Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_945 and Pf6N2E2_946 are separated by 172 nucleotides Pf6N2E2_946 and Pf6N2E2_947 are separated by 140 nucleotides Pf6N2E2_947 and Pf6N2E2_948 are separated by 11 nucleotides
Pf6N2E2_945 - Transcriptional regulator, TetR family, at 1,113,032 to 1,113,610
_945
Pf6N2E2_946 - Transcriptional regulator, LysR family, at 1,113,783 to 1,114,061
_946
Pf6N2E2_947 - Multidrug efflux membrane fusion protein MexE, at 1,114,202 to 1,115,434
_947
Pf6N2E2_948 - Multidrug efflux transporter MexF, at 1,115,446 to 1,118,637
_948
Position (kb)
1114
1115
1116 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1113.329 kb on - strand, within Pf6N2E2_945 at 1113.437 kb on + strand, within Pf6N2E2_945 at 1113.468 kb on + strand, within Pf6N2E2_945 at 1113.792 kb on - strand at 1113.854 kb on + strand, within Pf6N2E2_946 at 1113.854 kb on + strand, within Pf6N2E2_946 at 1113.855 kb on - strand, within Pf6N2E2_946 at 1113.855 kb on - strand, within Pf6N2E2_946 at 1113.855 kb on - strand, within Pf6N2E2_946 at 1113.881 kb on - strand, within Pf6N2E2_946 at 1114.034 kb on + strand at 1114.059 kb on + strand at 1114.060 kb on - strand at 1114.060 kb on - strand at 1114.115 kb on + strand at 1114.197 kb on + strand at 1114.197 kb on + strand at 1114.197 kb on + strand at 1114.197 kb on + strand at 1114.197 kb on + strand at 1114.201 kb on + strand at 1114.201 kb on + strand at 1114.201 kb on + strand at 1114.202 kb on - strand at 1114.202 kb on - strand at 1114.254 kb on + strand at 1114.254 kb on + strand at 1114.775 kb on - strand, within Pf6N2E2_947 at 1114.836 kb on - strand, within Pf6N2E2_947 at 1114.836 kb on - strand, within Pf6N2E2_947 at 1114.836 kb on - strand, within Pf6N2E2_947 at 1114.916 kb on + strand, within Pf6N2E2_947 at 1114.916 kb on + strand, within Pf6N2E2_947 at 1114.917 kb on - strand, within Pf6N2E2_947 at 1114.917 kb on - strand, within Pf6N2E2_947 at 1114.917 kb on - strand, within Pf6N2E2_947 at 1114.917 kb on - strand, within Pf6N2E2_947 at 1115.042 kb on + strand, within Pf6N2E2_947 at 1115.042 kb on + strand, within Pf6N2E2_947 at 1115.217 kb on + strand, within Pf6N2E2_947 at 1115.410 kb on - strand at 1115.410 kb on - strand at 1115.596 kb on + strand at 1115.597 kb on - strand at 1115.680 kb on + strand at 1115.680 kb on + strand at 1115.680 kb on + strand at 1115.680 kb on + strand at 1115.680 kb on + strand at 1115.680 kb on + strand at 1115.680 kb on + strand at 1115.681 kb on - strand at 1115.681 kb on - strand at 1115.681 kb on - strand at 1115.681 kb on - strand at 1115.681 kb on - strand at 1115.681 kb on - strand at 1115.897 