Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_896 and Pf6N2E2_897 are separated by 21 nucleotides Pf6N2E2_897 and Pf6N2E2_898 are separated by 24 nucleotides Pf6N2E2_898 and Pf6N2E2_899 are separated by 449 nucleotides
Pf6N2E2_896 - Regulatory protein, LysR:LysR, substrate-binding, at 1,049,160 to 1,050,077
_896
Pf6N2E2_897 - Maleate cis-trans isomerase (EC 5.2.1.1), at 1,050,099 to 1,050,851
_897
Pf6N2E2_898 - putative major facilitator superfamily (MFS) transporter, at 1,050,876 to 1,052,195
_898
Pf6N2E2_899 - hypothetical protein, at 1,052,645 to 1,052,776
_899
Position (kb)
1050
1051
1052
1053 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1050.052 kb on - strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.077 kb on + strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.078 kb on - strand at 1050.080 kb on + strand at 1050.080 kb on + strand at 1050.080 kb on + strand at 1050.081 kb on - strand at 1050.081 kb on - strand at 1050.081 kb on - strand at 1050.081 kb on - strand at 1050.081 kb on - strand at 1050.088 kb on + strand at 1050.089 kb on - strand at 1050.089 kb on - strand at 1050.135 kb on + strand at 1050.366 kb on + strand, within Pf6N2E2_897 at 1050.442 kb on + strand, within Pf6N2E2_897 at 1050.442 kb on + strand, within Pf6N2E2_897 at 1050.442 kb on + strand, within Pf6N2E2_897 at 1050.443 kb on - strand, within Pf6N2E2_897 at 1050.443 kb on - strand, within Pf6N2E2_897 at 1050.443 kb on - strand, within Pf6N2E2_897 at 1050.469 kb on + strand, within Pf6N2E2_897 at 1050.619 kb on + strand, within Pf6N2E2_897 at 1050.620 kb on - strand, within Pf6N2E2_897 at 1050.620 kb on - strand, within Pf6N2E2_897 at 1050.620 kb on - strand, within Pf6N2E2_897 at 1050.766 kb on + strand, within Pf6N2E2_897 at 1050.946 kb on + strand at 1050.947 kb on - strand at 1051.324 kb on + strand, within Pf6N2E2_898 at 1051.325 kb on - strand, within Pf6N2E2_898 at 1051.405 kb on + strand, within Pf6N2E2_898 at 1051.405 kb on + strand, within Pf6N2E2_898 at 1051.405 kb on + strand, within Pf6N2E2_898 at 1051.441 kb on + strand, within Pf6N2E2_898 at 1051.441 kb on + strand, within Pf6N2E2_898 at 1051.441 kb on + strand, within Pf6N2E2_898 at 1051.441 kb on + strand, within Pf6N2E2_898 at 1051.441 kb on + strand, within Pf6N2E2_898 at 1051.441 kb on + strand, within Pf6N2E2_898 at 1051.442 kb on - strand, within Pf6N2E2_898 at 1051.442 kb on - strand, within Pf6N2E2_898 at 1051.442 kb on - strand, within Pf6N2E2_898 at 1051.442 kb on - strand, within Pf6N2E2_898 at 1051.442 kb on - strand, within Pf6N2E2_898 at 1051.442 kb on - strand, within Pf6N2E2_898 at 1051.442 kb on - strand, within Pf6N2E2_898 at 1051.484 kb on + strand, within Pf6N2E2_898 at 1051.490 kb on - strand, within Pf6N2E2_898 at 1051.490 kb on - strand, within Pf6N2E2_898 at 1051.536 kb on - strand, within Pf6N2E2_898 at 1051.609 kb on + strand, within Pf6N2E2_898 at 1051.610 kb on - strand, within Pf6N2E2_898 at 1051.610 kb on - strand, within Pf6N2E2_898 at 1051.627 kb on + strand, within