Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_720

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_718 and Pf6N2E2_719 are separated by 88 nucleotidesPf6N2E2_719 and Pf6N2E2_720 overlap by 4 nucleotidesPf6N2E2_720 and Pf6N2E2_721 are separated by 81 nucleotides Pf6N2E2_718 - Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5), at 822,406 to 823,053 _718 Pf6N2E2_719 - Molybdenum cofactor biosynthesis protein MoaB, at 823,142 to 823,696 _719 Pf6N2E2_720 - Molybdopterin biosynthesis protein MoeA, at 823,693 to 824,928 _720 Pf6N2E2_721 - FIG139552: Putative protease, at 825,010 to 826,005 _721 Position (kb) 823 824 825Strain fitness (log2 ratio) -1 0 1at 822.732 kb on - strand, within Pf6N2E2_718at 822.732 kb on - strand, within Pf6N2E2_718at 822.732 kb on - strand, within Pf6N2E2_718at 822.782 kb on - strand, within Pf6N2E2_718at 823.067 kb on + strandat 823.067 kb on + strandat 823.067 kb on + strandat 823.068 kb on - strandat 823.068 kb on - strandat 823.084 kb on - strandat 823.193 kb on - strandat 823.303 kb on + strand, within Pf6N2E2_719at 823.308 kb on - strand, within Pf6N2E2_719at 823.886 kb on - strand, within Pf6N2E2_720at 824.228 kb on - strand, within Pf6N2E2_720at 824.228 kb on - strand, within Pf6N2E2_720at 824.308 kb on + strand, within Pf6N2E2_720at 824.308 kb on + strand, within Pf6N2E2_720at 824.309 kb on - strand, within Pf6N2E2_720at 824.309 kb on - strand, within Pf6N2E2_720at 824.325 kb on + strand, within Pf6N2E2_720at 824.688 kb on - strand, within Pf6N2E2_720at 824.743 kb on + strand, within Pf6N2E2_720at 824.743 kb on + strand, within Pf6N2E2_720at 824.747 kb on + strand, within Pf6N2E2_720at 824.747 kb on + strand, within Pf6N2E2_720at 824.899 kb on + strandat 825.082 kb on + strandat 825.082 kb on + strandat 825.082 kb on + strandat 825.082 kb on + strandat 825.082 kb on + strandat 825.082 kb on + strandat 825.082 kb on + strandat 825.083 kb on - strandat 825.083 kb on - strandat 825.193 kb on + strand, within Pf6N2E2_721at 825.193 kb on + strand, within Pf6N2E2_721at 825.193 kb on + strand, within Pf6N2E2_721at 825.207 kb on + strand, within Pf6N2E2_721at 825.208 kb on - strand, within Pf6N2E2_721at 825.208 kb on - strand, within Pf6N2E2_721at 825.357 kb on - strand, within Pf6N2E2_721at 825.550 kb on + strand, within Pf6N2E2_721at 825.550 kb on + strand, within Pf6N2E2_721at 825.551 kb on - strand, within Pf6N2E2_721at 825.551 kb on - strand, within Pf6N2E2_721at 825.551 kb on - strand, within Pf6N2E2_721at 825.551 kb on - strand, within Pf6N2E2_721at 825.668 kb on - strand, within Pf6N2E2_721at 825.847 kb on + strand, within Pf6N2E2_721at 825.847 kb on + strand, within Pf6N2E2_721at 825.847 kb on + strand, within Pf6N2E2_721at 825.848 kb on - strand, within Pf6N2E2_721at 825.848 kb on - strand, within Pf6N2E2_721

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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822,732 - Pf6N2E2_718 0.50 +0.2
822,732 - Pf6N2E2_718 0.50 +0.7
822,732 - Pf6N2E2_718 0.50 -1.0
822,782 - Pf6N2E2_718 0.58 +0.6
823,067 + +0.6
823,067 + +0.6
823,067 + -0.1
823,068 - -1.0
823,068 - -0.4
823,084 - -1.0
823,193 - +0.6
823,303 + Pf6N2E2_719 0.29 +1.1
823,308 - Pf6N2E2_719 0.30 -0.8
823,886 - Pf6N2E2_720 0.16 -0.4
824,228 - Pf6N2E2_720 0.43 -1.0
824,228 - Pf6N2E2_720 0.43 +1.6
824,308 + Pf6N2E2_720 0.50 -0.9
824,308 + Pf6N2E2_720 0.50 -0.4
824,309 - Pf6N2E2_720 0.50 +0.3
824,309 - Pf6N2E2_720 0.50 +0.3
824,325 + Pf6N2E2_720 0.51 -0.9
824,688 - Pf6N2E2_720 0.81 -1.0
824,743 + Pf6N2E2_720 0.85 +0.4
824,743 + Pf6N2E2_720 0.85 +0.2
824,747 + Pf6N2E2_720 0.85 +0.2
824,747 + Pf6N2E2_720 0.85 +0.3
824,899 + -0.4
825,082 + -0.1
825,082 + +0.6
825,082 + +0.1
825,082 + +1.4
825,082 + -0.1
825,082 + -0.0
825,082 + -0.8
825,083 - +0.8
825,083 - -1.4
825,193 + Pf6N2E2_721 0.18 -1.0
825,193 + Pf6N2E2_721 0.18 -0.2
825,193 + Pf6N2E2_721 0.18 -0.6
825,207 + Pf6N2E2_721 0.20 +1.1
825,208 - Pf6N2E2_721 0.20 -1.7
825,208 - Pf6N2E2_721 0.20 -1.7
825,357 - Pf6N2E2_721 0.35 +0.7
825,550 + Pf6N2E2_721 0.54 -0.1
825,550 + Pf6N2E2_721 0.54 -0.4
825,551 - Pf6N2E2_721 0.54 +0.8
825,551 - Pf6N2E2_721 0.54 -1.4
825,551 - Pf6N2E2_721 0.54 +0.8
825,551 - Pf6N2E2_721 0.54 +0.4
825,668 - Pf6N2E2_721 0.66 -1.5
825,847 + Pf6N2E2_721 0.84 -0.0
825,847 + Pf6N2E2_721 0.84 +0.2
825,847 + Pf6N2E2_721 0.84 -0.2
825,848 - Pf6N2E2_721 0.84 +0.3
825,848 - Pf6N2E2_721 0.84 +0.0

Or see this region's nucleotide sequence