kb on - strand, within Pf6N2E2_948 at 1115.897 kb on - strand, within Pf6N2E2_948 at 1115.908 kb on + strand, within Pf6N2E2_948 at 1115.909 kb on - strand, within Pf6N2E2_948 at 1115.909 kb on - strand, within Pf6N2E2_948 at 1115.920 kb on + strand, within Pf6N2E2_948 at 1115.999 kb on - strand, within Pf6N2E2_948 at 1116.138 kb on + strand, within Pf6N2E2_948 at 1116.138 kb on + strand, within Pf6N2E2_948 at 1116.138 kb on + strand, within Pf6N2E2_948 at 1116.138 kb on + strand, within Pf6N2E2_948 at 1116.278 kb on - strand, within Pf6N2E2_948 at 1116.422 kb on - strand, within Pf6N2E2_948 at 1116.433 kb on + strand, within Pf6N2E2_948 at 1116.434 kb on - strand, within Pf6N2E2_948
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,113,329 - Pf6N2E2_945 0.51 +0.6 1,113,437 + Pf6N2E2_945 0.70 -1.0 1,113,468 + Pf6N2E2_945 0.75 +0.6 1,113,792 - -0.4 1,113,854 + Pf6N2E2_946 0.25 -1.4 1,113,854 + Pf6N2E2_946 0.25 -0.0 1,113,855 - Pf6N2E2_946 0.26 -0.4 1,113,855 - Pf6N2E2_946 0.26 +0.6 1,113,855 - Pf6N2E2_946 0.26 -1.8 1,113,881 - Pf6N2E2_946 0.35 -0.4 1,114,034 + +0.6 1,114,059 + -0.4 1,114,060 - +0.3 1,114,060 - +0.2 1,114,115 + +0.6 1,114,197 + -0.2 1,114,197 + +0.1 1,114,197 + +1.6 1,114,197 + +0.7 1,114,197 + +1.6 1,114,201 + +0.7 1,114,201 + +0.9 1,114,201 + -1.2 1,114,202 - +2.9 1,114,202 - +0.6 1,114,254 + -1.4 1,114,254 + -1.2 1,114,775 - Pf6N2E2_947 0.46 -1.0 1,114,836 - Pf6N2E2_947 0.51 -2.2 1,114,836 - Pf6N2E2_947 0.51 +0.7 1,114,836 - Pf6N2E2_947 0.51 -2.4 1,114,916 + Pf6N2E2_947 0.58 -1.8 1,114,916 + Pf6N2E2_947 0.58 +0.6 1,114,917 - Pf6N2E2_947 0.58 -2.0 1,114,917 - Pf6N2E2_947 0.58 -0.4 1,114,917 - Pf6N2E2_947 0.58 +0.2 1,114,917 - Pf6N2E2_947 0.58 -0.8 1,115,042 + Pf6N2E2_947 0.68 +0.2 1,115,042 + Pf6N2E2_947 0.68 -0.4 1,115,217 + Pf6N2E2_947 0.82 -0.9 1,115,410 - -0.4 1,115,410 - +0.6 1,115,596 + +0.6 1,115,597 - -1.0 1,115,680 + +1.2 1,115,680 + -0.0 1,115,680 + -0.4 1,115,680 + -1.0 1,115,680 + -0.6 1,115,680 + +2.6 1,115,680 + -0.4 1,115,681 - +0.6 1,115,681 - -0.0 1,115,681 - +1.2 1,115,681 - +2.2 1,115,681 - -1.0 1,115,681 - -1.2 1,115,897 - Pf6N2E2_948 0.14 +1.5 1,115,897 - Pf6N2E2_948 0.14 -1.8 1,115,908 + Pf6N2E2_948 0.14 +0.2 1,115,909 - Pf6N2E2_948 0.15 +0.2 1,115,909 - Pf6N2E2_948 0.15 -0.0 1,115,920 + Pf6N2E2_948 0.15 -0.0 1,115,999 - Pf6N2E2_948 0.17 -0.8 1,116,138 + Pf6N2E2_948 0.22 -0.4 1,116,138 + Pf6N2E2_948 0.22 +0.6 1,116,138 + Pf6N2E2_948 0.22 +0.4 1,116,138 + Pf6N2E2_948 0.22 +0.6 1,116,278 - Pf6N2E2_948 0.26 -0.4 1,116,422 - Pf6N2E2_948 0.31 +0.7 1,116,433 + Pf6N2E2_948 0.31 +0.6 1,116,434 - Pf6N2E2_948 0.31 +2.9
Or see this region's nucleotide sequence