Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0824 and SMa0825 are separated by 230 nucleotides
SMa0824: SMa0824 - hypothetical protein, at 452,858 to 453,325
SMa0824
SMa0825: SMa0825 - nitrogenase reductase, at 453,556 to 454,449
SMa0825
Position (kb) 452
453
454 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 451.860 kb on - strand at 451.880 kb on + strand at 451.881 kb on - strand at 451.962 kb on + strand at 452.230 kb on + strand at 452.301 kb on + strand at 452.302 kb on - strand at 452.302 kb on - strand at 452.302 kb on - strand at 452.345 kb on + strand at 452.346 kb on - strand at 452.361 kb on + strand at 452.362 kb on - strand at 452.415 kb on + strand at 452.461 kb on - strand at 452.479 kb on + strand at 452.480 kb on - strand at 452.484 kb on - strand at 452.714 kb on + strand at 452.763 kb on - strand at 452.796 kb on + strand at 452.837 kb on + strand at 453.153 kb on + strand, within SMa0824 at 453.153 kb on + strand, within SMa0824 at 453.153 kb on + strand, within SMa0824 at 453.153 kb on + strand, within SMa0824 at 453.154 kb on - strand, within SMa0824 at 453.159 kb on + strand, within SMa0824 at 453.159 kb on + strand, within SMa0824 at 453.160 kb on - strand, within SMa0824 at 453.418 kb on + strand at 453.418 kb on + strand at 453.419 kb on - strand at 453.531 kb on + strand at 453.536 kb on + strand at 453.537 kb on - strand at 453.583 kb on + strand at 453.584 kb on - strand at 453.624 kb on + strand at 453.731 kb on + strand, within SMa0825 at 453.867 kb on + strand, within SMa0825 at 453.904 kb on + strand, within SMa0825 at 453.928 kb on + strand, within SMa0825 at 453.947 kb on + strand, within SMa0825 at 453.948 kb on - strand, within SMa0825 at 453.973 kb on - strand, within SMa0825 at 454.034 kb on - strand, within SMa0825 at 454.059 kb on + strand, within SMa0825 at 454.261 kb on - strand, within SMa0825 at 454.261 kb on - strand, within SMa0825
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 451,860 - -0.6 451,880 + -1.7 451,881 - -0.8 451,962 + +0.6 452,230 + -2.0 452,301 + -3.3 452,302 - -1.6 452,302 - +0.5 452,302 - -0.2 452,345 + +2.3 452,346 - -0.0 452,361 + -1.9 452,362 - -3.0 452,415 + +0.6 452,461 - -3.4 452,479 + -1.0 452,480 - -1.0 452,484 - -0.8 452,714 + -0.0 452,763 - +0.8 452,796 + +2.8 452,837 + +0.6 453,153 + SMa0824 0.63 -0.5 453,153 + SMa0824 0.63 -3.5 453,153 + SMa0824 0.63 +1.3 453,153 + SMa0824 0.63 -0.9 453,154 - SMa0824 0.63 +2.3 453,159 + SMa0824 0.64 -0.5 453,159 + SMa0824 0.64 -0.3 453,160 - SMa0824 0.65 -0.1 453,418 + -0.2 453,418 + +1.1 453,419 - -1.6 453,531 + -0.5 453,536 + +0.0 453,537 - -0.2 453,583 + -0.4 453,584 - -0.9 453,624 + -0.4 453,731 + SMa0825 0.20 -0.1 453,867 + SMa0825 0.35 -1.0 453,904 + SMa0825 0.39 -1.0 453,928 + SMa0825 0.42 +1.4 453,947 + SMa0825 0.44 -1.6 453,948 - SMa0825 0.44 +1.0 453,973 - SMa0825 0.47 -2.7 454,034 - SMa0825 0.53 -0.8 454,059 + SMa0825 0.56 +0.8 454,261 - SMa0825 0.79 -0.7 454,261 - SMa0825 0.79 -0.0
Or see this region's nucleotide sequence