0.62 -2.8 1,048,592 - SMa1848 0.62 -0.4
Or see this region's nucleotide sequence