Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_33 and Pf6N2E2_34 are separated by 56 nucleotides Pf6N2E2_34 and Pf6N2E2_35 are separated by 12 nucleotides
Pf6N2E2_33 - Diaminopimelate epimerase (EC 5.1.1.7), at 71,998 to 72,999
_33
Pf6N2E2_34 - Cytosine deaminase (EC 3.5.4.1), at 73,056 to 74,303
_34
Pf6N2E2_35 - Cytosine permease, at 74,316 to 75,587
_35
Position (kb)
73
74
75 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 72.255 kb on + strand, within Pf6N2E2_33 at 72.694 kb on + strand, within Pf6N2E2_33 at 72.695 kb on - strand, within Pf6N2E2_33 at 73.130 kb on - strand at 73.162 kb on + strand at 73.163 kb on - strand at 73.340 kb on + strand, within Pf6N2E2_34 at 73.458 kb on - strand, within Pf6N2E2_34 at 73.549 kb on + strand, within Pf6N2E2_34 at 73.550 kb on - strand, within Pf6N2E2_34 at 73.561 kb on + strand, within Pf6N2E2_34 at 73.562 kb on - strand, within Pf6N2E2_34 at 73.756 kb on + strand, within Pf6N2E2_34 at 73.756 kb on + strand, within Pf6N2E2_34 at 74.317 kb on + strand at 74.317 kb on + strand at 74.359 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.378 kb on - strand at 74.378 kb on - strand at 74.378 kb on - strand at 74.378 kb on - strand at 74.593 kb on + strand, within Pf6N2E2_35 at 74.594 kb on - strand, within Pf6N2E2_35 at 74.612 kb on - strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.717 kb on - strand, within Pf6N2E2_35 at 74.717 kb on - strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.822 kb on - strand, within Pf6N2E2_35 at 75.107 kb on - strand, within Pf6N2E2_35 at 75.107 kb on - strand, within Pf6N2E2_35 at 75.139 kb on + strand, within Pf6N2E2_35 at 75.140 kb on - strand, within Pf6N2E2_35 at 75.140 kb on - strand, within Pf6N2E2_35 at 75.211 kb on + strand, within Pf6N2E2_35 at 75.211 kb on + strand, within Pf6N2E2_35 at 75.211 kb on + strand, within Pf6N2E2_35 at 75.212 kb on - strand, within Pf6N2E2_35 at 75.212 kb on - strand, within Pf6N2E2_35 at 75.253 kb on + strand, within Pf6N2E2_35
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 72,255 + Pf6N2E2_33 0.26 -0.2 72,694 + Pf6N2E2_33 0.69 -1.1 72,695 - Pf6N2E2_33 0.70 -0.5 73,130 - -0.1 73,162 + -0.9 73,163 - -1.5 73,340 + Pf6N2E2_34 0.23 -1.6 73,458 - Pf6N2E2_34 0.32 -0.0 73,549 + Pf6N2E2_34 0.40 -0.7 73,550 - Pf6N2E2_34 0.40 -0.3 73,561 + Pf6N2E2_34 0.40 -0.2 73,562 - Pf6N2E2_34 0.41 -0.6 73,756 + Pf6N2E2_34 0.56 -0.8 73,756 + Pf6N2E2_34 0.56 -0.7 74,317 + -0.0 74,317 + +0.3 74,359 - -0.8 74,371 - +0.8 74,371 - -0.5 74,371 - -0.4 74,371 - +0.8 74,371 - -0.3 74,377 + -0.6 74,377 + -2.2 74,377 + -1.0 74,377 + -0.4 74,377 + -0.8 74,377 + +0.8 74,378 - +0.8 74,378 - -1.1 74,378 - -1.7 74,378 - -0.4 74,593 + Pf6N2E2_35 0.22 -0.2 74,594 - Pf6N2E2_35 0.22 -0.6 74,612 - Pf6N2E2_35 0.23 +0.9 74,716 + Pf6N2E2_35 0.31 -1.5 74,716 + Pf6N2E2_35 0.31 +0.1 74,716 + Pf6N2E2_35 0.31 -1.2 74,716 + Pf6N2E2_35 0.31 +1.2 74,716 + Pf6N2E2_35 0.31 -0.8 74,717 - Pf6N2E2_35 0.32 +0.1 74,717 - Pf6N2E2_35 0.32 -0.2 74,821 + Pf6N2E2_35 0.40 +0.1 74,821 + Pf6N2E2_35 0.40 +0.2 74,821 + Pf6N2E2_35 0.40 +0.2 74,821 + Pf6N2E2_35 0.40 -0.2 74,821 + Pf6N2E2_35 0.40 +0.5 74,822 - Pf6N2E2_35 0.40 +1.4 75,107 - Pf6N2E2_35 0.62 -0.4 75,107 - Pf6N2E2_35 0.62 +0.6 75,139 + Pf6N2E2_35 0.65 +0.8 75,140 - Pf6N2E2_35 0.65 -0.4 75,140 - Pf6N2E2_35 0.65 -1.0 75,211 + Pf6N2E2_35 0.70 -0.2 75,211 + Pf6N2E2_35 0.70 +0.1 75,211 + Pf6N2E2_35 0.70 -0.8 75,212 - Pf6N2E2_35 0.70 +0.2 75,212 - Pf6N2E2_35 0.70 +1.2 75,253 + Pf6N2E2_35 0.74 +2.8
Or see this region's nucleotide sequence