Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa5022 and SMa5023 overlap by 4 nucleotides SMa5023 and SMa0444 are separated by 83 nucleotides SMa0444 and SMa0445 overlap by 4 nucleotides
SMa5022: SMa5022 - hypothetical protein, at 236,016 to 236,381
SMa5022
SMa5023: SMa5023 - hypothetical protein, at 236,378 to 236,794
SMa5023
SMa0444: SMa0444 - TRm1b transposase, at 236,878 to 237,708
SMa0444
SMa0445: SMa0445 - TRm1a transposase, at 237,705 to 238,097
SMa0445
Position (kb) 236
237 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 235.445 kb on - strand at 235.477 kb on + strand at 235.478 kb on - strand at 235.478 kb on - strand at 235.478 kb on - strand at 235.494 kb on + strand at 235.505 kb on - strand at 235.879 kb on + strand at 235.879 kb on + strand at 235.879 kb on + strand at 236.196 kb on - strand, within SMa5022 at 236.215 kb on - strand, within SMa5022 at 236.262 kb on - strand, within SMa5022 at 236.313 kb on + strand, within SMa5022 at 236.313 kb on + strand, within SMa5022 at 236.314 kb on - strand, within SMa5022 at 236.314 kb on - strand, within SMa5022 at 236.316 kb on + strand, within SMa5022 at 236.316 kb on + strand, within SMa5022 at 236.317 kb on - strand, within SMa5022 at 236.366 kb on + strand at 236.366 kb on + strand at 236.367 kb on - strand at 236.433 kb on + strand, within SMa5023 at 236.513 kb on - strand, within SMa5023 at 236.643 kb on + strand, within SMa5023 at 236.644 kb on - strand, within SMa5023 at 236.828 kb on - strand at 236.954 kb on + strand at 236.954 kb on + strand at 236.954 kb on + strand at 236.954 kb on + strand at 236.955 kb on - strand at 236.955 kb on - strand at 237.036 kb on - strand, within SMa0444
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 235,445 - -2.4 235,477 + +1.3 235,478 - +0.2 235,478 - +2.1 235,478 - +0.5 235,494 + -1.4 235,505 - -0.6 235,879 + +1.0 235,879 + -0.7 235,879 + -0.4 236,196 - SMa5022 0.49 -2.8 236,215 - SMa5022 0.54 -0.8 236,262 - SMa5022 0.67 -1.6 236,313 + SMa5022 0.81 -0.2 236,313 + SMa5022 0.81 -1.8 236,314 - SMa5022 0.81 -0.7 236,314 - SMa5022 0.81 -1.9 236,316 + SMa5022 0.82 +0.0 236,316 + SMa5022 0.82 +0.6 236,317 - SMa5022 0.82 -2.6 236,366 + -1.0 236,366 + -4.0 236,367 - -0.4 236,433 + SMa5023 0.13 -1.6 236,513 - SMa5023 0.32 -0.9 236,643 + SMa5023 0.64 -1.0 236,644 - SMa5023 0.64 -1.9 236,828 - +0.0 236,954 + -2.1 236,954 + -1.0 236,954 + -1.9 236,954 + +0.7 236,955 - +0.1 236,955 - -1.9 237,036 - SMa0444 0.19 +1.1
Or see this region's nucleotide sequence