Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_97 and Pf6N2E2_98 overlap by 4 nucleotides Pf6N2E2_98 and Pf6N2E2_99 are separated by 53 nucleotides Pf6N2E2_99 and Pf6N2E2_100 are separated by 298 nucleotides
Pf6N2E2_97 - putataive branched-chain amino acid transport protein AzlC, at 142,645 to 143,355
_97
Pf6N2E2_98 - putative transmembrane protein, at 143,352 to 143,660
_98
Pf6N2E2_99 - hypothetical protein, at 143,714 to 143,854
_99
Pf6N2E2_100 - membrane protein, putative, at 144,153 to 145,364
_100
Position (kb)
143
144 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 142.788 kb on + strand, within Pf6N2E2_97 at 142.788 kb on + strand, within Pf6N2E2_97 at 142.788 kb on + strand, within Pf6N2E2_97 at 142.788 kb on + strand, within Pf6N2E2_97 at 142.789 kb on - strand, within Pf6N2E2_97 at 142.800 kb on - strand, within Pf6N2E2_97 at 143.230 kb on - strand, within Pf6N2E2_97 at 143.392 kb on + strand, within Pf6N2E2_98 at 143.393 kb on - strand, within Pf6N2E2_98 at 143.629 kb on + strand, within Pf6N2E2_98 at 143.630 kb on - strand at 143.757 kb on - strand, within Pf6N2E2_99 at 143.757 kb on - strand, within Pf6N2E2_99 at 143.757 kb on - strand, within Pf6N2E2_99 at 143.768 kb on - strand, within Pf6N2E2_99 at 143.870 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.924 kb on - strand at 143.924 kb on - strand at 143.954 kb on - strand at 143.954 kb on - strand at 143.976 kb on - strand at 144.117 kb on + strand at 144.117 kb on + strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.128 kb on + strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.172 kb on + strand at 144.172 kb on + strand at 144.172 kb on + strand at 144.173 kb on - strand at 144.173 kb on - strand at 144.196 kb on + strand at 144.196 kb on + strand at 144.196 kb on + strand at 144.197 kb on - strand at 144.197 kb on - strand at 144.197 kb on - strand at 144.276 kb on - strand, within Pf6N2E2_100 at 144.300 kb on + strand, within Pf6N2E2_100 at 144.403 kb on + strand, within Pf6N2E2_100 at 144.404 kb on - strand, within Pf6N2E2_100 at 144.511 kb on + strand, within Pf6N2E2_100 at 144.511 kb on + strand, within Pf6N2E2_100 at 144.512 kb on - strand, within Pf6N2E2_100 at 144.512 kb on - strand, within Pf6N2E2_100 at 144.600 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.631 kb on + strand, within Pf6N2E2_100 at 144.827 kb on - strand, within Pf6N2E2_100
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 142,788 + Pf6N2E2_97 0.20 +0.6 142,788 + Pf6N2E2_97 0.20 -0.5 142,788 + Pf6N2E2_97 0.20 -0.5 142,788 + Pf6N2E2_97 0.20 -1.3 142,789 - Pf6N2E2_97 0.20 +1.8 142,800 - Pf6N2E2_97 0.22 -0.8 143,230 - Pf6N2E2_97 0.82 -0.6 143,392 + Pf6N2E2_98 0.13 +1.5 143,393 - Pf6N2E2_98 0.13 +2.8 143,629 + Pf6N2E2_98 0.90 -0.3 143,630 - +0.2 143,757 - Pf6N2E2_99 0.30 -2.0 143,757 - Pf6N2E2_99 0.30 -0.0 143,757 - Pf6N2E2_99 0.30 -1.5 143,768 - Pf6N2E2_99 0.38 -0.3 143,870 + -0.2 143,923 + +2.4 143,923 + +0.6 143,923 + +1.1 143,923 + -0.2 143,923 + -0.0 143,923 + -0.8 143,924 - +0.6 143,924 - -0.3 143,954 - +0.9 143,954 - +0.1 143,976 - -0.6 144,117 + -0.5 144,117 + -1.2 144,118 - +0.2 144,118 - +1.8 144,118 - -1.0 144,118 - +0.8 144,128 + +0.2 144,129 - +0.8 144,129 - -0.4 144,129 - -0.2 144,129 - -0.6 144,132 - +1.3 144,132 - +0.8 144,132 - -0.8 144,132 - +0.0 144,132 - -0.2 144,172 + +0.8 144,172 + -0.2 144,172 + -1.2 144,173 - +0.5 144,173 - -1.4 144,196 + -0.2 144,196 + +1.1 144,196 + +0.0 144,197 - +1.5 144,197 - -0.5 144,197 - +0.3 144,276 - Pf6N2E2_100 0.10 +0.1 144,300 + Pf6N2E2_100 0.12 +0.1 144,403 + Pf6N2E2_100 0.21 +0.4 144,404 - Pf6N2E2_100 0.21 +1.8 144,511 + Pf6N2E2_100 0.30 -0.8 144,511 + Pf6N2E2_100 0.30 -2.0 144,512 - Pf6N2E2_100 0.30 +0.8 144,512 - Pf6N2E2_100 0.30 -0.4 144,600 + Pf6N2E2_100 0.37 -0.5 144,610 + Pf6N2E2_100 0.38 -1.4 144,610 + Pf6N2E2_100 0.38 +0.8 144,610 + Pf6N2E2_100 0.38 -0.7 144,631 + Pf6N2E2_100 0.39 +0.8 144,827 - Pf6N2E2_100 0.56 -1.2
Or see this region's nucleotide sequence