Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4445 and Pf6N2E2_4446 are separated by 303 nucleotides Pf6N2E2_4446 and Pf6N2E2_4447 are separated by 3 nucleotides Pf6N2E2_4447 and Pf6N2E2_4448 are separated by 106 nucleotides Pf6N2E2_4448 and Pf6N2E2_4449 are separated by 54 nucleotides
Pf6N2E2_4445 - TPR domain protein, at 5,175,964 to 5,177,364
_4445
Pf6N2E2_4446 - hypothetical protein, at 5,177,668 to 5,177,781
_4446
Pf6N2E2_4447 - Cytochrome c family protein, at 5,177,785 to 5,179,572
_4447
Pf6N2E2_4448 - probable P23 protein, at 5,179,679 to 5,180,134
_4448
Pf6N2E2_4449 - Sigma cross-reacting protein 27A, at 5,180,189 to 5,180,857
_4449
Position (kb) 5177
5178
5179
5180 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5176.805 kb on + strand, within Pf6N2E2_4445 at 5176.806 kb on - strand, within Pf6N2E2_4445 at 5176.806 kb on - strand, within Pf6N2E2_4445 at 5177.013 kb on + strand, within Pf6N2E2_4445 at 5177.014 kb on - strand, within Pf6N2E2_4445 at 5177.048 kb on + strand, within Pf6N2E2_4445 at 5177.049 kb on - strand, within Pf6N2E2_4445 at 5177.049 kb on - strand, within Pf6N2E2_4445 at 5177.049 kb on - strand, within Pf6N2E2_4445 at 5177.171 kb on + strand, within Pf6N2E2_4445 at 5177.171 kb on + strand, within Pf6N2E2_4445 at 5177.172 kb on - strand, within Pf6N2E2_4445 at 5177.172 kb on - strand, within Pf6N2E2_4445 at 5177.174 kb on + strand, within Pf6N2E2_4445 at 5177.174 kb on + strand, within Pf6N2E2_4445 at 5177.174 kb on + strand, within Pf6N2E2_4445 at 5177.174 kb on + strand, within Pf6N2E2_4445 at 5177.174 kb on + strand, within Pf6N2E2_4445 at 5177.174 kb on + strand, within Pf6N2E2_4445 at 5177.175 kb on - strand, within Pf6N2E2_4445 at 5177.175 kb on - strand, within Pf6N2E2_4445 at 5177.175 kb on - strand, within Pf6N2E2_4445 at 5177.175 kb on - strand, within Pf6N2E2_4445 at 5177.175 kb on - strand, within Pf6N2E2_4445 at 5177.282 kb on + strand at 5177.282 kb on + strand at 5177.430 kb on + strand at 5177.431 kb on - strand at 5177.464 kb on + strand at 5177.465 kb on - strand at 5177.498 kb on + strand at 5177.498 kb on + strand at 5177.498 kb on + strand at 5177.499 kb on - strand at 5177.511 kb on + strand at 5177.511 kb on + strand at 5177.511 kb on + strand at 5177.511 kb on + strand at 5177.511 kb on + strand at 5177.512 kb on - strand at 5177.515 kb on + strand at 5177.516 kb on - strand at 5177.709 kb on - strand, within Pf6N2E2_4446 at 5177.783 kb on + strand at 5177.783 kb on + strand at 5177.783 kb on + strand at 5177.791 kb on + strand at 5177.791 kb on + strand at 5177.792 kb on - strand at 5178.034 kb on + strand, within Pf6N2E2_4447 at 5178.034 kb on + strand, within Pf6N2E2_4447 at 5178.034 kb on + strand, within Pf6N2E2_4447 at 5178.034 kb on + strand, within Pf6N2E2_4447 at 5178.034 kb on + strand, within