Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_637 and Pf6N2E2_638 are separated by 195 nucleotides Pf6N2E2_638 and Pf6N2E2_639 are separated by 180 nucleotides Pf6N2E2_639 and Pf6N2E2_640 are separated by 205 nucleotides Pf6N2E2_640 and Pf6N2E2_641 overlap by 34 nucleotides
Pf6N2E2_637 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 728,851 to 730,284
_637
Pf6N2E2_638 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 730,480 to 730,920
_638
Pf6N2E2_639 - FIG01285020: hypothetical protein, at 731,101 to 731,442
_639
Pf6N2E2_640 - Outer membrane TonB-dependent transducer VreA of trans-envelope signaling system, at 731,648 to 732,280
_640
Pf6N2E2_641 - Extracytoplasmic function (ECF) sigma factor VreI, at 732,247 to 732,822
_641
Position (kb) 731
732 Strain fitness (log2 ratio) -2
-1
0
1
2 at 730.101 kb on - strand, within Pf6N2E2_637 at 730.101 kb on - strand, within Pf6N2E2_637 at 730.115 kb on + strand, within Pf6N2E2_637 at 730.116 kb on - strand, within Pf6N2E2_637 at 730.232 kb on + strand at 730.233 kb on - strand at 730.233 kb on - strand at 730.233 kb on - strand at 730.358 kb on + strand at 730.359 kb on - strand at 730.426 kb on + strand at 730.498 kb on + strand at 730.498 kb on + strand at 730.501 kb on + strand at 730.501 kb on + strand at 730.502 kb on - strand at 730.502 kb on - strand at 730.502 kb on - strand at 730.502 kb on - strand at 730.502 kb on - strand at 730.801 kb on + strand, within Pf6N2E2_638 at 730.801 kb on + strand, within Pf6N2E2_638 at 730.802 kb on - strand, within Pf6N2E2_638 at 730.802 kb on - strand, within Pf6N2E2_638 at 731.029 kb on - strand at 731.103 kb on - strand at 731.169 kb on + strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.495 kb on + strand at 731.528 kb on + strand at 731.528 kb on + strand at 731.529 kb on - strand at 731.540 kb on - strand at 731.540 kb on - strand at 731.572 kb on + strand at 731.573 kb on - strand at 731.573 kb on - strand at 731.803 kb on + strand, within Pf6N2E2_640 at 731.803 kb on + strand, within Pf6N2E2_640 at 731.804 kb on - strand, within Pf6N2E2_640 at 731.882 kb on + strand, within Pf6N2E2_640 at 731.975 kb on + strand, within Pf6N2E2_640 at 731.975 kb on + strand, within Pf6N2E2_640 at 731.976 kb on - strand, within Pf6N2E2_640 at 732.228 kb on + strand at 732.229 kb on - strand at 732.253 kb on - strand at 732.325 kb on + strand, within Pf6N2E2_641 at 732.326 kb on - strand, within Pf6N2E2_641 at 732.326 kb on - strand, within Pf6N2E2_641 at 732.401 kb on - strand, within Pf6N2E2_641 at 732.401 kb on - strand, within Pf6N2E2_641
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 730,101 - Pf6N2E2_637 0.87 -2.5 730,101 - Pf6N2E2_637 0.87 +1.3 730,115 + Pf6N2E2_637 0.88 +0.5 730,116 - Pf6N2E2_637 0.88 +1.6 730,232 + +0.6 730,233 - -1.4 730,233 - +2.4 730,233 - -1.7 730,358 + -0.1 730,359 - -2.2 730,426 + -0.4 730,498 + +1.6 730,498 + +1.6 730,501 + -0.0 730,501 + -1.7 730,502 - +1.0 730,502 - +1.2 730,502 - -1.0 730,502 - -0.2 730,502 - +0.6 730,801 + Pf6N2E2_638 0.73 +0.4 730,801 + Pf6N2E2_638 0.73 -1.5 730,802 - Pf6N2E2_638 0.73 -1.8 730,802 - Pf6N2E2_638 0.73 -0.1 731,029 - +0.8 731,103 - +0.2 731,169 + Pf6N2E2_639 0.20 -0.2 731,170 - Pf6N2E2_639 0.20 -1.0 731,170 - Pf6N2E2_639 0.20 -0.7 731,170 - Pf6N2E2_639 0.20 -0.8 731,170 - Pf6N2E2_639 0.20 -0.2 731,495 + +0.0 731,528 + +0.4 731,528 + +0.3 731,529 - -0.4 731,540 - -0.7 731,540 - -0.2 731,572 + +0.6 731,573 - +0.2 731,573 - +0.2 731,803 + Pf6N2E2_640 0.24 +0.0 731,803 + Pf6N2E2_640 0.24 +2.6 731,804 - Pf6N2E2_640 0.25 +0.6 731,882 + Pf6N2E2_640 0.37 +0.8 731,975 + Pf6N2E2_640 0.52 -0.3 731,975 + Pf6N2E2_640 0.52 +1.0 731,976 - Pf6N2E2_640 0.52 +0.6 732,228 + -0.4 732,229 - +0.0 732,253 - +0.2 732,325 + Pf6N2E2_641 0.14 +1.6 732,326 - Pf6N2E2_641 0.14 -0.4 732,326 - Pf6N2E2_641 0.14 +1.6 732,401 - Pf6N2E2_641 0.27 +0.6 732,401 - Pf6N2E2_641 0.27 -0.4
Or see this region's nucleotide sequence