Pf6N2E2_898 at 1051.627 kb on + strand, within Pf6N2E2_898 at 1051.628 kb on - strand, within Pf6N2E2_898 at 1051.628 kb on - strand, within Pf6N2E2_898 at 1051.628 kb on - strand, within Pf6N2E2_898 at 1051.628 kb on - strand, within Pf6N2E2_898 at 1051.888 kb on + strand, within Pf6N2E2_898 at 1051.889 kb on - strand, within Pf6N2E2_898 at 1052.046 kb on + strand, within Pf6N2E2_898 at 1052.047 kb on - strand, within Pf6N2E2_898 at 1052.116 kb on + strand at 1052.117 kb on - strand at 1052.222 kb on + strand at 1052.284 kb on + strand at 1052.284 kb on + strand at 1052.284 kb on + strand at 1052.284 kb on + strand at 1052.285 kb on - strand at 1052.306 kb on + strand at 1052.306 kb on + strand at 1052.307 kb on - strand at 1052.307 kb on - strand at 1052.307 kb on - strand at 1052.307 kb on - strand at 1052.307 kb on - strand at 1052.307 kb on - strand at 1052.308 kb on + strand at 1052.309 kb on - strand at 1052.395 kb on - strand at 1052.395 kb on - strand at 1052.418 kb on - strand at 1052.505 kb on + strand at 1052.506 kb on - strand at 1052.531 kb on + strand at 1052.532 kb on - strand at 1052.559 kb on - strand at 1052.581 kb on - strand at 1052.630 kb on + strand at 1052.630 kb on + strand at 1052.630 kb on + strand at 1052.630 kb on + strand at 1052.630 kb on + strand at 1052.631 kb on - strand at 1052.631 kb on - strand at 1052.631 kb on - strand at 1052.639 kb on - strand at 1052.639 kb on - strand at 1052.657 kb on + strand at 1052.657 kb on + strand at 1052.658 kb on - strand at 1052.725 kb on + strand, within Pf6N2E2_899 at 1052.790 kb on - strand at 1052.814 kb on - strand at 1052.853 kb on + strand at 1052.854 kb on - strand at 1052.854 kb on - strand at 1052.882 kb on + strand at 1052.969 kb on + strand at 1052.969 kb on + strand at 1052.970 kb on - strand at 1053.061 kb on + strand at 1053.062 kb on - strand at 1053.062 kb on - strand at 1053.080 kb on + strand at 1053.080 kb on + strand at 1053.081 kb on - strand at 1053.081 kb on - strand at 1053.083 kb on + strand at 1053.083 kb on + strand at 1053.083 kb on + strand at 1053.083 kb on + strand at 1053.083 kb on + strand at 1053.097 kb on + strand at 1053.097 kb on + strand at 1053.097 kb on + strand at 1053.097 kb on + strand at 1053.097 kb on + strand at 1053.098 kb on - strand at 1053.115 kb on + strand at 1053.115 kb on + strand at 1053.115 kb on + strand at 1053.115 kb on + strand at 1053.115 kb on + strand at 1053.116 kb on - strand at 1053.122 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,050,052 - -0.2 1,050,077 + +0.0 1,050,077 + +0.7 1,050,077 + +1.2 1,050,077 + +0.9 1,050,077 + -0.1 1,050,077 + -0.9 1,050,077 + +0.0 1,050,077 + -1.7 1,050,077 + +0.1 1,050,078 - -0.4 1,050,078 - -0.4 1,050,078 - +0.8 1,050,078 - +1.6 1,050,078 - -0.4 1,050,078 - +0.6 1,050,078 - -1.3 1,050,078 - +0.3 1,050,078 - -0.6 1,050,078 - -0.5 1,050,080 + +0.2 1,050,080 + -2.4 1,050,080 + -0.1 1,050,081 - -0.4 1,050,081 - +0.2 1,050,081 - -0.1 1,050,081 - +0.3 1,050,081 - +0.8 1,050,088 + +0.3 1,050,089 - -1.7 