Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5502 and Pf6N2E2_5503 overlap by 14 nucleotides Pf6N2E2_5503 and Pf6N2E2_5504 overlap by 4 nucleotides Pf6N2E2_5504 and Pf6N2E2_5505 are separated by 12 nucleotides Pf6N2E2_5505 and Pf6N2E2_5506 are separated by 119 nucleotides
Pf6N2E2_5502 - 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases, at 6,319,949 to 6,321,493
_5502
Pf6N2E2_5503 - FIG01248689: hypothetical protein, at 6,321,480 to 6,322,454
_5503
Pf6N2E2_5504 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 6,322,451 to 6,323,176
_5504
Pf6N2E2_5505 - hypothetical protein, at 6,323,189 to 6,323,314
_5505
Pf6N2E2_5506 - Exodeoxyribonuclease I (EC 3.1.11.1), at 6,323,434 to 6,324,870
_5506
Position (kb)
6322
6323
6324 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6321.520 kb on + strand at 6321.520 kb on + strand at 6321.520 kb on + strand at 6321.521 kb on - strand at 6321.521 kb on - strand at 6321.559 kb on + strand at 6321.560 kb on - strand at 6321.560 kb on - strand at 6321.651 kb on + strand, within Pf6N2E2_5503 at 6321.652 kb on - strand, within Pf6N2E2_5503 at 6321.946 kb on + strand, within Pf6N2E2_5503 at 6321.946 kb on + strand, within Pf6N2E2_5503 at 6321.946 kb on + strand, within Pf6N2E2_5503 at 6321.946 kb on + strand, within Pf6N2E2_5503 at 6321.946 kb on + strand, within Pf6N2E2_5503 at 6321.946 kb on + strand, within Pf6N2E2_5503 at 6321.946 kb on + strand, within Pf6N2E2_5503 at 6321.947 kb on - strand, within Pf6N2E2_5503 at 6321.947 kb on - strand, within Pf6N2E2_5503 at 6321.947 kb on - strand, within Pf6N2E2_5503 at 6321.947 kb on - strand, within Pf6N2E2_5503 at 6321.947 kb on - strand, within Pf6N2E2_5503 at 6321.947 kb on - strand, within Pf6N2E2_5503 at 6321.947 kb on - strand, within Pf6N2E2_5503 at 6321.947 kb on - strand, within Pf6N2E2_5503 at 6321.947 kb on - strand, within Pf6N2E2_5503 at 6321.947 kb on - strand, within Pf6N2E2_5503 at 6321.947 kb on - strand, within Pf6N2E2_5503 at 6321.947 kb on - strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.021 kb on + strand, within Pf6N2E2_5503 at 6322.022 kb on - strand, within Pf6N2E2_5503 at 6322.022 kb on - strand, within Pf6N2E2_5503 at 6322.022 kb on - strand, within Pf6N2E2_5503 at 6322.022 kb on - strand, within Pf6N2E2_5503 at 6322.022 kb on - strand, within Pf6N2E2_5503 at 6322.022 kb on - strand, within Pf6N2E2_5503 at 6322.022 kb on - strand, within Pf6N2E2_5503 at 6322.022 kb on - strand, within Pf6N2E2_5503 at 6322.022 kb on - strand, within Pf6N2E2_5503 at 6322.022 kb on - strand, within Pf6N2E2_5503 at 6322.022 kb on - strand, within Pf6N2E2_5503 at 6322.022 kb on - strand, within Pf6N2E2_5503 at 6322.022 kb on - strand, within