Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1024 and Pf6N2E2_1025 are separated by 12 nucleotides Pf6N2E2_1025 and Pf6N2E2_1026 are separated by 99 nucleotides
Pf6N2E2_1024 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 1,197,062 to 1,198,414
_1024
Pf6N2E2_1025 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,198,427 to 1,199,380
_1025
Pf6N2E2_1026 - Rhodanese-related sulfurtransferase, at 1,199,480 to 1,201,063
_1026
Position (kb)
1198
1199
1200 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1197.675 kb on + strand, within Pf6N2E2_1024 at 1197.897 kb on + strand, within Pf6N2E2_1024 at 1197.898 kb on - strand, within Pf6N2E2_1024 at 1198.116 kb on + strand, within Pf6N2E2_1024 at 1198.207 kb on - strand, within Pf6N2E2_1024 at 1198.299 kb on + strand at 1198.527 kb on + strand, within Pf6N2E2_1025 at 1198.528 kb on - strand, within Pf6N2E2_1025 at 1198.528 kb on - strand, within Pf6N2E2_1025 at 1198.736 kb on - strand, within Pf6N2E2_1025 at 1198.767 kb on + strand, within Pf6N2E2_1025 at 1198.767 kb on + strand, within Pf6N2E2_1025 at 1198.974 kb on + strand, within Pf6N2E2_1025 at 1199.071 kb on + strand, within Pf6N2E2_1025 at 1199.072 kb on - strand, within Pf6N2E2_1025 at 1199.139 kb on + strand, within Pf6N2E2_1025 at 1199.139 kb on + strand, within Pf6N2E2_1025 at 1199.139 kb on + strand, within Pf6N2E2_1025 at 1199.139 kb on + strand, within Pf6N2E2_1025 at 1199.140 kb on - strand, within Pf6N2E2_1025 at 1199.140 kb on - strand, within Pf6N2E2_1025 at 1199.446 kb on + strand at 1199.446 kb on + strand at 1199.535 kb on + strand at 1199.535 kb on + strand at 1199.536 kb on - strand at 1199.536 kb on - strand at 1199.550 kb on + strand at 1199.589 kb on + strand at 1199.590 kb on - strand at 1199.601 kb on + strand at 1199.772 kb on + strand, within Pf6N2E2_1026 at 1199.772 kb on + strand, within Pf6N2E2_1026 at 1199.773 kb on - strand, within Pf6N2E2_1026 at 1199.773 kb on - strand, within Pf6N2E2_1026 at 1199.859 kb on + strand, within Pf6N2E2_1026 at 1199.860 kb on - strand, within Pf6N2E2_1026 at 1199.860 kb on - strand, within Pf6N2E2_1026 at 1199.860 kb on - strand, within Pf6N2E2_1026 at 1199.860 kb on - strand, within Pf6N2E2_1026 at 1200.021 kb on + strand, within Pf6N2E2_1026 at 1200.021 kb on + strand, within Pf6N2E2_1026 at 1200.021 kb on + strand, within Pf6N2E2_1026 at 1200.022 kb on - strand, within Pf6N2E2_1026 at 1200.022 kb on - strand, within Pf6N2E2_1026 at 1200.022 kb on - strand, within Pf6N2E2_1026 at 1200.219 kb on + strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026 at 1200.220 kb on - strand, within Pf6N2E2_1026
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 1,197,675 + Pf6N2E2_1024 0.45 +1.4 1,197,897 + Pf6N2E2_1024 0.62 +0.4 1,197,898 - Pf6N2E2_1024 0.62 -0.4 1,198,116 + Pf6N2E2_1024 0.78 -0.2 1,198,207 - Pf6N2E2_1024 0.85 -0.8 1,198,299 + +1.3 1,198,527 + Pf6N2E2_1025 0.10 -1.4 1,198,528 - Pf6N2E2_1025 0.11 +0.4 1,198,528 - Pf6N2E2_1025 0.11 +1.0 1,198,736 - Pf6N2E2_1025 0.32 -1.7 1,198,767 + Pf6N2E2_1025 0.36 +0.1 1,198,767 + Pf6N2E2_1025 0.36 -0.9 1,198,974 + Pf6N2E2_1025 0.57 +0.4 1,199,071 + Pf6N2E2_1025 0.68 -0.6 1,199,072 - Pf6N2E2_1025 0.68 -0.1 1,199,139 + Pf6N2E2_1025 0.75 +1.4 1,199,139 + Pf6N2E2_1025 0.75 +1.4 1,199,139 + Pf6N2E2_1025 0.75 -1.6 1,199,139 + Pf6N2E2_1025 0.75 -2.2 1,199,140 - Pf6N2E2_1025 0.75 -0.2 1,199,140 - Pf6N2E2_1025 0.75 -0.6 1,199,446 + +1.0 1,199,446 + -0.2 1,199,535 + -0.2 1,199,535 + -0.9 1,199,536 - +0.4 1,199,536 - -1.2 1,199,550 + +0.7 1,199,589 + +0.1 1,199,590 - +0.0 1,199,601 + +0.4 1,199,772 + Pf6N2E2_1026 0.18 -0.4 1,199,772 + Pf6N2E2_1026 0.18 +0.8 1,199,773 - Pf6N2E2_1026 0.18 -0.3 1,199,773 - Pf6N2E2_1026 0.18 +3.0 1,199,859 + Pf6N2E2_1026 0.24 +0.4 1,199,860 - Pf6N2E2_1026 0.24 +1.0 1,199,860 - Pf6N2E2_1026 0.24 +0.8 1,199,860 - Pf6N2E2_1026 0.24 +1.0 1,199,860 - Pf6N2E2_1026 0.24 -1.3 1,200,021 + Pf6N2E2_1026 0.34 +0.5 1,200,021 + Pf6N2E2_1026 0.34 +0.9 1,200,021 + Pf6N2E2_1026 0.34 +0.4 1,200,022 - Pf6N2E2_1026 0.34 -0.7 1,200,022 - Pf6N2E2_1026 0.34 -0.6 1,200,022 - Pf6N2E2_1026 0.34 +0.4 1,200,219 + Pf6N2E2_1026 0.47 +0.4 1,200,220 - Pf6N2E2_1026 0.47 -1.2 1,200,220 - Pf6N2E2_1026 0.47 -1.7 1,200,220 - Pf6N2E2_1026 0.47 -0.4 1,200,220 - Pf6N2E2_1026 0.47 +2.1 1,200,220 - Pf6N2E2_1026 0.47 +1.4 1,200,220 - Pf6N2E2_1026 0.47 +1.0 1,200,220 - Pf6N2E2_1026 0.47 -1.9
Or see this region's nucleotide sequence