Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20117 and SM_b20118 are separated by 480 nucleotides SM_b20118 and SM_b20119 are separated by 22 nucleotides SM_b20119 and SM_b22004 overlap by 20 nucleotides SM_b22004 and SM_b20121 overlap by 56 nucleotides SM_b20121 and SM_b20122 are separated by 181 nucleotides
SM_b20117: SM_b20117 - sugar transferase, at 130,586 to 131,686
_b20117
SM_b20118: SM_b20118 - hypothetical protein, at 132,167 to 132,586
_b20118
SM_b20119: SM_b20119 - hypothetical protein, at 132,609 to 132,965
_b20119
SM_b22004: SM_b22004 - plasmid stabilization system protein, at 132,946 to 133,338
_b22004
SM_b20121: SM_b20121 - hypothetical protein, at 133,283 to 133,558
_b20121
SM_b20122: SM_b20122 - hypothetical protein, at 133,740 to 134,075
_b20122
Position (kb) 132
133 Strain fitness (log2 ratio) -2
-1
0
1
2 at 131.679 kb on - strand at 131.765 kb on + strand at 131.766 kb on - strand at 131.766 kb on - strand at 131.766 kb on - strand at 132.010 kb on + strand at 132.010 kb on + strand at 132.010 kb on + strand at 132.011 kb on - strand at 132.011 kb on - strand at 132.011 kb on - strand at 132.011 kb on - strand at 132.011 kb on - strand at 132.097 kb on - strand at 132.131 kb on + strand at 132.132 kb on - strand at 132.132 kb on - strand at 132.132 kb on - strand at 132.132 kb on - strand at 132.139 kb on + strand at 132.139 kb on + strand at 132.140 kb on - strand at 132.179 kb on + strand at 132.180 kb on - strand at 132.180 kb on - strand at 132.295 kb on - strand, within SM_b20118 at 132.313 kb on - strand, within SM_b20118 at 132.313 kb on - strand, within SM_b20118 at 132.332 kb on - strand, within SM_b20118 at 132.332 kb on - strand, within SM_b20118 at 132.332 kb on - strand, within SM_b20118 at 132.374 kb on - strand, within SM_b20118 at 132.392 kb on + strand, within SM_b20118 at 132.393 kb on - strand, within SM_b20118 at 132.393 kb on - strand, within SM_b20118 at 132.393 kb on - strand, within SM_b20118 at 132.443 kb on - strand, within SM_b20118 at 132.498 kb on + strand, within SM_b20118 at 132.499 kb on - strand, within SM_b20118 at 132.499 kb on - strand, within SM_b20118 at 132.499 kb on - strand, within SM_b20118 at 132.499 kb on - strand, within SM_b20118 at 132.508 kb on + strand, within SM_b20118 at 132.509 kb on - strand, within SM_b20118 at 132.611 kb on - strand at 132.848 kb on + strand, within SM_b20119 at 133.022 kb on + strand, within SM_b22004 at 133.025 kb on + strand, within SM_b22004 at 133.166 kb on + strand, within SM_b22004 at 133.167 kb on - strand, within SM_b22004 at 133.188 kb on - strand, within SM_b22004 at 133.368 kb on - strand, within SM_b20121 at 133.476 kb on + strand, within SM_b20121 at 133.609 kb on - strand at 133.610 kb on + strand at 133.654 kb on + strand at 133.654 kb on + strand at 133.654 kb on + strand at 133.689 kb on + strand at 133.689 kb on + strand at 133.702 kb on + strand at 133.723 kb on - strand at 133.723 kb on - strand at 133.830 kb on - strand, within SM_b20122 at 133.885 kb on + strand, within SM_b20122 at 133.885 kb on + strand, within SM_b20122 at 133.885 kb on + strand, within SM_b20122 at 133.886 kb on - strand, within SM_b20122 at 133.930 kb on - strand, within SM_b20122
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 131,679 - -0.4 131,765 + -0.7 131,766 - +2.3 131,766 - -0.8 131,766 - +0.4 132,010 + -0.1 132,010 + -0.5 132,010 + +1.1 132,011 - -0.3 132,011 - +0.3 132,011 - -2.3 132,011 - -0.4 132,011 - +1.1 132,097 - +0.1 132,131 + +1.6 132,132 - -1.3 132,132 - +0.5 132,132 - +0.0 132,132 - +0.2 132,139 + +0.1 132,139 + -0.2 132,140 - +0.4 132,179 + -2.5 132,180 - +0.3 132,180 - +0.7 132,295 - SM_b20118 0.30 -0.2 132,313 - SM_b20118 0.35 +0.8 132,313 - SM_b20118 0.35 -0.3 132,332 - SM_b20118 0.39 -2.7 132,332 - SM_b20118 0.39 -0.7 132,332 - SM_b20118 0.39 +2.3 132,374 - SM_b20118 0.49 +0.8 132,392 + SM_b20118 0.54 -0.0 132,393 - SM_b20118 0.54 +1.9 132,393 - SM_b20118 0.54 -0.9 132,393 - SM_b20118 0.54 -1.2 132,443 - SM_b20118 0.66 -0.4 132,498 + SM_b20118 0.79 +0.7 132,499 - SM_b20118 0.79 +1.6 132,499 - SM_b20118 0.79 +1.1 132,499 - SM_b20118 0.79 -0.7 132,499 - SM_b20118 0.79 -1.3 132,508 + SM_b20118 0.81 +0.6 132,509 - SM_b20118 0.81 -0.6 132,611 - -0.0 132,848 + SM_b20119 0.67 -0.6 133,022 + SM_b22004 0.19 -0.1 133,025 + SM_b22004 0.20 +0.2 133,166 + SM_b22004 0.56 -1.6 133,167 - SM_b22004 0.56 +0.5 133,188 - SM_b22004 0.62 +1.0 133,368 - SM_b20121 0.31 -1.0 133,476 + SM_b20121 0.70 -0.9 133,609 - -0.6 133,610 + -2.2 133,654 + -2.6 133,654 + +0.1 133,654 + +0.6 133,689 + -1.9 133,689 + -1.6 133,702 + +0.3 133,723 - -0.0 133,723 - -1.4 133,830 - SM_b20122 0.27 -0.7 133,885 + SM_b20122 0.43 -0.1 133,885 + SM_b20122 0.43 -0.8 133,885 + SM_b20122 0.43 +1.9 133,886 - SM_b20122 0.43 +0.3 133,930 - SM_b20122 0.57 +0.5
Or see this region's nucleotide sequence