Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21124 and SM_b21125 overlap by 4 nucleotides SM_b21125 and SM_b21126 overlap by 4 nucleotides SM_b21126 and SM_b21127 are separated by 76 nucleotides SM_b21127 and SM_b21128 are separated by 6 nucleotides
SM_b21124: SM_b21124 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) <small>(from data)</small>, at 762,782 to 764,770
_b21124
SM_b21125: SM_b21125 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) <small>(from data)</small>, at 764,767 to 765,636
_b21125
SM_b21126: SM_b21126 - 3-methylglutaconyl-CoA hydratase (EC 4.2.1.18) <small>(from data)</small>, at 765,633 to 766,418
_b21126
SM_b21127: SM_b21127 - hypothetical protein, at 766,495 to 766,866
_b21127
SM_b21128: SM_b21128 - hypothetical protein, at 766,873 to 767,427
_b21128
Position (kb) 765
766
767 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 765.021 kb on + strand, within SM_b21125 at 765.021 kb on + strand, within SM_b21125 at 765.193 kb on + strand, within SM_b21125 at 765.193 kb on + strand, within SM_b21125 at 765.193 kb on + strand, within SM_b21125 at 765.194 kb on - strand, within SM_b21125 at 765.221 kb on - strand, within SM_b21125 at 765.221 kb on - strand, within SM_b21125 at 765.287 kb on - strand, within SM_b21125 at 765.402 kb on + strand, within SM_b21125 at 765.402 kb on + strand, within SM_b21125 at 765.403 kb on - strand, within SM_b21125 at 765.496 kb on + strand, within SM_b21125 at 765.526 kb on - strand, within SM_b21125 at 766.080 kb on + strand, within SM_b21126 at 766.081 kb on - strand, within SM_b21126 at 766.233 kb on + strand, within SM_b21126 at 766.233 kb on + strand, within SM_b21126 at 766.234 kb on - strand, within SM_b21126 at 766.327 kb on + strand, within SM_b21126 at 766.864 kb on + strand at 766.864 kb on + strand at 766.865 kb on - strand at 766.865 kb on - strand at 766.974 kb on + strand, within SM_b21128 at 766.974 kb on + strand, within SM_b21128 at 766.974 kb on + strand, within SM_b21128 at 766.974 kb on + strand, within SM_b21128 at 766.974 kb on + strand, within SM_b21128 at 766.975 kb on - strand, within SM_b21128 at 766.975 kb on - strand, within SM_b21128 at 766.975 kb on - strand, within SM_b21128 at 766.975 kb on - strand, within SM_b21128 at 767.081 kb on - strand, within SM_b21128 at 767.082 kb on + strand, within SM_b21128 at 767.083 kb on - strand, within SM_b21128 at 767.083 kb on - strand, within SM_b21128 at 767.284 kb on + strand, within SM_b21128 at 767.285 kb on - strand, within SM_b21128 at 767.285 kb on - strand, within SM_b21128 at 767.285 kb on - strand, within SM_b21128 at 767.299 kb on + strand, within SM_b21128 at 767.299 kb on + strand, within SM_b21128 at 767.299 kb on + strand, within SM_b21128 at 767.300 kb on - strand, within SM_b21128
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 765,021 + SM_b21125 0.29 +2.1 765,021 + SM_b21125 0.29 -0.3 765,193 + SM_b21125 0.49 +1.0 765,193 + SM_b21125 0.49 -0.9 765,193 + SM_b21125 0.49 -1.6 765,194 - SM_b21125 0.49 -1.4 765,221 - SM_b21125 0.52 -1.4 765,221 - SM_b21125 0.52 +0.0 765,287 - SM_b21125 0.60 +0.1 765,402 + SM_b21125 0.73 +0.5 765,402 + SM_b21125 0.73 +0.2 765,403 - SM_b21125 0.73 -1.2 765,496 + SM_b21125 0.84 -2.0 765,526 - SM_b21125 0.87 -1.4 766,080 + SM_b21126 0.57 +0.1 766,081 - SM_b21126 0.57 -0.2 766,233 + SM_b21126 0.76 -3.4 766,233 + SM_b21126 0.76 -1.0 766,234 - SM_b21126 0.76 -2.9 766,327 + SM_b21126 0.88 -0.1 766,864 + -0.8 766,864 + +0.1 766,865 - -0.7 766,865 - -0.0 766,974 + SM_b21128 0.18 -0.2 766,974 + SM_b21128 0.18 +0.8 766,974 + SM_b21128 0.18 -1.2 766,974 + SM_b21128 0.18 -0.4 766,974 + SM_b21128 0.18 -1.4 766,975 - SM_b21128 0.18 +0.7 766,975 - SM_b21128 0.18 +0.9 766,975 - SM_b21128 0.18 -0.1 766,975 - SM_b21128 0.18 +3.3 767,081 - SM_b21128 0.37 -1.4 767,082 + SM_b21128 0.38 +0.1 767,083 - SM_b21128 0.38 -1.2 767,083 - SM_b21128 0.38 -3.4 767,284 + SM_b21128 0.74 -0.5 767,285 - SM_b21128 0.74 +0.2 767,285 - SM_b21128 0.74 -0.4 767,285 - SM_b21128 0.74 +0.8 767,299 + SM_b21128 0.77 -1.2 767,299 + SM_b21128 0.77 -0.6 767,299 + SM_b21128 0.77 +1.6 767,300 - SM_b21128 0.77 +1.2
Or see this region's nucleotide sequence