Pf6N2E2_4447 at 5178.034 kb on + strand, within Pf6N2E2_4447 at 5178.034 kb on + strand, within Pf6N2E2_4447 at 5178.035 kb on - strand, within Pf6N2E2_4447 at 5178.035 kb on - strand, within Pf6N2E2_4447 at 5178.035 kb on - strand, within Pf6N2E2_4447 at 5178.035 kb on - strand, within Pf6N2E2_4447 at 5178.091 kb on + strand, within Pf6N2E2_4447 at 5178.091 kb on + strand, within Pf6N2E2_4447 at 5178.091 kb on + strand, within Pf6N2E2_4447 at 5178.214 kb on + strand, within Pf6N2E2_4447 at 5178.214 kb on + strand, within Pf6N2E2_4447 at 5178.215 kb on - strand, within Pf6N2E2_4447 at 5178.223 kb on + strand, within Pf6N2E2_4447 at 5178.223 kb on + strand, within Pf6N2E2_4447 at 5178.223 kb on + strand, within Pf6N2E2_4447 at 5178.223 kb on + strand, within Pf6N2E2_4447 at 5178.223 kb on + strand, within Pf6N2E2_4447 at 5178.224 kb on - strand, within Pf6N2E2_4447 at 5178.224 kb on - strand, within Pf6N2E2_4447 at 5178.224 kb on - strand, within Pf6N2E2_4447 at 5178.224 kb on - strand, within Pf6N2E2_4447 at 5178.224 kb on - strand, within Pf6N2E2_4447 at 5178.224 kb on - strand, within Pf6N2E2_4447 at 5178.258 kb on + strand, within Pf6N2E2_4447 at 5178.258 kb on + strand, within Pf6N2E2_4447 at 5178.258 kb on + strand, within Pf6N2E2_4447 at 5178.259 kb on - strand, within Pf6N2E2_4447 at 5178.313 kb on + strand, within Pf6N2E2_4447 at 5178.313 kb on + strand, within Pf6N2E2_4447 at 5178.313 kb on + strand, within Pf6N2E2_4447 at 5178.313 kb on + strand, within Pf6N2E2_4447 at 5178.313 kb on + strand, within Pf6N2E2_4447 at 5178.314 kb on - strand, within Pf6N2E2_4447 at 5178.314 kb on - strand, within Pf6N2E2_4447 at 5178.314 kb on - strand, within Pf6N2E2_4447 at 5178.314 kb on - strand, within Pf6N2E2_4447 at 5178.314 kb on - strand, within Pf6N2E2_4447 at 5178.314 kb on - strand, within Pf6N2E2_4447 at 5178.499 kb on + strand, within Pf6N2E2_4447 at 5178.536 kb on - strand, within Pf6N2E2_4447 at 5178.562 kb on + strand, within Pf6N2E2_4447 at 5178.562 kb on + strand, within Pf6N2E2_4447 at 5178.562 kb on + strand, within Pf6N2E2_4447 at 5178.563 kb on - strand, within Pf6N2E2_4447 at 5178.563 kb on - strand, within Pf6N2E2_4447 at 5178.563 kb on - strand, within Pf6N2E2_4447 at 5178.563 kb on - strand, within Pf6N2E2_4447 at 5178.563 kb on - strand, within Pf6N2E2_4447 at 5178.563 kb on - strand, within Pf6N2E2_4447 at 5178.583 kb on + strand, within Pf6N2E2_4447 at 5178.583 kb on + strand, within Pf6N2E2_4447 at 5178.583 kb on + strand, within Pf6N2E2_4447 at 5178.584 kb on - strand, within Pf6N2E2_4447 at 5178.670 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.796 kb on + strand, within Pf6N2E2_4447 at 5178.797 kb on - strand, within Pf6N2E2_4447 at 5178.797 kb on - strand, within Pf6N2E2_4447 at 5178.797 kb on - strand, within Pf6N2E2_4447 at 5178.797 