1,050,089 - +0.2 1,050,135 + +0.0 1,050,366 + Pf6N2E2_897 0.35 -0.3 1,050,442 + Pf6N2E2_897 0.46 +0.0 1,050,442 + Pf6N2E2_897 0.46 -1.4 1,050,442 + Pf6N2E2_897 0.46 -1.0 1,050,443 - Pf6N2E2_897 0.46 +0.6 1,050,443 - Pf6N2E2_897 0.46 +0.6 1,050,443 - Pf6N2E2_897 0.46 +0.2 1,050,469 + Pf6N2E2_897 0.49 -0.8 1,050,619 + Pf6N2E2_897 0.69 -0.4 1,050,620 - Pf6N2E2_897 0.69 -0.4 1,050,620 - Pf6N2E2_897 0.69 +0.6 1,050,620 - Pf6N2E2_897 0.69 -1.0 1,050,766 + Pf6N2E2_897 0.89 -1.0 1,050,946 + -1.7 1,050,947 - +0.1 1,051,324 + Pf6N2E2_898 0.34 +0.1 1,051,325 - Pf6N2E2_898 0.34 +0.2 1,051,405 + Pf6N2E2_898 0.40 -0.1 1,051,405 + Pf6N2E2_898 0.40 -1.4 1,051,405 + Pf6N2E2_898 0.40 +0.6 1,051,441 + Pf6N2E2_898 0.43 +0.6 1,051,441 + Pf6N2E2_898 0.43 +0.0 1,051,441 + Pf6N2E2_898 0.43 +0.6 1,051,441 + Pf6N2E2_898 0.43 +0.9 1,051,441 + Pf6N2E2_898 0.43 -0.2 1,051,441 + Pf6N2E2_898 0.43 +1.6 1,051,442 - Pf6N2E2_898 0.43 -1.4 1,051,442 - Pf6N2E2_898 0.43 +0.3 1,051,442 - Pf6N2E2_898 0.43 +0.0 1,051,442 - Pf6N2E2_898 0.43 -0.8 1,051,442 - Pf6N2E2_898 0.43 +0.6 1,051,442 - Pf6N2E2_898 0.43 -0.2 1,051,442 - Pf6N2E2_898 0.43 +1.2 1,051,484 + Pf6N2E2_898 0.46 +1.7 1,051,490 - Pf6N2E2_898 0.47 +1.0 1,051,490 - Pf6N2E2_898 0.47 -0.4 1,051,536 - Pf6N2E2_898 0.50 -0.4 1,051,609 + Pf6N2E2_898 0.56 -0.4 1,051,610 - Pf6N2E2_898 0.56 -2.4 1,051,610 - Pf6N2E2_898 0.56 +0.4 1,051,627 + Pf6N2E2_898 0.57 +1.3 1,051,627 + Pf6N2E2_898 0.57 +0.6 1,051,628 - Pf6N2E2_898 0.57 +2.6 1,051,628 - Pf6N2E2_898 0.57 +1.6 1,051,628 - Pf6N2E2_898 0.57 -0.2 1,051,628 - Pf6N2E2_898 0.57 -1.0 1,051,888 + Pf6N2E2_898 0.77 +1.2 1,051,889 - Pf6N2E2_898 0.77 +0.6 1,052,046 + Pf6N2E2_898 0.89 +1.6 1,052,047 - Pf6N2E2_898 0.89 +2.6 1,052,116 + -0.6 1,052,117 - +0.6 1,052,222 + -0.5 1,052,284 + -0.4 1,052,284 + +0.8 1,052,284 + +0.6 1,052,284 + -0.7 1,052,285 - -0.1 1,052,306 + +0.6 1,052,306 + -0.1 1,052,307 - +0.1 1,052,307 - -0.4 1,052,307 - +0.1 1,052,307 - -0.1 1,052,307 - +0.6 1,052,307 - +0.6 1,052,308 + -1.4 1,052,309 - +0.4 1,052,395 - -0.7 1,052,395 - +0.9 1,052,418 - +0.0 1,052,505 + +0.6 1,052,506 - -0.4 1,052,531 + +0.6 1,052,532 - +0.0 1,052,559 - +0.0 1,052,581 - +0.6 1,052,630 + +1.2 1,052,630 + +0.0 1,052,630 + +0.6 1,052,630 + +0.6 1,052,630 + +0.6 1,052,631 - +1.2 1,052,631 - -1.0 1,052,631 - -0.6 1,052,639 - -0.4 1,052,639 - +2.2 1,052,657 + -0.4 1,052,657 + +1.6 1,052,658 - -0.3 1,052,725 + Pf6N2E2_899 0.61 +0.6 1,052,790 - +0.2 1,052,814 - -0.5 1,052,853 + -0.2 1,052,854 - -0.4 1,052,854 - -0.3 1,052,882 + -0.4 1,052,969 + -0.5 1,052,969 + +2.0 1,052,970 - -1.4 1,053,061 + +0.3 1,053,062 - +1.6 1,053,062 - +0.6 1,053,080 + +1.2 1,053,080 + +0.4 1,053,081 - +0.0 1,053,081 - +0.3 1,053,083 + -1.7 1,053,083 + -0.4 1,053,083 + +1.6 1,053,083 + +0.1 1,053,083 + -0.4 1,053,097 + -0.7 1,053,097 + +1.0 1,053,097 + -0.2 1,053,097 + -0.0 1,053,097 + +0.6 1,053,098 - -0.4 1,053,115 + -0.4 1,053,115 + -1.1 1,053,115 + +2.2 1,053,115 + -0.3 1,053,115 + +1.3 1,053,116 - +0.6 1,053,122 + +1.6
Or see this region's nucleotide sequence