Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20248 and SM_b20249 are separated by 151 nucleotides SM_b20249 and SM_b20250 overlap by 4 nucleotides SM_b20250 and SM_b20251 are separated by 71 nucleotides
SM_b20248: SM_b20248 - hypothetical protein, at 253,338 to 255,086
_b20248
SM_b20249: SM_b20249 - myo-inositol-1-phosphate synthase, at 255,238 to 256,341
_b20249
SM_b20250: SM_b20250 - hypothetical protein, at 256,338 to 256,937
_b20250
SM_b20251: SM_b20251 - hypothetical protein, at 257,009 to 257,971
_b20251
Position (kb) 255
256
257 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 254.687 kb on - strand, within SM_b20248 at 254.707 kb on + strand, within SM_b20248 at 254.708 kb on - strand, within SM_b20248 at 254.708 kb on - strand, within SM_b20248 at 254.708 kb on - strand, within SM_b20248 at 255.040 kb on - strand at 255.079 kb on - strand at 255.079 kb on - strand at 255.079 kb on - strand at 255.211 kb on + strand at 255.211 kb on + strand at 255.211 kb on + strand at 255.212 kb on - strand at 255.212 kb on - strand at 255.212 kb on - strand at 255.212 kb on - strand at 255.212 kb on - strand at 255.212 kb on - strand at 255.216 kb on - strand at 255.316 kb on + strand at 255.317 kb on - strand at 255.317 kb on - strand at 255.457 kb on + strand, within SM_b20249 at 255.458 kb on - strand, within SM_b20249 at 255.640 kb on + strand, within SM_b20249 at 255.641 kb on - strand, within SM_b20249 at 255.676 kb on + strand, within SM_b20249 at 255.801 kb on - strand, within SM_b20249 at 255.872 kb on + strand, within SM_b20249 at 255.873 kb on - strand, within SM_b20249 at 255.873 kb on - strand, within SM_b20249 at 255.884 kb on - strand, within SM_b20249 at 255.906 kb on + strand, within SM_b20249 at 256.067 kb on - strand, within SM_b20249 at 256.067 kb on - strand, within SM_b20249 at 256.068 kb on + strand, within SM_b20249 at 256.234 kb on + strand at 256.238 kb on + strand at 256.417 kb on + strand, within SM_b20250 at 256.494 kb on + strand, within SM_b20250 at 256.494 kb on + strand, within SM_b20250 at 256.494 kb on + strand, within SM_b20250 at 256.664 kb on + strand, within SM_b20250 at 256.664 kb on + strand, within SM_b20250 at 257.004 kb on + strand at 257.004 kb on + strand at 257.005 kb on - strand at 257.099 kb on - strand at 257.198 kb on - strand, within SM_b20251
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 254,687 - SM_b20248 0.77 +0.5 254,707 + SM_b20248 0.78 +0.5 254,708 - SM_b20248 0.78 +0.8 254,708 - SM_b20248 0.78 +0.8 254,708 - SM_b20248 0.78 -0.8 255,040 - -0.5 255,079 - -0.3 255,079 - -0.1 255,079 - -1.8 255,211 + -2.3 255,211 + -1.5 255,211 + +1.7 255,212 - -1.1 255,212 - +1.7 255,212 - -0.3 255,212 - -0.2 255,212 - +0.9 255,212 - -0.4 255,216 - -0.1 255,316 + +0.4 255,317 - -1.9 255,317 - +1.5 255,457 + SM_b20249 0.20 +1.3 255,458 - SM_b20249 0.20 -1.4 255,640 + SM_b20249 0.36 +4.9 255,641 - SM_b20249 0.37 -0.8 255,676 + SM_b20249 0.40 -0.0 255,801 - SM_b20249 0.51 +0.0 255,872 + SM_b20249 0.57 -2.7 255,873 - SM_b20249 0.58 +1.1 255,873 - SM_b20249 0.58 -0.6 255,884 - SM_b20249 0.59 -0.9 255,906 + SM_b20249 0.61 +0.3 256,067 - SM_b20249 0.75 +0.1 256,067 - SM_b20249 0.75 -0.2 256,068 + SM_b20249 0.75 -1.6 256,234 + -1.9 256,238 + +1.1 256,417 + SM_b20250 0.13 +0.9 256,494 + SM_b20250 0.26 -0.2 256,494 + SM_b20250 0.26 -0.8 256,494 + SM_b20250 0.26 +0.4 256,664 + SM_b20250 0.54 -0.7 256,664 + SM_b20250 0.54 +0.6 257,004 + -0.4 257,004 + +0.2 257,005 - -0.1 257,099 - -0.3 257,198 - SM_b20251 0.20 +0.4
Or see this region's nucleotide sequence