kb on - strand, within Pf6N2E2_4447 at 5178.797 kb on - strand, within Pf6N2E2_4447 at 5178.798 kb on + strand, within Pf6N2E2_4447 at 5178.798 kb on + strand, within Pf6N2E2_4447 at 5178.798 kb on + strand, within Pf6N2E2_4447 at 5178.798 kb on + strand, within Pf6N2E2_4447 at 5178.798 kb on + strand, within Pf6N2E2_4447 at 5178.798 kb on + strand, within Pf6N2E2_4447 at 5178.798 kb on + strand, within Pf6N2E2_4447 at 5178.799 kb on - strand, within Pf6N2E2_4447 at 5178.799 kb on - strand, within Pf6N2E2_4447 at 5178.799 kb on - strand, within Pf6N2E2_4447 at 5178.799 kb on - strand, within Pf6N2E2_4447 at 5178.799 kb on - strand, within Pf6N2E2_4447 at 5178.967 kb on + strand, within Pf6N2E2_4447 at 5178.967 kb on + strand, within Pf6N2E2_4447 at 5178.967 kb on + strand, within Pf6N2E2_4447 at 5178.967 kb on + strand, within Pf6N2E2_4447 at 5178.967 kb on + strand, within Pf6N2E2_4447 at 5178.967 kb on + strand, within Pf6N2E2_4447 at 5178.968 kb on - strand, within Pf6N2E2_4447 at 5179.096 kb on + strand, within Pf6N2E2_4447 at 5179.096 kb on + strand, within Pf6N2E2_4447 at 5179.096 kb on + strand, within Pf6N2E2_4447 at 5179.373 kb on + strand, within Pf6N2E2_4447 at 5179.373 kb on + strand, within Pf6N2E2_4447 at 5179.374 kb on - strand, within Pf6N2E2_4447 at 5179.374 kb on - strand, within Pf6N2E2_4447 at 5179.525 kb on + strand at 5179.525 kb on + strand at 5179.526 kb on - strand at 5179.573 kb on + strand at 5179.574 kb on - strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.620 kb on + strand at 5179.621 kb on - strand at 5179.621 kb on - strand at 5179.621 kb on - strand at 5179.662 kb on - strand at 5179.827 kb on + strand, within Pf6N2E2_4448 at 5179.856 kb on + strand, within Pf6N2E2_4448 at 5179.857 kb on - strand, within Pf6N2E2_4448 at 5180.061 kb on - strand, within Pf6N2E2_4448 at 5180.176 kb on - strand at 5180.264 kb on - strand, within Pf6N2E2_4449 at 5180.264 kb on - strand, within Pf6N2E2_4449 at 5180.280 kb on + strand, within Pf6N2E2_4449 at 5180.280 kb on + strand, within Pf6N2E2_4449 at 5180.280 kb on + strand, within Pf6N2E2_4449 at 5180.280 kb on + strand, within Pf6N2E2_4449 at 5180.280 kb on + strand, within Pf6N2E2_4449 at 5180.280 kb on + strand, within Pf6N2E2_4449 at 5180.280 kb on + strand, within Pf6N2E2_4449 at 5180.281 kb on - strand, within Pf6N2E2_4449 at 5180.281 kb on - strand, within Pf6N2E2_4449 at 5180.281 kb on - strand, within Pf6N2E2_4449 at 5180.281 kb on - strand, within Pf6N2E2_4449 at 5180.421 kb on + strand, within Pf6N2E2_4449 at 5180.422 kb on - strand, within Pf6N2E2_4449