Pf6N2E2_5503 at 6322.022 kb on - strand, within Pf6N2E2_5503 at 6322.067 kb on - strand, within Pf6N2E2_5503 at 6322.067 kb on - strand, within Pf6N2E2_5503 at 6322.067 kb on - strand, within Pf6N2E2_5503 at 6322.087 kb on + strand, within Pf6N2E2_5503 at 6322.088 kb on - strand, within Pf6N2E2_5503 at 6322.088 kb on - strand, within Pf6N2E2_5503 at 6322.232 kb on - strand, within Pf6N2E2_5503 at 6322.336 kb on + strand, within Pf6N2E2_5503 at 6322.337 kb on - strand, within Pf6N2E2_5503 at 6322.337 kb on - strand, within Pf6N2E2_5503 at 6322.337 kb on - strand, within Pf6N2E2_5503 at 6322.337 kb on - strand, within Pf6N2E2_5503 at 6322.923 kb on + strand, within Pf6N2E2_5504 at 6322.923 kb on + strand, within Pf6N2E2_5504 at 6322.923 kb on + strand, within Pf6N2E2_5504 at 6322.923 kb on + strand, within Pf6N2E2_5504 at 6322.924 kb on - strand, within Pf6N2E2_5504 at 6322.924 kb on - strand, within Pf6N2E2_5504 at 6322.924 kb on - strand, within Pf6N2E2_5504 at 6322.924 kb on - strand, within Pf6N2E2_5504 at 6322.924 kb on - strand, within Pf6N2E2_5504 at 6322.924 kb on - strand, within Pf6N2E2_5504 at 6323.025 kb on - strand, within Pf6N2E2_5504 at 6323.226 kb on + strand, within Pf6N2E2_5505 at 6323.226 kb on + strand, within Pf6N2E2_5505 at 6323.251 kb on + strand, within Pf6N2E2_5505 at 6323.310 kb on + strand at 6323.310 kb on + strand at 6323.310 kb on + strand at 6323.311 kb on - strand at 6323.311 kb on - strand at 6323.401 kb on - strand at 6323.401 kb on - strand at 6323.458 kb on + strand at 6323.459 kb on - strand at 6323.560 kb on + strand at 6323.560 kb on + strand at 6323.845 kb on + strand, within Pf6N2E2_5506 at 6323.895 kb on - strand, within Pf6N2E2_5506 at 6324.096 kb on + strand, within Pf6N2E2_5506 at 6324.096 kb on + strand, within Pf6N2E2_5506 at 6324.097 kb on - strand, within Pf6N2E2_5506 at 6324.127 kb on + strand, within Pf6N2E2_5506 at 6324.128 kb on - strand, within Pf6N2E2_5506 at 6324.128 kb on - strand, within Pf6N2E2_5506
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 6,321,520 + +0.0 6,321,520 + +0.1 6,321,520 + -0.1 6,321,521 - -0.2 6,321,521 - +0.8 6,321,559 + -0.6 6,321,560 - +0.5 6,321,560 - +0.2 6,321,651 + Pf6N2E2_5503 0.18 +0.3 6,321,652 - Pf6N2E2_5503 0.18 +0.8 6,321,946 + Pf6N2E2_5503 0.48 -0.2 6,321,946 + Pf6N2E2_5503 0.48 +0.2 6,321,946 + Pf6N2E2_5503 0.48 +0.7 6,321,946 + Pf6N2E2_5503 0.48 -0.2 6,321,946 + Pf6N2E2_5503 0.48 +0.6 6,321,946 + Pf6N2E2_5503 0.48 +0.2 6,321,946 + Pf6N2E2_5503 0.48 +0.1 6,321,947 - Pf6N2E2_5503 0.48 -0.5 6,321,947 - Pf6N2E2_5503 0.48 +0.6 6,321,947 - Pf6N2E2_5503 0.48 -0.8 6,321,947 - Pf6N2E2_5503 0.48 +0.9 6,321,947 - Pf6N2E2_5503 0.48 +0.5 6,321,947 - Pf6N2E2_5503 0.48 +0.6 6,321,947 - Pf6N2E2_5503 0.48 +0.8 6,321,947 - Pf6N2E2_5503 0.48 +1.1 6,321,947 - Pf6N2E2_5503 0.48 +1.1 6,321,947 - Pf6N2E2_5503 0.48 -0.5 6,321,947 - Pf6N2E2_5503 0.48 +0.1 6,321,947 - Pf6N2E2_5503 0.48 -1.0 6,322,021 + Pf6N2E2_5503 0.55 -0.3 6,322,021 + Pf6N2E2_5503 0.55 -1.1 6,322,021 + Pf6N2E2_5503 0.55 +0.6 6,322,021 + Pf6N2E2_5503 0.55 -0.2 6,322,021 + Pf6N2E2_5503 0.55 +0.9 6,322,021 + Pf6N2E2_5503 0.55 -2.1 6,322,021 + Pf6N2E2_5503 0.55 +0.4 6,322,021 + Pf6N2E2_5503 0.55 -0.2 6,322,021 + Pf6N2E2_5503 0.55 -0.3 6,322,021 + Pf6N2E2_5503 0.55 -0.1 6,322,021 + Pf6N2E2_5503 0.55 +0.9 6,322,021 + Pf6N2E2_5503 0.55 +0.6 6,322,021 + Pf6N2E2_5503 0.55 +0.9 6,322,021 + Pf6N2E2_5503 0.55 -0.4 6,322,021 + Pf6N2E2_5503 0.55 -0.3 6,322,021 + Pf6N2E2_5503 0.55 +0.8 6,322,021 + Pf6N2E2_5503 0.55 +0.7 6,322,022 - Pf6N2E2_5503 0.56 -0.3 6,322,022 - Pf6N2E2_5503 0.56 -0.7 6,322,022 - Pf6N2E2_5503 0.56 +0.1 6,322,022 - Pf6N2E2_5503 0.56 +0.4 6,322,022 - Pf6N2E2_5503 0.56 +1.5 6,322,022 - Pf6N2E2_5503 0.56 +0.1 6,322,022 - Pf6N2E2_5503 0.56 +0.1 6,322,022 - Pf6N2E2_5503 0.56 -1.5 6,322,022 - Pf6N2E2_5503 0.56 -0.9 6,322,022 - Pf6N2E2_5503 0.56 +1.2 6,322,022 - Pf6N2E2_5503 0.56 +0.5 6,322,022 - Pf6N2E2_5503 0.56 +0.4 6,322,022 - Pf6N2E2_5503 0.56 -0.2 6,322,022 - Pf6N2E2_5503 0.56 -0.2 6,322,067 - Pf6N2E2_5503 0.60 +2.1 6,322,067 - Pf6N2E2_5503 0.60 +0.3 6,322,067 - Pf6N2E2_5503 0.60 +0.5 6,322,087 + Pf6N2E2_5503 0.62 +0.5 6,322,088 - Pf6N2E2_5503 0.62 -0.2 6,322,088 - Pf6N2E2_5503 0.62 +0.3 6,322,232 - Pf6N2E2_5503 0.77 +1.4 6,322,336 + Pf6N2E2_5503 0.88 +0.1 6,322,337 - Pf6N2E2_5503 0.88 +0.4 6,322,337 - Pf6N2E2_5503 0.88 +0.1 6,322,337 - Pf6N2E2_5503 0.88 +2.7 6,322,337 - Pf6N2E2_5503 0.88 +0.3 6,322,923 + Pf6N2E2_5504 0.65 -0.1 6,322,923 + Pf6N2E2_5504 0.65 +1.1 6,322,923 + Pf6N2E2_5504 0.65 +0.7 6,322,923 + Pf6N2E2_5504 0.65 +0.8 6,322,924 - Pf6N2E2_5504 0.65 +0.2 6,322,924 - Pf6N2E2_5504 0.65 +1.2 6,322,924 - Pf6N2E2_5504 0.65 -0.5 6,322,924 - Pf6N2E2_5504 0.65 +0.1 6,322,924 - Pf6N2E2_5504 0.65 +1.2 6,322,924 - Pf6N2E2_5504 0.65 +0.4 6,323,025 - Pf6N2E2_5504 0.79 +0.9 6,323,226 + Pf6N2E2_5505 0.29 +0.1 6,323,226 + Pf6N2E2_5505 0.29 +0.2 6,323,251 + Pf6N2E2_5505 0.49 +0.4 6,323,310 + -0.1 6,323,310 + +1.1 6,323,310 + -0.5 6,323,311 - +0.6 6,323,311 - -0.3 6,323,401 - +0.9 6,323,401 - +1.1 6,323,458 + +0.1 6,323,459 - -0.1 6,323,560 + -1.5 6,323,560 + -0.1 6,323,845 + Pf6N2E2_5506 0.29 -0.5 6,323,895 - Pf6N2E2_5506 0.32 +0.9 6,324,096 + Pf6N2E2_5506 0.46 -0.3 6,324,096 + Pf6N2E2_5506 0.46 -1.7 6,324,097 - Pf6N2E2_5506 0.46 -0.2 6,324,127 + Pf6N2E2_5506 0.48 -0.4 6,324,128 - Pf6N2E2_5506 0.48 +0.8 6,324,128 - Pf6N2E2_5506 0.48 +0.1
Or see this region's nucleotide sequence