Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5682 and Pf6N2E2_5683 are separated by 164 nucleotides Pf6N2E2_5683 and Pf6N2E2_6161 are separated by 598 nucleotides
Pf6N2E2_5682 - Transcriptional regulator, AraC family, at 6,495,268 to 6,496,080
_5682
Pf6N2E2_5683 - Alginate biosynthesis transcriptional activator, at 6,496,245 to 6,496,499
_5683
Pf6N2E2_6161 - tRNA-Arg-CCT, at 6,497,098 to 6,497,171
_6161
Position (kb)
6496
6497 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6495.290 kb on + strand at 6495.290 kb on + strand at 6495.290 kb on + strand at 6495.290 kb on + strand at 6495.290 kb on + strand at 6495.290 kb on + strand at 6495.290 kb on + strand at 6495.290 kb on + strand at 6495.291 kb on - strand at 6495.291 kb on - strand at 6495.291 kb on - strand at 6495.291 kb on - strand at 6495.291 kb on - strand at 6495.336 kb on + strand at 6495.337 kb on - strand at 6495.337 kb on - strand at 6495.438 kb on - strand, within Pf6N2E2_5682 at 6495.438 kb on - strand, within Pf6N2E2_5682 at 6495.438 kb on - strand, within Pf6N2E2_5682 at 6495.698 kb on + strand, within Pf6N2E2_5682 at 6495.698 kb on + strand, within Pf6N2E2_5682 at 6495.698 kb on + strand, within Pf6N2E2_5682 at 6495.699 kb on - strand, within Pf6N2E2_5682 at 6495.787 kb on - strand, within Pf6N2E2_5682 at 6495.824 kb on + strand, within Pf6N2E2_5682 at 6495.824 kb on + strand, within Pf6N2E2_5682 at 6495.825 kb on - strand, within Pf6N2E2_5682 at 6495.896 kb on - strand, within Pf6N2E2_5682 at 6495.896 kb on - strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.919 kb on + strand, within Pf6N2E2_5682 at 6495.919 kb on + strand, within Pf6N2E2_5682 at 6495.920 kb on - strand, within Pf6N2E2_5682 at 6495.920 kb on - strand, within Pf6N2E2_5682 at 6495.990 kb on + strand, within Pf6N2E2_5682 at 6495.990 kb on + strand, within Pf6N2E2_5682 at 6495.991 kb on - strand, within Pf6N2E2_5682 at 6495.991 kb on - strand, within Pf6N2E2_5682 at 6496.118 kb on + strand at 6496.119 kb on - strand at 6496.163 kb on + strand at 6496.164 kb on - strand at 6496.232 kb on + strand at 6496.416 kb on - strand, within Pf6N2E2_5683 at 6496.516 kb on - strand at 6496.571 kb on + strand at 6496.571 kb on + strand at 6496.571 kb on + strand at 6496.571 kb on + strand at 6496.572 kb on - strand at 6496.572 kb on - strand at 6496.572 kb on - strand at 6496.572 kb on - strand at 6496.572 kb on - strand at 6496.572 kb on - strand at 6496.572 kb on - strand at 6496.572 kb on - strand at 6496.573 kb on + strand at 6496.574 kb on - strand at 6496.574 kb on - strand at 6496.574 kb on - strand at 6496.574 kb on - strand at 6496.574 kb on - strand at 6496.574 kb on - strand at 6496.574 kb on - strand at 6496.574 kb on - strand at 6496.574 kb on - strand at 6496.574 kb on - strand at 6496.577 kb on + strand at 6496.628 kb on + strand at 6496.629 kb on - strand at 6496.828 kb on + strand at 6496.828 kb on + strand at 6496.828 kb on + strand at 6496.828 kb on + strand at 6496.828 kb on + strand at 6496.829 kb on - strand at 6496.891 kb on - strand at 6496.917 kb on - strand at 6496.919 kb on + strand at 6496.983 kb on + strand at 6496.983 kb on + strand at 6496.984 kb on - strand at 6496.993 kb on + strand at 6496.993 kb on + strand at 6496.993 kb on + strand at 6496.993 kb on + strand at 6496.994 kb on - strand at 6496.994 kb on - strand at 6496.994 kb on - strand at 6496.994 kb on - strand at 6496.996 kb on + strand at 6496.996 kb on + strand at 6496.996 kb on + strand at 6496.996 kb on + strand at 6496.996 kb on + strand at 6496.996 kb on + strand at 6496.996 kb on + strand at 6496.997 kb on - strand at 6496.997 kb on - strand at 6497.005 kb on - strand at 6497.005 kb on - strand at 6497.005 kb on - strand at 6497.011 kb on - strand at 6497.011 kb on - strand at 6497.175 kb on + strand at 6497.178 kb on + strand at 6497.179 kb on - strand at 6497.238 kb on + strand at 6497.238 kb on + strand at 6497.239 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 6,495,290 + -0.0 6,495,290 + +0.2 6,495,290 + +0.5 6,495,290 + +0.4 6,495,290 + +1.0 6,495,290 + +2.0 6,495,290 + -1.5 6,495,290 + +0.4 6,495,291 - -0.5 6,495,291 - +1.0 6,495,291 - +1.6 6,495,291 - +0.3 6,495,291 - -0.1 6,495,336 + +0.3 6,495,337 - +1.0 6,495,337 - -1.5 6,495,438 - Pf6N2E2_5682 0.21 +0.2 6,495,438 - Pf6N2E2_5682 0.21 +0.0 6,495,438 - Pf6N2E2_5682 0.21 -0.5 6,495,698 + Pf6N2E2_5682 0.53 +0.1 6,495,698 + Pf6N2E2_5682 0.53 -0.5 6,495,698 + Pf6N2E2_5682 0.53 -0.4 6,495,699 - Pf6N2E2_5682 0.53 +1.9 6,495,787 - Pf6N2E2_5682 0.64 +0.8 6,495,824 + Pf6N2E2_5682 0.68 -0.3 6,495,824 + Pf6N2E2_5682 0.68 +1.0 6,495,825 - Pf6N2E2_5682 0.69 +1.6 6,495,896 - Pf6N2E2_5682 0.77 -0.5 6,495,896 - Pf6N2E2_5682 0.77 +0.4 6,495,897 + Pf6N2E2_5682 0.77 -1.0 6,495,897 + Pf6N2E2_5682 0.77 -1.0 6,495,897 + Pf6N2E2_5682 0.77 -0.3 6,495,897 + Pf6N2E2_5682 0.77 -0.2 6,495,897 + Pf6N2E2_5682 0.77 +2.4 6,495,897 + Pf6N2E2_5682 0.77 -0.2 6,495,897 + Pf6N2E2_5682 0.77 +1.0 6,495,897 + Pf6N2E2_5682 0.77 -0.3 6,495,897 + Pf6N2E2_5682 0.77 -0.2 6,495,898 - Pf6N2E2_5682 0.77 +0.1 6,495,898 - Pf6N2E2_5682 0.77 -1.5 6,495,898 - Pf6N2E2_5682 0.77 -0.1 6,495,898 - Pf6N2E2_5682 0.77 +0.9 6,495,898 - Pf6N2E2_5682 0.77 -0.2 6,495,898 - Pf6N2E2_5682 0.77 -0.4 6,495,898 - Pf6N2E2_5682 0.77 -0.3 6,495,898 - Pf6N2E2_5682 0.77 -0.4 6,495,898 - Pf6N2E2_5682 0.77 +0.8 6,495,898 - Pf6N2E2_5682 0.77 +0.0 6,495,919 + Pf6N2E2_5682 0.80 -0.1 6,495,919 + Pf6N2E2_5682 0.80 +0.3 6,495,920 - Pf6N2E2_5682 0.80 -0.5 6,495,920 - Pf6N2E2_5682 0.80 +1.1 6,495,990 + Pf6N2E2_5682 0.89 -0.5 6,495,990 + Pf6N2E2_5682 0.89 -2.3 6,495,991 - Pf6N2E2_5682 0.89 +1.7 6,495,991 - Pf6N2E2_5682 0.89 -1.1 6,496,118 + +1.0 6,496,119 - +0.5 6,496,163 + +0.0 6,496,164 - +0.9 6,496,232 + +1.0 6,496,416 - Pf6N2E2_5683 0.67 -0.4 6,496,516 - -1.0 6,496,571 + +1.0 6,496,571 + +0.5 6,496,571 + -1.5 6,496,571 + +0.0 6,496,572 - +0.0 6,496,572 - +0.5 6,496,572 - -1.0 6,496,572 - -1.2 6,496,572 - +0.5 6,496,572 - +0.9 6,496,572 - +1.0 6,496,572 - -1.3 6,496,573 + +0.3 6,496,574 - -0.8 6,496,574 - +0.3 6,496,574 - +0.7 6,496,574 - +0.5 6,496,574 - -0.0 6,496,574 - +1.0 6,496,574 - +0.3 6,496,574 - -0.2 6,496,574 - +1.8 6,496,574 - -0.1 6,496,577 + -1.8 6,496,628 + +1.0 6,496,629 - +1.0 6,496,828 + +0.5 6,496,828 + +0.7 6,496,828 + -1.0 6,496,828 + +0.7 6,496,828 + +0.0 6,496,829 - +0.2 6,496,891 - -0.7 6,496,917 - +0.2 6,496,919 + -0.1 6,496,983 + +1.0 6,496,983 + +1.0 6,496,984 - -1.1 6,496,993 + +1.0 6,496,993 + +0.3 6,496,993 + +0.7 6,496,993 + +0.0 6,496,994 - -0.2 6,496,994 - +0.0 6,496,994 - -1.5 6,496,994 - -0.5 6,496,996 + -0.7 6,496,996 + -1.3 6,496,996 + -0.3 6,496,996 + -0.8 6,496,996 + -0.5 6,496,996 + +0.8 6,496,996 + +0.5 6,496,997 - +0.0 6,496,997 - +1.0 6,497,005 - +0.9 6,497,005 - +1.3 6,497,005 - +0.7 6,497,011 - -0.0 6,497,011 - -0.1 6,497,175 + -0.5 6,497,178 + +0.6 6,497,179 - +1.3 6,497,238 + -0.6 6,497,238 + -0.1 6,497,239 - +1.6
Or see this region's nucleotide sequence