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 5,176,805 + Pf6N2E2_4445 0.60 -0.8 5,176,806 - Pf6N2E2_4445 0.60 +0.8 5,176,806 - Pf6N2E2_4445 0.60 +0.6 5,177,013 + Pf6N2E2_4445 0.75 +0.6 5,177,014 - Pf6N2E2_4445 0.75 -1.5 5,177,048 + Pf6N2E2_4445 0.77 +1.8 5,177,049 - Pf6N2E2_4445 0.77 -0.1 5,177,049 - Pf6N2E2_4445 0.77 +0.8 5,177,049 - Pf6N2E2_4445 0.77 +0.3 5,177,171 + Pf6N2E2_4445 0.86 +2.5 5,177,171 + Pf6N2E2_4445 0.86 +2.7 5,177,172 - Pf6N2E2_4445 0.86 -1.5 5,177,172 - Pf6N2E2_4445 0.86 -0.7 5,177,174 + Pf6N2E2_4445 0.86 +0.9 5,177,174 + Pf6N2E2_4445 0.86 +1.7 5,177,174 + Pf6N2E2_4445 0.86 +2.5 5,177,174 + Pf6N2E2_4445 0.86 +0.7 5,177,174 + Pf6N2E2_4445 0.86 +2.0 5,177,174 + Pf6N2E2_4445 0.86 +0.9 5,177,175 - Pf6N2E2_4445 0.86 +0.0 5,177,175 - Pf6N2E2_4445 0.86 +0.1 5,177,175 - Pf6N2E2_4445 0.86 -1.1 5,177,175 - Pf6N2E2_4445 0.86 -0.5 5,177,175 - Pf6N2E2_4445 0.86 -0.5 5,177,282 + +2.6 5,177,282 + +0.8 5,177,430 + +2.5 5,177,431 - -0.8 5,177,464 + +2.8 5,177,465 - +0.2 5,177,498 + +2.3 5,177,498 + +0.9 5,177,498 + -0.3 5,177,499 - +1.2 5,177,511 + +1.5 5,177,511 + +1.4 5,177,511 + +1.2 5,177,511 + +1.4 5,177,511 + +0.7 5,177,512 - -0.5 5,177,515 + +1.4 5,177,516 - +1.0 5,177,709 - Pf6N2E2_4446 0.36 -0.5 5,177,783 + -1.0 5,177,783 + +0.1 5,177,783 + +0.2 5,177,791 + +0.5 5,177,791 + -0.6 5,177,792 - -1.3 5,178,034 + Pf6N2E2_4447 0.14 -1.3 5,178,034 + Pf6N2E2_4447 0.14 -0.8 5,178,034 + Pf6N2E2_4447 0.14 -0.8 5,178,034 + Pf6N2E2_4447 0.14 -1.3 5,178,034 + Pf6N2E2_4447 0.14 -0.8 5,178,034 + Pf6N2E2_4447 0.14 -1.0 5,178,034 + Pf6N2E2_4447 0.14 -0.4 5,178,035 - Pf6N2E2_4447 0.14 -2.2 5,178,035 - Pf6N2E2_4447 0.14 -0.1 5,178,035 - Pf6N2E2_4447 0.14 -1.3 5,178,035 - Pf6N2E2_4447 0.14 -1.0 5,178,091 + Pf6N2E2_4447 0.17 -1.4 5,178,091 + Pf6N2E2_4447 0.17 -1.2 5,178,091 + Pf6N2E2_4447 0.17 -0.7 5,178,214 + Pf6N2E2_4447 0.24 +0.4 5,178,214 + Pf6N2E2_4447 0.24 +0.1 5,178,215 - Pf6N2E2_4447 0.24 -2.1 5,178,223 + Pf6N2E2_4447 0.24 -0.3 5,178,223 + Pf6N2E2_4447 0.24 -1.8 5,178,223 + Pf6N2E2_4447 0.24 -2.2 5,178,223 + Pf6N2E2_4447 0.24 +0.3 5,178,223 + Pf6N2E2_4447 0.24 +1.0 5,178,224 - Pf6N2E2_4447 0.25 +0.3 5,178,224 - Pf6N2E2_4447 0.25 +0.3 5,178,224 - Pf6N2E2_4447 0.25 -2.2 5,178,224 - Pf6N2E2_4447 0.25 +0.3 5,178,224 - Pf6N2E2_4447 0.25 -0.9 5,178,224 - Pf6N2E2_4447 0.25 -0.4 5,178,258 + Pf6N2E2_4447 0.26 -0.9 5,178,258 + Pf6N2E2_4447 0.26 -1.7 5,178,258 + Pf6N2E2_4447 0.26 -2.7 5,178,259 - Pf6N2E2_4447 0.27 -1.1 5,178,313 + Pf6N2E2_4447 0.30 -0.8 5,178,313 + Pf6N2E2_4447 0.30 -0.8 5,178,313 + Pf6N2E2_4447 0.30 +0.5 5,178,313 + Pf6N2E2_4447 0.30 -1.3 5,178,313 + Pf6N2E2_4447 0.30 +0.6 5,178,314 - Pf6N2E2_4447 0.30 -1.5 5,178,314 - Pf6N2E2_4447 0.30 -1.5 5,178,314 - Pf6N2E2_4447 0.30 -1.1 5,178,314 - Pf6N2E2_4447 0.30 +0.9 5,178,314 - Pf6N2E2_4447 0.30 -1.7 5,178,314 - Pf6N2E2_4447 0.30 -1.5 5,178,499 + Pf6N2E2_4447 0.40 +0.3 5,178,536 - Pf6N2E2_4447 0.42 -0.0 5,178,562 + Pf6N2E2_4447 0.43 -0.7 5,178,562 + Pf6N2E2_4447 0.43 -1.0 5,178,562 + Pf6N2E2_4447 0.43 -1.7 5,178,563 - Pf6N2E2_4447 0.44 -1.4 5,178,563 - Pf6N2E2_4447 0.44 +0.0 5,178,563 - Pf6N2E2_4447 0.44 +0.8 5,178,563 - Pf6N2E2_4447 0.44 +0.3 5,178,563 - Pf6N2E2_4447 0.44 -1.5 5,178,563 - Pf6N2E2_4447 0.44 -1.4 5,178,583 + Pf6N2E2_4447 0.45 -0.2 5,178,583 + Pf6N2E2_4447 0.45 -0.2 5,178,583 + Pf6N2E2_4447 0.45 +0.0 5,178,584 - Pf6N2E2_4447 0.45 -1.3 5,178,670 + Pf6N2E2_4447 0.49 +0.0 5,178,796 + Pf6N2E2_4447 0.57 -0.6 5,178,796 + Pf6N2E2_4447 0.57 -0.1 5,178,796 + Pf6N2E2_4447 0.57 -0.2 5,178,796 + Pf6N2E2_4447 0.57 -1.9 5,178,796 + Pf6N2E2_4447 0.57 -1.1 5,178,796 + Pf6N2E2_4447 0.57 -1.0 5,178,796 + Pf6N2E2_4447 0.57 -0.7 5,178,796 + Pf6N2E2_4447 0.57 -3.1 5,178,796 + Pf6N2E2_4447 0.57 +0.1 5,178,796 + Pf6N2E2_4447 0.57 -0.7 5,178,797 - Pf6N2E2_4447 0.57 -0.8 5,178,797 - Pf6N2E2_4447 0.57 -1.3 5,178,797 - Pf6N2E2_4447 0.57 -1.3 5,178,797 - Pf6N2E2_4447 0.57 -0.5 5,178,797 - Pf6N2E2_4447 0.57 +0.6 5,178,798 + Pf6N2E2_4447 0.57 +0.1 5,178,798 + Pf6N2E2_4447 0.57 -1.7 5,178,798 + Pf6N2E2_4447 0.57 -2.3 5,178,798 + Pf6N2E2_4447 0.57 +1.5 5,178,798 + Pf6N2E2_4447 0.57 -0.4 5,178,798 + Pf6N2E2_4447 0.57 -0.0 5,178,798 + Pf6N2E2_4447 0.57 -0.8 5,178,799 - Pf6N2E2_4447 0.57 -1.1 5,178,799 - Pf6N2E2_4447 0.57 +0.0 5,178,799 - Pf6N2E2_4447 0.57 -0.8 5,178,799 - Pf6N2E2_4447 0.57 -0.2 5,178,799 - Pf6N2E2_4447 0.57 -0.1 5,178,967 + Pf6N2E2_4447 0.66 -0.5 5,178,967 + Pf6N2E2_4447 0.66 -0.0 5,178,967 + Pf6N2E2_4447 0.66 -1.8 5,178,967 + Pf6N2E2_4447 0.66 -0.0 5,178,967 + Pf6N2E2_4447 0.66 -1.2 5,178,967 + Pf6N2E2_4447 0.66 +0.2 5,178,968 - Pf6N2E2_4447 0.66 -1.0 5,179,096 + Pf6N2E2_4447 0.73 -1.2 5,179,096 + Pf6N2E2_4447 0.73 +0.5 5,179,096 + Pf6N2E2_4447 0.73 -1.5 5,179,373 + Pf6N2E2_4447 0.89 +0.5 5,179,373 + Pf6N2E2_4447 0.89 -1.7 5,179,374 - Pf6N2E2_4447 0.89 -2.0 5,179,374 - Pf6N2E2_4447 0.89 -1.1 5,179,525 + +0.0 5,179,525 + -1.9 5,179,526 - -2.0 5,179,573 + -1.2 5,179,574 - -0.8 5,179,620 + -0.6 5,179,620 + -0.1 5,179,620 + -0.8 5,179,620 + -0.6 5,179,620 + +0.8 5,179,620 + -0.2 5,179,620 + +1.5 5,179,620 + +0.8 5,179,620 + +0.4 5,179,621 - +0.4 5,179,621 - +0.3 5,179,621 - +0.7 5,179,662 - -0.7 5,179,827 + Pf6N2E2_4448 0.32 +0.2 5,179,856 + Pf6N2E2_4448 0.39 -0.3 5,179,857 - Pf6N2E2_4448 0.39 -1.0 5,180,061 - Pf6N2E2_4448 0.84 -1.3 5,180,176 - +0.1 5,180,264 - Pf6N2E2_4449 0.11 +0.2 5,180,264 - Pf6N2E2_4449 0.11 -0.4 5,180,280 + Pf6N2E2_4449 0.14 +0.6 5,180,280 + Pf6N2E2_4449 0.14 +0.6 5,180,280 + Pf6N2E2_4449 0.14 -0.3 5,180,280 + Pf6N2E2_4449 0.14 -1.1 5,180,280 + Pf6N2E2_4449 0.14 -0.2 5,180,280 + Pf6N2E2_4449 0.14 -1.0 5,180,280 + Pf6N2E2_4449 0.14 +0.5 5,180,281 - Pf6N2E2_4449 0.14 -1.3 5,180,281 - Pf6N2E2_4449 0.14 +0.0 5,180,281 - Pf6N2E2_4449 0.14 -0.7 5,180,281 - Pf6N2E2_4449 0.14 +0.6 5,180,421 + Pf6N2E2_4449 0.35 -0.8 5,180,422 - Pf6N2E2_4449 0.35 +1.3
Or see this region's nucleotide sequence